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Description
Human vitamin D ELISA KitProduct Specification protein VD Usage Specimen requirements: 1 . The specimen should be extracted as soon as possible after collection, and the extraction should be carried out according to the relevant literature. Experiments should be carried out as soon as possible after extraction. If the test cannot be carried out immediately, the specimen can be placed in 20 Store, but avoid repeated freezing and thawing 2 . Unable to detect containing NaN3
Product Specification
| protein | VD | ||||||||||||||||||||||||
| Usage | Specimen requirements: 1 . The specimen should be extracted as soon as possible after collection, and the extraction should be carried out according to the relevant literature. Experiments should be carried out as soon as possible after extraction. If the test cannot be carried out immediately, the specimen can be placed in -20℃ Store, but avoid repeated freezing and thawing 2 . Unable to detect containing NaN3 NaN3 Inhibition of horseradish peroxidase (HRP) activity. Operation steps: 1. Dilution of standard: This kit provides one original standard, which can be diluted in a small test tube according to the following chart.
2. Sample addition: Blank wells (no sample and enzyme labeled reagent are added to the blank control wells, and the other steps are the same), standard wells and sample wells to be tested are set respectively. Accurate spike of standard on enzyme-labeled coated plate 50uL, first add sample diluent to the sample well to be tested 40uL, then add the sample to be tested 10uL (The final dilution of sample was 5 Times). Add the sample to the bottom of the well of the enzyme labeled plate, try not to touch the well wall, and gently shake and mix well. 3. Incubation: After plate sealing with plate sealing film 37℃ Incubation 30 Minutes. 4. Solution preparation: will 30 Distilled water for double concentrated washing liquid 30 Time dilution for use 5. Washing: Carefully remove the sealing film, discard the liquid, spin dry, fill each well with washing liquid, and let stand 30 Discard it in seconds and repeat it 5 Times, pat dry. 6. Enzyme addition: enzyme labeled reagent is added to each well 50uL, except for blank holes. 7. Incubation: Same procedure 3. 8. Washing: Same operation 5. 9. Color development: Add color developer to each well first A 50uL And then adding a color developer B 50uL, gently shake and mix evenly, 37℃ Color development protected from light 10 Minutes. 10. Stop: Add stop solution per well 50uL, terminate the reaction (blue immediately turns yellow at this time). 11. Measurement: zeroing with blank holes, 450nm The absorbance of each well was measured sequentially by wavelength (OD Value). The determination shall be performed after addition of the stop solution 15 Within minutes. Calculation: Taking the concentration of the standard as the abscissa, OD The value is the ordinate, draw a standard curve on the coordinate paper, and according to the sample OD The corresponding concentration is found out from the standard curve; Multiply by the dilution factor; Or use the concentration of the standard substance to OD The linear regression equation of the standard curve is calculated from the value, and the sample OD The value is substituted into the equation, the sample concentration is calculated, and then multiplied by the dilution factor, which is the actual concentration of the sample. |
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| Species Reactivity | Human | ||||||||||||||||||||||||
| Theory | This kit uses double antibody sandwich method to determine the level of human vitamin D in specimens. Coating a microwell plate with purified human vitamin D antibody to make a solid phase antibody, adding vitamin D to the microwells coated with monoclonal antibody sequentially, and then combining with HRP-labeled vitamin D antibody to form an antibody-antigen-enzyme-labeled antibody complex, which is thoroughly washed and then added with substrate TMB to develop color. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. There was a positive correlation between the depth of color and vitamin D in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450nm, and the concentration of human vitamin D in the sample was calculated by the standard curve. | ||||||||||||||||||||||||
| Source | Human | ||||||||||||||||||||||||
| Synonym | Human VD ELISA Kit | ||||||||||||||||||||||||
| Composition | Kit composition:
