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Description
Human IL4I1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and pipette tip: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serumplasm, aliquots, homogenates and other biological fluids Preparation before testing: 1. Please take out the test kit from the refrigerator 10 minutes in
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and pipette tip: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serumplasm, aliquots, homogenates and other biological fluids Preparation before testing: 1. Please take out the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 10 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Interleukin 4 Induced Protein 1 (IL4I1) capture antibody. After incubation and washing, the assay is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Interleukin 4 Induced Protein 1 (IL4I1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Interleukin 4 Induced Protein 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | IL4I1 (interleukin-4-induced protein 1) is a protein-coding gene. Diseases associated with IL4I1 include striatal degeneration, infantile and primary mediastinal large B-cell lymphoma. Pathways involved include the catabolism and metabolism of histidine, lysine, phenylalanine, tyrosine, proline, and tryptophan. Gene Ontology annotations associated with this gene include oxidoreductase activity and L-amino acid oxidase activity. An important homologue of this gene is KDM1A. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.0 ★★★★★
Based on 8 reviews
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Product Reviews
★★★★★ 5
Great for adult faces too
Size: 0.47 Ounce (Pack of 1)
Advertised for babies but this is my go to sunscreen for my face, especially when traveling. It is a stick so doesn't have to go in your liquids bag for TSA. No worrying about it leaking either. Easy to use, and works well. It does leave a whitish cast but that helps me make sure I got every spot covered. Would rather have a whitish cast then end up red with sunburn.
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Reviewed in the United States on June 17, 2025
★★★★★ 4
Tear Free and No Sunburn
Size: 0.47 Ounce (Pack of 1)
This stick is pretty small, but great for carrying with you. It does not glide very well across the skin, however, my son's eyes are very sensitive and it prevents it from gliding too far over and risking burning his eyes. Spreading with fingers afterwards is always a good idea, leaves a bit of a cast but I am very fair so not too bad, easy for reapplication as well.
The only major drawback is how drying this product is to the skin, not immediately, but over the course of several days. Not sure if that is all 100% zinc oxide products, or if this one is just lacking in something. Overall a good safe option for little faces that is tear free and wont run or burn eyes, and that works against UV.
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Reviewed in the United States on May 30, 2024
★★★★★ 5
Sun care for Babies.
Size: 0.47 Ounce (Pack of 1)
Great scent, coverage, and good on sensitive skin. My grand daughter is a blond, peaches and cream complexion, the sunblock works. No sunburned nose or cheeks this summer. We applied a swipe about every 90 min and it works for us. The little one has sensitive skin and we try to protect her completely. This product is now part of her sun care regimen. ( I even applied it to her hair part and ears)
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Reviewed in the United States on August 29, 2025
★★★★★ 5
I love sticks. Convenient and no product runs.
Size: 0.47 Ounce (Pack of 1)
Purchased for my toddlers 3 - 5 years old faces. Easy to apply. Doesn’t break out or irritate skin. Prevents sun burn if you apply
About 30 mins before water play or outdoor play in heat. Doesn’t bother their eyes. I like the sticks so I can smooth over face and cheeks with out the sun screen running. Good stuff.
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Reviewed in the United States on June 28, 2025
★★★★★ 5
Absolutely love!!
Size: 0.47 Ounce (Pack of 1)
By far the best baby sunscreen I’ve used! Better than Mustela sunscreen stick. This one glides on comfortably, is sheer and does not go on white at all, and smells great and the lid slides on and off easily. It’s quite durable and just the ultimate best sunscreen stick. The ingredients seem to be as safe and nontoxic as the other big brands and the price is very reasonable. Will be repurchasing.
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Reviewed in the United States on January 6, 2025