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Description
Rat GSP ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as an anticoagulant and centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be collected for testing or stored at -20°C or -80°C, but avoid repeated freeze-thaw cycles. Pre-Assay Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let stand for 15 minutes to completely dissolve, then gently mix (concentration: 200 μg/mL). Then dilute to the following concentrations: 200 μg/mL, 100 μg/mL, 50 μg/mL, 25 μg/mL, 12.5 μg/mL, 6.25 μg/mL, 3.125 μg/mL, and 0 μg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 200ug/mL standard working solution into the first EP tube and mix thoroughly to make a 100ug/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against glycosylated serum protein (GSP). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of glycosylated serum protein (GSP) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Glycated serum protein ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Glycated serum protein (GSP) is similar to glycosylated hemoglobin, reflecting the average blood glucose concentration over the past 1-3 weeks. Since all glycosylated serum proteins are fructosamine, fructosamine measurement is considered to primarily measure glycosylated serum protein. Because serum protein synthesis is faster than hemoglobin (albumin has a half-life of approximately 20 days), glycosylated serum protein concentration reflects blood glucose levels over the past 1-3 weeks and reflects blood glucose control effectiveness earlier than glycosylated hemoglobin (GSP reflects the average blood glucose concentration over the past 8-12 weeks). In clinical practice, glycosylated serum protein and glycosylated hemoglobin are generally used in combination. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 3.12-200ug/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma |
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4.5 ★★★★★
Based on 27 reviews
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Product Reviews
★★★★★ 5
Incredible, perfect
Flavor Name: Zero Ultra, Size: 16 Fl oz (Pack of 15)
This is definitely the best flavor of Monster! No calories, but full flavor. I can't get enough of this stuff. It does cost a bit, but it is worth getting every once in awhile as a treat. I love it, and I would definitely recommend it. There is a high level of carbonation, so it doesn't go flat too quickly.
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Reviewed in the United States on May 24, 2026
★★★★★ 5
This Stuff Turned My Morning into a Rocket Launch – No Crash, Just Pure Chaos!
Flavor Name: Zero Ultra, Size: 16 Ounce (Pack of 24), Flavor Name: Zero Ultra, Size: 16 Ounce (Pack of 24)
I popped open my first can of this sparkling energy elixir expecting the usual lukewarm disappointment, but instead, it hit like a caffeinated unicorn tap-dancing on my taste buds. The flavor is ridiculously good – think tropical punch meets crisp citrus without that artificial "why did I do this" aftertaste. It's completely sugar-free, so no sneaky crash later, yet the carbonation is perfectly balanced: fizzy enough to feel exciting, but not so aggressive it turns your esophagus into a launchpad. Digestibility? Flawless. My stomach thanked me instead of staging a protest, which is a miracle for someone who once considered coffee a food group.
As for effectiveness, this bad boy delivers exactly what it promises and then some. The caffeine content is spot-on potent – enough to power through a soul-crushing workday or an impromptu home renovation project without the jitters or heart palpitations that usually come with high-octane drinks. I tackled my to-do list like a caffeinated superhero, all while staying hydrated and focused. Five stars for turning a regular Tuesday into an epic quest. If you're looking for clean, bubbly motivation in a can, stop scrolling and add this to your cart immediately. Your future self will high-five you.
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Reviewed in the United States on May 13, 2026
★★★★★ 5
Great taste without the sugar crash
Flavor Name: Zero Ultra, Size: 16 Fl oz (Pack of 15)
I picked this up because I wanted an energy drink that wouldn’t be loaded with sugar, and this one honestly surprised me. The flavor is really light and refreshing—not too sweet or overpowering like some other energy drinks.
I’ve been using it mainly in the mornings or when I need a quick boost, and it definitely helps with energy and focus without making me feel jittery. One thing I really like is that there’s no heavy aftertaste, which I’ve noticed with other sugar-free drinks.
Pros:
Zero sugar but still tastes good
Smooth, not too strong or artificial
Gives a noticeable energy boost
Cons:
Might be a little too mild if you like very strong flavors
The price can add up if you drink them often
Overall:
I’d recommend this if you want a cleaner-tasting energy drink without all the sugar. It’s easy to drink and does what it’s supposed to.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 3, 2026
★★★★★ 5
Great!
Flavor Name: Zero Ultra, Size: 16 Fl oz (Pack of 15)
Great price for this pack, saves money in the long run! The flavor is great and love the carbonation. The sugar free flavor is my favorite.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 28, 2026
★★★★★ 5
My go-to energy drink for years, no crashes or headaches
Flavor Name: Zero Ultra, Size: 16 Fl oz (Pack of 15)
Consistent energy, good taste, and no weird side effects
✔️ Sugar-free with no crash
✔️ No headaches or jittery feeling
✔️ Clean, smooth energy boost
✔️ Good flavor without being overly sweet
✔️ Reliable enough for long-term daily use
✔️ Subscription makes it easy to keep stocked
I’ve been buying Monster Zero Ultra for years and have it on subscription. It’s the one energy drink I consistently go back to. I've tried most others and they all leave me feeling weird.
I actually feel a noticeable energy boost from it, but without the downsides I’ve had with other drinks. No headaches, no weird jittery feeling, and no crash later on.
The flavor is also solid (this one and Ultra Violet are my go tos') , not overly sweet, and easy to drink regularly. I only buy sugar-free energy drinks, and this checks that box without sacrificing taste. I limit myself to one a day after the gym and water the rest of the day.
After trying a lot of different brands, this is the one that’s stayed consistent for me, which is why I’ve kept it on subscription.
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Reviewed in the United States on April 5, 2026
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