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Description
Mouse TL ELISA KitProduct Specification Sensitivity 9. 375ng ml Species Reactivity Mouse Theory This kit uses competitive ELISA assay, and the experimental duration is 2 hours. The plates supplied in the kit have been pre coated with TC. The TC to be tested in the sample or standard will compete with the TC previously coated on the microplate for binding to a fixed amount of biotin labeled anti TC antibody. Free components are washed away. HRP streptavidin (SABC) is
Product Specification
| Sensitivity | 9.375ng/ml | ||||||||||||||||||||||||||||||||||
| Species Reactivity | Mouse | ||||||||||||||||||||||||||||||||||
| Theory | This kit uses competitive ELISA assay, and the experimental duration is 2 hours. The plates supplied in the kit have been pre-coated with TC. The TC to be tested in the sample or standard will compete with the TC previously coated on the microplate for binding to a fixed amount of biotin-labeled anti-TC antibody. Free components are washed away. HRP-streptavidin (SABC) is added, and biotin specifically binds to streptavidin to form an immune complex. The unbound conjugate was washed off and TMB chromogenic substrate was added. The TMB became blue under the catalysis of horseradish peroxidase (HRP) and turned yellow after the reaction stop solution was added. The OD value was measured at 450nm with a microplate reader. Using curve equation software, the OD450 value of the sample was compared to the standard curve to determine the concentration of TC in the sample. There is an inverse proportion between the concentration of the target substance and the OD450 value. | ||||||||||||||||||||||||||||||||||
| Synonym | Mouse TC (Total cholesterol) ELISA Kit | ||||||||||||||||||||||||||||||||||
| Detection Type | Competition Law | ||||||||||||||||||||||||||||||||||
| Composition |
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| General Notes | 1. When using different kits, it is necessary to mark them first to prevent the components from being mixed, resulting in experimental failure. 2. After the kit is opened, please refer to the component storage conditions table for the storage conditions of the enzyme plate and standard product (the activity will decrease after moisture). 3. Please use a sterile disposable tip to suck the reagent. After use, the reagent bottle cap must be tightened to prevent microbial contamination and evaporation. 4. When washing the plate manually, add the tip or dropper of the washing liquid and do not touch the hole of the enzyme labeled plate. Inadequate washing or contamination tend to cause false positives and high backgrounds. 5. During the testing process, please prepare the reagents required for the next experiment in advance, and add the reagents to the plate holes in time after washing the plate to prevent the plate holes from drying out and causing testing failure. 6. Do not use reagents from other batches of kits or reagents from other sources for this kit without confirmation. 7. Do not reuse disposable tips to avoid cross-contamination. 8. After the sample addition is completed, the film is applied to prevent the sample from evaporating during the incubation process, and the incubation process is completed at the recommended temperature. 9. Please wear laboratory coats, masks, gloves, etc. during the test, and do a good job of protection. Especially when testing blood or other body fluid samples, please follow the national biological laboratory safety protection regulations. |
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| Instructions | Serum, plasma, cell culture supernatant, cell lysate and other fluid samples | ||||||||||||||||||||||||||||||||||
| Storage Temp. | Unopened kit, stored at 2-8 °C, shelf life for one year. | ||||||||||||||||||||||||||||||||||
| Test Range | 15.625-1000ng/ml |
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★★★★★ 5
Exactly as described
Color: Red2
Very sturdy and definitely protects my iPad. Great buy even has a part of the pen. Fits perfectly no issues putting it on and it has layers to ensure that your iPad is protected. Color is exactly as pictured. Easy to use and charges fine with the case on
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Reviewed in the United States on March 18, 2026
★★★★★ 5
Not broken yet.
Color: Light Purple
I bought this for my daughter’s tablet and she has put it through the test. It has been dropped multiple times and the tablets still good. It was easy to put on, looks good, and does the job.
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Reviewed in the United States on April 26, 2026
★★★★★ 5
Durable and good case!
Color: Black
Excellent sturdy case to protect iPad. Would buy again!
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Reviewed in the United States on June 2, 2026
★★★★★ 5
Well made
Color: Blue
It’s great, better than I expected. I love I love the color and the fit is perfect. Highly recommend!
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Reviewed in the United States on May 30, 2026
★★★★★ 4
Good buy
Color: Black
Sturdy and fits perfectly
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Reviewed in the United States on May 6, 2026