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Description
Human CBR3 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Carbonyl Reductase 3 (CBR3). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Carbonyl Reductase 3 (CBR3) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Carbonyl Reductase 3 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Carbonyl reductase 3 (CBR3), also known as short-chain dehydrogenase/reductase family 21C member 2 (SDR21C2), is an enzyme encoded by the CBR3 gene. It catalyzes the reduction of a wide range of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. The enzyme is classified as a monomeric NADPH-dependent oxidoreductase. It comprises three exons spanning 11.2 kilobases and is closely linked to another carbonyl reductase gene, CBR1. It exhibits low NADPH-dependent oxidoreductase activity (in vitro) towards 4-benzoylpyridine and menadione. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.8 ★★★★★
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Product Reviews
★★★★★ 5
Love the storage and pull-out
Color: Dark Grey, Size: 7 Seat, Color: Dark Grey, Size: 7 Seat
The size is great and fits nicely in the corner without feeling too bulky, but still has plenty of seating. The dark grey color looks clean in person and matches my space really well — not too light, not too dark.
The pull-out part is honestly one of my favorite features. It slides out pretty smoothly and turns into a big lounging area or bed. I’ve already used it to relax and watch TV and it’s really comfortable. The storage compartment is also bigger than I expected — I can fit blankets and a few extra things in there easily, which helps keep everything more organized.
Comfort-wise, I’d say it’s medium-firm. Not super soft like a cloud couch, but still comfortable enough for everyday use. The cushions seem to hold their shape pretty well so far.
Assembly took a bit of time and it’s easier with two people, but overall it wasn’t too complicated. Once it’s set up, it feels pretty sturdy.
If I had to nitpick, the cushions do shift a little after sitting for a while, so you may need to adjust them occasionally. But for the price, I think that’s pretty normal.
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Reviewed in the United States on April 29, 2026
★★★★★ 4
very nice for the money
Color: Dark Grey, Size: 5 Seat, Color: Dark Grey, Size: 5 Seat
Very comfy. The color is perfect. The back pillows do shift when leaning against them but a throw pillow or two helps comfort. The material is soft. And it was fairly easy to assemble with 2 people.
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Reviewed in the United States on June 1, 2026
★★★★★ 5
Epic. Works great.
Color: Black
Works great every day. Super powerful and smooth compared to my old battery powered one. The shaft is much thicker and is more securely positioned so I can't imagine it becoming tweaked off balance like my old one. And it comes with an extra mixer attachment! I imagine I've put that away for a long time, but we'll see. Easy to turn on and off with one hand, but I can imagine it being less easy than a simple push button for someone with decreased thumb dexterity or mobility. I do wish the lowest speed was slightly lower, but it hasn't caused any splashing or other detriment. The speed adjustment is very smooth and power is abundant. Love the stand, Usb c charge port is solid, battery life is excellent.
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Reviewed in the United States on April 13, 2026
★★★★★ 5
You want this one, for power and quality and saving on AA battery costs
Color: Black
Yes. You want this one, for power and quality and saving on AA battery costs — buy it now. Don’t waste any more time comparing products.
After hours, days, weeks, of researching and comparing, I ordered this one. I do not regret it. It is powerful. It was less than $25 when I bought it. It has a USB charger port and it feels solid in your hand. It has a turn-knob at the top to control the variable speed immersion blender aka milk frother aka wand frother. Charge battery fully for 2hrs before first use and then you’re all set. It works very well. It functioned as expected and then even better. Yes. It exceeded expectations. I hope it lasts a long time. It feels like it is very well made and high quality. Classiest and best wand frother I have ever owned. And I’ve owned over 3-4 in the past 10yrs.
I got this one at $25 instead of the highest-rated one for $9.99 because those require AA batteries and this frother however is USB-C rechargeable!
So in 1-2 years it will have paid for itself in battery costs alone.
But there’s no price I can put on how enjoyable it is to use this super powerful, super smooth, high quality, wand frother.
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Reviewed in the United States on September 20, 2025
★★★★★ 5
Quality Design
Color: Gray
Excellent frother/mixer
Pros:
- Quality feels premium.
- Plenty of power to mix even with a drink packed with ice and peanut butter. Basically a mini handheld mixer.
- Ease of use is great. I appreciated that this had a longer brother so I can mix drinks in taller cups. Also the grip is fantastic and it is easy very easy to change the speed with just one hand so you can hold the drink with the other.
Longevity - So far it's been holding up and hasn't needed a battery replacement.
Cons:
None so far.
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Reviewed in the United States on December 10, 2025