SKU: 32645012309

UA-Glo Luminescent Mycoplasma Detection Kit

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Description

UA-Glo Luminescent Mycoplasma Detection KitProduct Specification Synonyms Mycoplasma luciferase detection kits Stability & Storage Dry ice transport. 20 storage is protected from light, and the validity period is 12 months Background The UA Glo Mycoplasma Detection Kit is used for the qualitative detection of mycoplasma contamination in cell cultures. Over 95% of mycoplasma contamination in cell cultures is caused by Mycoplasma fermentans, M. orale, M. pirum, M. hyorhinis, M. hominis, M.

Product Specification


Synonyms Mycoplasma luciferase detection kits
Stability & Storage

Dry ice transport. -20℃ storage is protected from light, and the validity period is 12 months

Background

The UA-Glo Mycoplasma Detection Kit is used for the qualitative detection of mycoplasma contamination in cell cultures. Over 95% of mycoplasma contamination in cell cultures is caused by Mycoplasma fermentans, M. orale, M. pirum, M. hyorhinis, M. hominis, M. salivarius, M. arginine, and Acholeplasma laidlawii. Live mycoplasmas produce kinases that are not expressed by eukaryotic cells, which can convert ADP into ATP. The Mycoplasma Detection Kit provides ADP and other substrates required for mycoplasma kinases to convert ADP into ATP, and then detects the generated ATP via a luciferase luminescence method to confirm whether there is mycoplasma contamination in the cell culture. This kit can quickly detect the eight aforementioned species as well as many other common live mycoplasma strains.

Components

Size

component

10T

Mycoplasma luciferase detection reagent 0.5ml

Mycoplasma luciferase detection reagent 0.1mL

25T

Mycoplasma luciferase detection reagent 1.25ml

Mycoplasma luciferase detection reagent 0.25mL

50T

Mycoplasma luciferase detection reagent 2.5ml

Mycoplasma luciferase detection reagent 0.5mL


Protocol

1. Sample Preparation for Cell Culture Medium to be Tested

  1. Take 1 mL of cell culture medium from cells in the logarithmic growth phase.

  2. Centrifuge the cell culture medium at room temperature (200 g) for 5 minutes. Transfer approximately 800 μL of the supernatant to a new centrifuge tube, making sure not to include any cell debris at the bottom.

  3. Use the prepared cell culture medium for the mycoplasma detection with luciferase assay.

2. Luciferase Mycoplasma Detection

  1. Add 50 μL of the cell culture medium sample to a white opaque 96-well plate.

  2. Equilibrate the luciferase mycoplasma detection reagent and substrate to room temperature (22°C-25°C), and mix gently.

  3. Add 50 μL of the luciferase mycoplasma detection reagent to the 50 μL of the cell culture medium sample.

  4. Mix by gentle shaking for 10 seconds, incubate at room temperature for 5 minutes, and then read the fluorescence value (Reading 1).

  5. Add 10 μL of the luciferase mycoplasma detection substrate.

  6. Mix by gentle shaking for 10 seconds, incubate at room temperature for 10 minutes, and then read the fluorescence value (Reading 2).

3. Results
The ratio of Reading 2 to Reading 1 is used to determine if mycoplasma contamination is present.

 

The ratio of reading 2 to reading 1

Results Explanation and Recommendations

< 1

No Mycoplasma contamination

1-1.2

Possible Mycoplasma contamination. The cells were isolated and tested again after culture for 24-48hr.

> 1.2

Mycoplasma contamination



Guidelines

  • Aliquot Storage: Aliquot and store the reagents according to the instructions at -20°C to ensure reagent stability.

  • Freeze-Thaw Stability: Reagents stored at -20°C, protected from light, can be freeze-thawed twice without affecting the test results.

  • Sample Collection: Collect samples from cells in the logarithmic growth phase for testing. Newly passaged cells should be tested 24-48 hours after subculture.

  • Luciferase Reaction Sensitivity: The luciferase reaction is sensitive to temperature changes. Reagents and samples should be equilibrated to room temperature (22°C-25°C), and the temperature should remain constant (±1°C) throughout the test.

  • For Research Use Only: This product is intended for research purposes only.

Shipping Notes
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Exchange/Return Notes
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SKU: 32645012309

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Nicholas D. Clark
Louisville, US
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Don’t buy for restaurant industry
Size: 10.5, Color: Black, Size: 10.5, Color: Black
Good looking shoes, fairly comfortable and good value based on the description shared, so I don’t feel it’s fair to rate them lower than 4 stars. I had high hopes for them based on previous reviews from others saying they wore them in the food and beverage industry that mentioned their quality and durability, however I strongly recommend looking elsewhere if you do. I purchased these to wear working in a full service upscale restaurant and in less than two months have managed to have torn the material on the middle exterior section and the bottoms of the shoes are already completely worn down. Good design, but the durability needs to be improved if they’re to hold up for that industry.
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Damon Richards
Dallas, US
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Size: 11, Color: Black, Size: 11, Color: Black
I bought these for work as a bartender in August of 2025. By December, the tops were scuffed, frayed, and peeling beyond repair, and by February, the soles were coming off. I’ve never had a pair of shoes with such low quality.
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Daniel Lucarell
Dallas, US
★★★★★ 5
Most comfortable shoes, right out of the box!
Size: 8.5, Color: Cognac Napa Leather
Oh my! So, my ordering of shoes online has been hit or miss - 1/2 size too big or too small; too wide or not wide enough. Enter these Marc Joseph shoes… such a game changer. They fit like a glove, exceptionally comfortable, and easy to slip in and out of. I like them so much that I am shopping for another pair. So far, the size, quality, fit, feel, look, and comfort make this pair my dedicated first pick. Btw, I have been wearing them for six hours and they are still very comfortable! I highly recommend these shoes.
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Reviewed in the United States on September 28, 2025

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