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Description
Histone H3 (acetyl K27) Recombinant Rabbit mAb (S-699-50)Product Specification Host Rabbit Antigen Histone H3 (acetyl K27) Synonyms H3K27ac Immunogen Synthetic Peptide Location Nucleus Accession P68431 Clone Number S 699 50 Antibody Type Recombinant mAb Isotype IgG Application WB, IHC P, ICC, ICFCM, IP, ChIP Reactivity Hu, Ms, Rt Predicted Reactivity Fu, Pl, Ys, SeUr, Dr, C. el, Hm, Or, Bv, Xe Purification Protein A Concentration 0. 5 mg ml Conjugation Unconjugated Physical Appearance Liquid Storage Buffer
Product Specification
| Host | Rabbit |
| Antigen | Histone H3 (acetyl K27) |
| Synonyms | H3K27ac |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P68431 |
| Clone Number | S-699-50 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, ICFCM, IP, ChIP |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Fu, Pl, Ys, SeUr, Dr, C.el, Hm, Or, Bv, Xe |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | |
| IP | 1:50 | |
| IHC-P | 1:500 | |
| ICC | 1:500 | |
| ICFCM | 1:50 | |
| ChIP | 1:20~1:50 |
Background
H3K27ac is an epigenetic modification to the DNA packaging protein histone H3. It is a mark that indicates acetylation of the lysine residue at N-terminal position 27 of the histone H3 protein. H3K27ac is associated with the higher activation of transcription and therefore defined as an active enhancer mark. H3K27ac is found at both proximal and distal regions of transcription start site (TSS). H3K27ac is also enriched in the regulatory regions of genes implicated in Alzheimer's disease, including those in tau and amyloid neuropathology.
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