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Description
Human S100A11 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with S100 Calcium Binding Protein A11 (S100A11) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of S100 Calcium Binding Protein A11 (S100A11) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human S100 Calcium Binding Protein A11 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | S100 calcium-binding protein A11 (S100A11) is a protein encoded by the S100A11 gene in humans. The protein encoded by this gene is a member of the S100 family and contains two EF-hand calcium-binding motifs. S100A11, also known as calgizzarin or 100C, is a small, acidic protein. It is located as a group on chromosome 1q21, along with all 13 members of the S100 family. This protein may play a role in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene are associated with tumor metastasis. High expression is found in many tissues, including the lung, pancreas, heart, placenta, and kidney, with lower expression in skeletal muscle, liver, and brain. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.7 ★★★★★
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Product Reviews
★★★★★ 5
Amazon Melatonin? Amazing!
These work as well or better than some of the more expensive ones my wife usually gets. They don't dissolve in your mouth and they arent gummies. No extra sugars involved. Puts me to sleep quite well and allows me to stay asleep. No bad taste, no weird dreams. No side effects.
Absolute gem of a find on Amazon. Great price for a great night sleep.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 7, 2026
★★★★★ 5
"Take this sinus infection!"
Size: 100 Count (Pack of 1)
I am a chronic sufferer of bacterial sinus infection. Ask me why, and I can give you some long winded explanation about a slightly deviated septum and sinus design, and a slew of other stuff that I've read and heard from my doctor. Bottom line, both you and I don't care the reasoning, but rather, preventative care. I can tell you that these Elderberry capsules help. If I get "that feeling" like a sinus infection is impending, I'll start popping two of these twice a day. 9 times out of 10, I can stave off the infection from grabbing a foothold in my sinuses long enough to let my immune system kick it completely out (without antibiotics and/or steroids).
Does it help get rid of a sinus infection already in place?: inconclusive from my experience. Once I have a sinus infection, it takes about the same amount of time to get rid of it whether or not I'm taking it. Maybe I get rid of it a day soon, but statistically that's probably considered to be "noise in the data".
Does it work for flu and other viral colds? I can't tell you that. I know that bacterial sinus infections can be precipitated by viral infections, but excluding bacterial sinus infection, I don't get sick. I've had the flu maybe twice in the last 20 years. Bottom line I have no experience with regard to using this to fight flu. Can't hurt to try though (I don't think you can OD on berries easily, could be wrong though).
Does it have any other benefits? I don't know, maybe. Again I can only speak from experience. I take them, it staves off a sinus infection. I get that there are a lot of other beneficial claims about elderberry, but I have personally never noticed/experienced them.
Is this just another homeopathic snake oil? No. While I agree that there are a lot of homeopathic "remedies" out there that don't work and are probably just a placebo effect, there are also ones out there that work. Heck, I've experienced allergic reactions to homeopathic remedies that landed me in worse trouble than before. Elderberry is a homeopathic remedy that works for me. Will it work for you? Maybe, maybe not, every body is different, and it could be that my body utilizes it due to a deficiency of something that elderberry has.
Summary (tl;dr): Get it and try the bottle once. You can take it all the time, but I find it sufficient to take them when I feel a sinus infection coming on (I mean, who *wants* to take a bunch of pills at breakfast). Berry form is probably preferable, but the capsules are convenient and store for long periods of time without getting mildew-y. The price is right, it staves off sinus infections, and probably works for staving off other colds as well. Can't hurt to find out if your body responds to it.
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Reviewed in the United States on October 3, 2013
★★★★★ 5
Highly recommended!
Size: 100 Count (Pack of 1)
Sometimes these can be a bit challenging to find in my local pharmacy. I like the price and the quality. As a senior citizen, I like to give my immune system a boost from time to time. I'm not convinced of the benefits, but I figure I can't go wrong by trying these.
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Reviewed in the United States on November 17, 2025
★★★★★ 5
A Trustworthy Immune Booster
Size: 100 Count (Pack of 1)
I've been really happy with this supplement. It's easy to take, and it's a trusted brand - great for peace of mind. The immune support it provides is exactly what I've been seeking. Overall, it's a quality product that I would recommend to others on the hunt for an effective supplement.
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Reviewed in the United States on September 29, 2024
★★★★★ 4
Look for other options if you have trouble swallowing pills.
Size: 100 Count (Pack of 1)
I bought these to replace a 30ct gummy bottle I bought for $9.99, thinking these capsules would be easier to swallow. I was wrong, very much so, for myself anyway.
I always have struggled swallowing pills to begin with, ever since I was a child. In order to swallow pills, I must either place them on my tongue and swallow with a liquid or I must take a drink first and drop the pill into my mouth. I do the latter for these, and while I've seen so many great reviews for this product, I think I'm going to need to find something else.
Because these come in capsules, they are buoyant. As soon as I put them in my mouth, they float to the roof, which is no big deal. However, they have a semi-foul taste to me and even using flavored drinks, the flavor overpowers my drink and has, on several occasions, gave me the feeling of being close to vomiting. I was super excited for these to help with fighting against all the virus and such circulating, and boosting my immune system. If you can handle larger pills, these will definitely do great for you. I, on the other hand, will need to find something smaller with less of a foul taste for me.
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Reviewed in the United States on January 31, 2020