SKU: 25552881680

Rat IgA OneStep ELISA Kit

Sale price$225.00 Regular price$250.00
Save 10%

Pay in installments of $62.50 with ShopPay, AfterPay and Klarna

Shipping Estimate
USA
  • USA
  • CAN

Ships within 48 hours · Estimated delivery Aug 26 - Aug 31

Promo Codes Available:

For Your Every Summer RSVP, with Code: SUMMER15

Description

Rat IgA OneStep ELISA KitProduct Specification Antigen IgA Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Rt Purification Protein A Stability & Storage 2 to 8 C as supplied. Kit Precision Intra assay: 5. 7% Inter assay: 7. 1% Sample type Cell culture supernatant, Serum, Plasm Assay type Sandwich (quantitative) Sensitivity 0. 043 ng ml Range 1. 56 ng mL 100ng mL Recovery Cell culture supernatant: 99% Serum: 90% Plasm: 115% Assay time 60 minutes Species

Product Specification


Antigen IgA
Immunogen Recombinant Protein
Antibody Type Recombinant mAb
Reactivity Rt
Purification Protein A
Stability & Storage 2 to 8 °C as supplied.

Kit


Precision Intra-assay: 5.7%;
Inter-assay: 7.1%
Sample type Cell culture supernatant, Serum, Plasm
Assay type Sandwich (quantitative)
Sensitivity 0.043 ng/ml
Range 1.56 ng/mL – 100ng/mL
Recovery Cell culture supernatant: 99%
Serum: 90%
Plasm: 115%
Assay time 60 minutes
Species reactivity Rat

Background

Immunoglobulin A (IgA) is a pivotal antibody isotype central to mucosal immunity and systemic immune regulation in rats. Structurally, rat IgA exists predominantly as a monomer in serum and as a dimer or polymer (secretory IgA, sIgA) at mucosal surfaces. Each IgA molecule comprises two α-heavy chains and two light chains (κ or λ), forming a flexible Y-shaped structure stabilized by disulfide bonds. The α-heavy chain contains three constant domains (Cα1–Cα3), with the Fc region (Cα2–Cα3) facilitating interactions with the Fcα receptor (FcαRI) and the polymeric immunoglobulin receptor (pIgR). Secretory IgA incorporates a J-chain and a secretory component, which enhance its stability in protease-rich mucosal environments. Extensive glycosylation of rat IgA further modulates its solubility, receptor binding, and resistance to enzymatic degradation. Rat IgA is the primary antibody safeguarding mucosal surfaces (e.g., respiratory, gastrointestinal tracts), where it neutralizes pathogens, blocks microbial adhesion, and maintains commensal microbiota balance. Secretory IgA mediates immune exclusion by trapping pathogens in mucus for mechanical clearance, while serum IgA contributes to systemic immune homeostasis by regulating inflammatory responses via FcαRI signaling. Additionally, rat IgA may engage in eosinophil-mediated antiparasitic defense and modulate allergic or autoimmune reactions through interactions with immune cells. Rat IgA shares conserved structural motifs with murine and human IgA, particularly in the α-chain constant domains. However, species-specific variations in glycosylation patterns, J-chain assembly, and secretory component binding necessitate species-specific assays for accurate detection. For example, rat IgA lacks the extended hinge region characteristic of human IgA1, resulting in distinct antigen accessibility and protease resistance. Clinical and Experimental Significance of IgA Levels: Elevated IgA: Associated with chronic mucosal infections (e.g., intestinal pathogens), autoimmune disorders (e.g., experimental colitis models), or inflammatory conditions. Reduced IgA: May indicate primary immunodeficiency (e.g., selective IgA deficiency in genetically modified models), immunosuppressive therapies, or gut dysbiosis. Applications of the Rat IgA ELISA Kit: This OneStep Rat IgA ELISA kit is designed for rapid and precise quantification of IgA in serum, plasma, or cell culture supernatants. Advantages of this OneStep ELISA Kit Design: Simplified Workflow: Combines sample incubation and detection in a single step, eliminating intermediate washing and reducing hands-on time. High Efficiency: Achieves results within 1 hours, ideal for high-throughput studies or urgent diagnostics. Enhanced Precision: Minimizes procedural variability and cross-contamination risks through optimized reagent formulation. Broad Compatibility: Validated for diverse biological matrices, ensuring flexibility in experimental design. This kit’s streamlined protocol and robust performance make it an indispensable tool for researchers and clinicians seeking reliable IgA quantification in time-sensitive or resource-limited settings.

Shipping Notes
  • Free Standard Shipping on $100+ Orders to the USA.
  • Except Preorder products are shipped in 48 hours.
  • Delivery to the USA:
  1. Standard Shipping : 3-10 business days
  • If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy
SKU: 25552881680

Discover Niche Categories That Outsell

Top-Converting Item to Boost Your Average Order

4.7 ★★★★★
Based on 16 reviews
Sort
Highest Rating
Newest First
Oldest First
Product Reviews
E
Verified Purchase
Eileen
Grantham, US
★★★★★ 5
Zoey loves this brand
Size: 1 Count (Pack of 1), Style: Heart
Our German Shepard, Zoey, loves the Spunky Pups squeaky toys. This heart shaped one is slightly smaller than the blue diamond one. Zoey likes to walk around everywhere with it in her mouth, so we prefer this one more since it’s not as big. The squeaking sound is medium-high pitched. It has great bounce as well. The toy overall is well made and will hold up over time.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on September 11, 2025
N
Verified Purchase
Noah Swift
Bozeman, US
★★★★★ 5
Dog loves them
Size: 1 Count (Pack of 1), Style: Heart
The dog loves these, they last about a month so not bad for the price, he loves to chew on them
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 10, 2026
L
Verified Purchase
Leanne Elizabeth
Pawtucket, US
★★★★★ 4
Squeaker Beware
Size: 1 Count (Pack of 1), Style: Heart
Bought these for gifts. One dog was squeaking it constantly (beware your ears lol) and within about 15mins it no longer squeaked. It was a Bernedoodle so wasn’t destroying it, just happily making it squeak. It still works as a bouncy ball. But the squeaker definitely doesn’t last long depending on how much it’s used. Overall a cute concept providing plenty of entertainment. Definitely not a toy if you have an aggressive chewer unless monitoring play.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 16, 2025
L
Verified Purchase
Ladidulce
Alexandria, US
★★★★★ 5
DOGS LOVE
Size: 1 Count (Pack of 1), Style: Heart
My dogs love it! I buy as gifts too as it’s always appreciated. Easy squeak and fun to bounce with all the sides. My dogs chew until they crack & still squeak for a bit. When eventually squeak stops my dogs still return to it. They seem to love just for the air sounds. I personally appreciates that more than the squeak but what do I know? I’m not a dog.6
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 9, 2025
C
Verified Purchase
C. Wisner
Bozeman, US
★★★★★ 1
Great, but they don't last.
Size: 1 Count (Pack of 1), Style: Heart
Our dog absolutely loves these things. However, they get punctured or form a crack on a seam with very little use. This time it only took a couple of hours of play. Once this happens, they no longer squeak. This may sound weird, but the heart shape seems worse for this defect.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 31, 2025

recommand products