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| General Notes | 1. The kit should be taken out of the refrigerated environment and balanced at room temperature for 1 hour before it can be used. If the enzyme-labeled coated plate is not used up after opening, the strips should be stored in a sealed bag. 2. Crystals may precipitate in the concentrated washing liquid. When diluting, it can be heated in a water bath to help dissolve, and the results will not be affected during washing. 3. A sampler should be used in each step of sampling, and its accuracy should be checked frequently to avoid test errors. It is best to control the sample addition time within 5 minutes. If the number of specimens is large, it is recommended to use a row gun to add samples. 4. Please make a standard curve at the same time of each measurement, preferably a double hole. If the content of the substance to be tested in the sample is too high (the OD value of the sample is greater than the OD value of the first well of the standard well), please dilute it with the sample diluent for a certain multiple (n times) before measuring, and please finally multiply it by the total dilution multiple (× n × 5) when calculating. 5. The sealing film is only for one-time use to avoid cross-contamination. 6. Please keep the substrate away from light. 7. Strictly follow the instructions, and the test results must be determined based on the reading of the microplate reader. 8. All samples, washing solutions and various wastes should be treated as infectious agents. 9. Components of different batch numbers of this reagent shall not be mixed. |
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| Storage Temp. | Store at 2-8 °C, shelf life 6 months. | ||||||||||||||||||||||||
| Test Range | 50ug/L -1600ug/L | ||||||||||||||||||||||||
| Applications | It is used to determine the content of vitamin D in human serum, plasma and related fluid samples. |
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4.7 ★★★★★
Based on 28 reviews
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Product Reviews
★★★★★ 4
Foes at the Top Table
Format: Kindle
Those of us who studied economics in the 60s grew up on Keynes. This book provides a fascinating picture of the great man in action. And an equally fascinating picture of the Lend Lease negotiations and then the US hard line at Bretton Woods. Behind this hard line was Harry Woods, of Lithuanian emigre stock, who clawed his way by hard work and intelligence to negotiating prominence in the US Treasury. And who was a Soviet agent of influence. Well written, lucid, and remarkably interesting.
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Reviewed in the United States on May 19, 2013
★★★★★ 5
A distant mirror of our current problems
Format: Hardcover
The title of this excellent book accurately describes its contents:it is about a battle fought to define a new world order, that which was emerging from the ashes of World War II. The book also conveys the messy complexity of such a historical process--how individual characters interpreted the events around them, realized that they were giving shape to a radically new future, tried to take advantage of them to advance their own personal and national interests, and succeeded in accordance with their intelligence, the cunning of their argumentation, and, above all, the shifts in the real power that supported them. Masterly, Benn Steil makes the reader feel how Keynes and White gradually reached an unspoken and unrecognized agreement regarding the shape that the new world would have, and then fought to gain advantage in that new world--Keynes trying to keep the British Empire paramount in the world order, now based not on the Royal Navy but on Britain's alliance with the United States, the emerging superpower, and White asserting the unimpeded power of the United States. Focusing on one crucial aspect of the new order, money, Steil is able to reenact the human drama of the transfer of world power from Britain to the United States in all orders of life. It is an excellent history book.
The book, however, goes beyond history as the narration and understanding of past events. When reading it, there is an eerie feeling that you are reading about current events. The process that led to Bretton Woods started thirty years before, with World War I and the end of the classical gold standard. When the war ended, a new monetary system was created, which was called the gold exchange standard. It resembled but emasculated the power of the old gold standard to keep monetary order in the world at large. This new system gave central banks the power to create money independently of the international consequences of doing it. With time, central banks abused this power, created a boom in the 1920s and then a depression in the 1930s. Bretton Woods was convened to reintroduce order in the monetary world. Like the gold standard of old, the new system created there was tied to gold in an effort to ensure stability. Yet, it also allowed central banks freedom to create money under certain circumstances. As it happened in the 1920s and 1930s, central banks abused their power, blew up the international system (in this case the Bretton Woods system) and then led the world into a series of booms and busts that has not ended as yet. A new monetary order will be needed to avoid worldwide inflation and protracted recessions. To understand the issues that will be crucial to give shape to this new monetary order it will be necessary to revisit the making of Bretton Woods in detail. There is no better way to understand these issues that Ben Steil's The Battle of Bretton Woods. Thus, in addition to being an excellent history book, it is also an excellent book about current events.
