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Description
SMAD4 Recombinant Rabbit mAb (SDT-168-67)Product Specification Host Rabbit Antigen Smad4 Synonyms Mothers against decapentaplegic homolog 4; MAD homolog 4; Mothers against DPP homolog 4; Deletion target in pancreatic carcinoma 4; hSMAD4 Immunogen Synthetic Peptide Location Cytoplasm, Nucleus Accession Q13485 Clone Number SDT 168 67 Antibody Type Recombinant mAb Isotype IgG Application WB, IP Reactivity Hu, Ms, Rt Predicted Reactivity Bv, Pg Purification Protein A Concentration 0. 5 mg ml
Product Specification
| Host | Rabbit |
| Antigen | Smad4 |
| Synonyms | Mothers against decapentaplegic homolog 4; MAD homolog 4; Mothers against DPP homolog 4; Deletion target in pancreatic carcinoma 4; hSMAD4 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q13485 |
| Clone Number | SDT-168-67 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IP |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Bv, Pg |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IP | 1:50 | Hu, Ms, Rt |
Background
SMAD (mothers against decapentaplegic homologs) molecules are the core components in TGF-β signaling pathway. TGF-β binding to its receptor induces phosphorylation and activation of receptor-regulated SMADs (R-SMADs), SMAD2 and SMAD3, which subsequently associate with their partner SMAD4 and translocate from cytoplasm to nucleus. Formation of R-SMAD–SMAD4 complexes is essential in signaling of most TGF-β family members.
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