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Description
Mouse REN ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. 3. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 1000pg/mL). Then dilute to the following concentrations: 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.625pg/mL, and 0pg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 1000pg/mL standard working solution into the first EP tube and mix thoroughly to make a 500pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with a Renin (REN) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Renin (REN) in the sample. The absorbance (OD value) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Renin ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Renin, also known as angiotensinogenase, is secreted by juxtaglomerular cells. These cells sense changes in renal perfusion pressure via stretch receptors in the vascular wall. Juxtaglomerular cells are also stimulated to release renin by signals from the macula. The macula senses changes in sodium delivery to the distal tubules and responds to the decrease in tubular sodium load by stimulating juxtaglomerular cells to release renin. The macula and paraglomerular cells together form the paraglomerular complex. Renin secretion is also stimulated by sympathetic nerves, primarily through activation of β1-adrenergic receptors. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 15.62-1000 pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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4.9 ★★★★★
Based on 22 reviews
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Product Reviews
★★★★★ 5
Luxuriously Soft and Packaged Like a High-End Brand
Color: 03 - Champagne, Size: Queen, Color: 03 - Champagne, Size: Queen
I have never owned a 100% silk pillowcase before, especially not at this price point, so I am really glad I was able to experience this one. Even the packaging felt impressive. It was packaged like a luxury brand, which made the whole experience feel special before I even used it.
I already had satin pillowcases and thought they were soft, but they do not compare to this mulberry silk pillowcase at all. This pillowcase feels incredibly soft, smooth, and luxurious. I had the satin pillowcases on some of my pillows and this silk one on another, and even in the dark when I turn over during the night, I can immediately tell which pillow has this silk pillowcase on it. The difference is that noticeable.
It also has ample room, so you can use a full, fluffy pillow and it will still fit nicely inside the pillowcase. I love the added luxury of the zipper because it keeps the pillow from slipping out, but the zipper is hidden and does not affect the comfort at all. Honestly, this pillowcase put my old flattened pillow to shame. Now I feel like I need to get a new pillow immediately so the pillow can match the quality of the pillowcase.
It feels so gentle and smooth against my face, and I love how cool it gets when the air conditioning hits it for a while. For nighttime hot flashes, that cool feeling is such a relief. I also really appreciate that I don't always have to sleep with a satin cap if I don't want to. I have locs, so protecting my hair from lint and dryness is important, but some nights you just want to be completely comfortable without wearing anything on your head. This pillowcase gives me that option while still being gentle on my hair.
It also feels better for my skin compared to cotton and other materials because it doesn't seem to soak up all the natural oils from my face. I notice a difference. The only thing I would mention is that while it gives a nice cool feeling at first, it does absorb body heat fairly quickly and can warm up. Even with that, the softness and comfort more than make up for it.
I didn't know what I was missing by not having real silk sheets, and now I really crave a full silk sheet and pillowcase set. If they feel anything like this pillowcase, I know that would be some of the best sleep ever. Overall, this pillowcase is soft, luxurious, comfortable, beautifully packaged, roomy, secure with the hidden zipper, and absolutely worth trying.
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Reviewed in the United States on May 27, 2026
★★★★★ 5
How do I know if this is ....
Color: 02 - Silver Cloud, Size: Queen, Color: 02 - Silver Cloud, Size: Queen
Mulberry Silk comes primarily from silkworms in China who eat white mulberry leaves, leaving a cocoon of spun silk that is used to weave fabric. With so many cheaper versions of mulberry silk, how do I know if this is the real deal? Well, I really do not know if this pillow fabric is 100% silkworm derived. It is not white so there had to be some level of dye added and that colorant is not listed. So, while I cannot confirm the fabric content, I can share how the pillow feels and functions.
I have had silk and satin pillows and went back to cotton because organic cotton simply felt better on my face while sleeping. This pillow case is not like other silks, satin, or cotton.
The fabric is smooth and soft - it's like sleeping on butter. I woke up this morning with less hair tangle and my face felt cuddled. I washed the pillow case before using it for the first time and the photo of this pillow case on my pillow (next to a red pillow) shows you how this pillow case looks right after being washed and fitted to a queen sized pillow.
The only downside to this pillow case is extra care is needed with washing and drying. I washed the pillow case in a mesh bag in cold water and let it hang dry. It's probably not ideal fabric if you have oily skin or go to sleep with makeup because you cannot use harsh detergents to wash this pillow case and you cannot use fabric softener. I tend to wash the rest of my bedding on high heat and dry at high heat to reduce dust mites and other tiny creatures we cannot see on fabric.
