Plant Preservative Mixture (PPM™)

Plant Preservative Mixture (PPM™)Plant Preservative Mixture (PPM), is a broad spectrum biocide for plant tissue culture. PPM is the ultimate solution to the never ending struggle against microbial airborne, waterborne, and endogenous contamination. Coupled with proper observation of aseptic laboratory techniques, PPM can increase your chances of a successful and thriving culture experiment. The Benefits of Plant Preservative Mixture At optimum doses, PPM, which stands for Plant

Plant Preservative Mixture (PPM™), is a broad-spectrum biocide for plant tissue culture. PPM™ is the ultimate solution to the never-ending struggle against microbial airborne, waterborne, and endogenous contamination.

Coupled with proper observation of aseptic laboratory techniques, PPM™ can increase your chances of a successful and thriving culture experiment.

The Benefits of Plant Preservative Mixture

• At optimum doses, PPM™, which stands for Plant Preservative Mixture™, is an extremely effective Preservative/Biocide, yet does not impair in vitro seed germination, callus proliferation and callus regeneration.
• PPM™ prevents the germination of both bacteria and fungi spores. It is heat stable and therefore can be autoclaved with the media.
• PPM™ can be, and should be used as a standard ingredient in plant tissue culture media, and is also substantially less expensive than commonly used antibiotics.
• While PPM™ was principally designed to inhibit airborne contamination, waterborne contamination, and contamination introduced from human contact, it can also -- in many cases -- be used to reduce endogenous contamination.
• PPM™ does not affect the genetic profile of the plants.

How To Use Plant Preservative Mixture?

1. General Dosage levels:

With the exception of endogenous contamination, the recommended dose range is 0.05%-0.2%. (For callus proliferation, organogenesis and embryogenesis, the recommended range is 0.05-0.075%.) To eliminate higher endogenous contamination densities, higher doses of PPM™ are needed.

2. Endogenous Contamination:

(a) For explants: gently and routinely shake / stir 1 cm. long explants (or shorter) for 4-12 hours in 4-5% v/v PPM™ solution supplemented as above with 3x MS
strength basal salts without Tween 20.Without rinsing, insert into a medium supplemented with 0.05 - 0.1% PPM™ for herbaceous plants and 0.2% PPM™ for woody plants.  

Note: Paragraphs 6(b) through 10 below are intended for ornamental plants only.

(b)For tubers, bulbs and scales: shake / stir the entire tuber/ bulb / scale in bleach. Rinse with water (can be done under non-sterile conditions). Slice the tuber / bulb /scale to thin slices. Shake / stir for 12-24 hours in 4- 5% PPM™ solution supplemented with full strength basal salts without pH ing & Tween 20. Without rinsing, insert into a medium supplemented with 0.1 -0.2% PPM™.