Full disclosure: I wrote a previous book with Benn Steil: Money, Markets and Sovereignty (Yale University Press, 2009).
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Reviewed in the United States on May 28, 2013
★★★★★ 5
The Battle of Bretton Woods
Format: Hardcover
From the growing reliance upon international finance and the devastating repercussions of two World Wars, Steil weaves together an important narrative that tells the story of America's rise to the world stage as a major power. Britain's reign of dominance comes to an abrupt end under the weight of the Second World War and the dependence of their territories. Reliance on foreign aid and mounting debt put Britain in a precarious situation for which the United States capitalized on to secure its place as the dominant world power. Through the ideas and experiences of two brilliant economists, Harry White and John Keynes, were guided through the creation and implementation of an economic solution to remedy, and further amalgamate the global financial framework. At Bretton Woods, White and Keynes promote slightly different plans that form the International Monetary Fund and World Bank; organizations designed to monitor, stabilize, and assist international finance. To Britain, and much of the world's chagrin, the organizations are formed in a manner that benefited the United States post-World War Two position as a creditor nation. Dollar dominance in the newly designed financial markets promotes short-term growth for the United States. However, financial mismanagement and over-extension soon lead the U.S. down a path of monetary hardship that ultimately results in our current situation as debtor nations (much like Britain was when the story began). The author ends the narrative by chronicling the effects of Bretton Woods on the United States, Britain, and international finance from the mid-twentieth century to the present. This book tells the remarkable story of America's rise to power through a financial lens. Steil is a wonderful writer who describes complex ideas of monetary policy, international economics, and currency manipulation in such a manner that is easy to understand and leaves the reader wanting more.
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Reviewed in the United States on August 16, 2013
★★★★★ 5
A concise, readable history of European developments prior to W.W. i.
Format: Paperback
This is part of a 4 volume series on (primarily) European history covering the development of the French and Industrial revolution(s) with particular emphasis on the Belle Epoque that marked the end of nearly 100 years of peace among the European Powers. It covers a variety of topics ranging from the emergence of the working classes; the role of the middle class; industrial capitalism; nationalism; the sciences and the arts; and, even a chapter on the "New Woman". Quite encompassing in its treatment and its analysis of the a period that serves as a background to the twentieth century.
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Reviewed in the United States on August 3, 2018
★★★★★ 5
Un comentario
Format: Paperback
El mundo burgués generó desarrollos muy importantes para los habitantes de Europa Occidental particularmente; incrementó de manera importante los niveles de vida de una porción significativa de la sociedad, introdujo innovaciones claras en las ciencias, en lo educativo, en el comercio global y en el intercambio entre diferentes culturas. Mejoró la productividad, las comunicaciones, la alimentación......Pero también incorporó consigo tendencias negativas y difíciles que cambiaron para siempre la interrelación entre pares al crearse una diferencia significativa de clases, la alta, burguesa y la trabajadora y proletaria, continuó la discriminación sobre la campesina, estableció el concepto forzado de nacionalismo, transformó a los países mas desarrollados en imperios con ansias de crecimiento y de dominio de los menos preparados, se evidenció la explotación del tercer mundo, se inició el armamentismo y la instigación a la guerra que culminó con la desastrosa confrontación de 1914. El mundo, a partir de ese momento no volverá a ser igual.
Todos estos conceptos, esta historia, están amenamente descritos por el autor, con un gran conocimiento de la época y con una inteligente asociación de los acontecimientos para hacer entender al lector sus grandes transformaciones e implicaciones.
Como los otros tres libros de la serie, lo recomiendo ampliamente. Permite entender todo la evolución de nuestra civilización en los dos últimos siglos así como la influencia generada por los grandes imperios de la época y sus pensadores
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Reviewed in the United States on November 28, 2011