The zipper is good quality and easy to open and close - at this price point I would have expected a little better design to hide the metal zipper pull - added a photo to show how the metal zipper tab stops about 2 inches from the end of the pillow case with no "tuck-in" feature.
The value of this pillow case seems to be fair for a high end product and it makes a nice gift. I don't know anyone who doesn't appreciate buttery soft fabric. There are a ton of pillow cases that claim to be mulberry silk at a fraction of the cost of this pillow case - it's just so hard to know what is authentic and what is not so i can only speak to the fact that this pillow case feels good on my face and doesn't leave me with tangled hair.
Hope the photos and the description of my experience with this pillow help you decide if this is the right pillow for you or a loved one. Here's to your comfort!
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Reviewed in the United States on March 28, 2026
★★★★★ 5
A tangible upgrade for hair care and sleep quality
Color: 02 - Silver Cloud, Size: King
I bought this a few weeks back to integrate into a more refined sleep setup, and it has easily met my expectations. It's a premium product that delivers on its promises.
Material: You can immediately feel the difference in the 22 momme weight. It feels substantially thicker and more densely woven than typical "satin" substitutes, providing a smooth, glassy texture that feels truly high-end.
Hair Protection: This is where it really shines. The frictionless surface has completely eliminated morning tangles and frizz. It allows hair to glide naturally, which is a massive relief if you are trying to preserve hair health or styles overnight.
Comfort: It stays incredibly cool to the touch throughout the night. If you sleep hot, this naturally regulates temperature, meaning you never have to flip the pillow searching for the cold side.
Appearance: The Silver Cloud color is elegant, and the fabric has a subtle, sophisticated luster. While it does arrive with some packaging creases, they smooth out with a careful press, leaving a very polished look on the bed.
Fit: The King size accommodates larger, high-loft pillows perfectly without stretching. The hidden zipper is a great "zero-added-clicks" feature; it keeps the pillow locked in all night without any annoying slippage, though a slightly wider zipper opening would make insertion even easier.
Quality: The stitching is tight, and the zipper operates smoothly. It holds up beautifully when hand-washed and air-dried. While $70 is an investment for a single case, the genuine 6A grade silk and OEKO-TEX certification make it a worthwhile piece of luxury.
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Reviewed in the United States on May 1, 2026
★★★★★ 5
Cool, Quiet Luxury for Your Face (and Your Hair Thanks You Too)
Color: 01 - Ivory, Size: Queen, Color: 01 - Ivory, Size: Queen
This is one of those products where you immediately understand the price the second you touch it.
First off—this definitely IS real silk, not satin, which is important to verify (esp at this price point). There are so many listings on Amazon of bonnets and pillowcases, sheets etc claiming “silk” and when you get it, it’s satin. This pillowcase is 100% mulberry silk, and you can feel it immediately. What surprised me though is that I actually have other 100% mulberry silk items (my daughter’s play silks from Sarah’s Silks), and somehow this pillowcase feels way smoother and more luxurious than those. Same material on paper, totally different experience in real life.
From what I can tell, it’s thicker and more tightly woven, which gives it that extra silky, almost glassy feel. It’s buttery smooth, lightweight, and has that cool-to-the-touch finish that’s honestly a dream in the Phoenix, AZ heat, where it’s already 105 in March. Getting into bed feels like flipping your pillow to the cold side… every time. 🧊
It fits a standard queen pillow perfectly (mine’s a thicker foam pillow and slid right in with zero struggle), and the hidden zipper is secure without being annoying. No bunching, no slipping off mid-sleep—just stays put.
And it’s not just nice—it’s doing something. My hair definitely wakes up less chaotic, and it feels gentler on my skin overall. One of those quiet upgrades you don’t think about much… until you do.
The ivory color is soft and clean-looking, very effortless luxury, and I like that there are other color options if you want to switch up the vibe.
Yes, $60 for a pillowcase sounds a little wild—but this actually feels like a $60 pillowcase. Thick, high-quality, and genuinely luxurious, not pretending to be.
If you’ve been on the fence, this is one of those small changes that makes your whole sleep setup feel elevated.
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Reviewed in the United States on March 28, 2026
★★★★★ 1
Price
Color: 01 - Ivory, Size: Queen
Price way too high
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 27, 2026
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