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Description
Human ALPG/ALPPL2 Protein, His TagProduct Specification Species Human Synonyms Alkaline phosphatase, germ cell type, ALP 1, Alkaline phosphatase Nagao isozyme, Alkaline phosphatase, placental like, Germ cell alkaline phosphatase (GCAP), Placental alkaline phosphatase like (PLAP like), ALPPL, ALPPL2 Accession P10696 Amino Acid Sequence Protein sequence (P10696, Ile20 Asp503, with C His Tag)
Product Specification
| Species | Human |
| Synonyms | Alkaline phosphatase, germ cell type, ALP-1, Alkaline phosphatase Nagao isozyme, Alkaline phosphatase, placental-like, Germ cell alkaline phosphatase (GCAP), Placental alkaline phosphatase-like (PLAP-like), ALPPL, ALPPL2 |
| Accession | P10696 |
| Amino Acid Sequence | Protein sequence (P10696, Ile20-Asp503, with C-His Tag) IIPVEEENPDFWNRQAAEALGAAKKLQPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPETFLAMDRFPYVALSKTYSVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTRVQHASPAGAYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKHQGARYVWNRTELLQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALLLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDGETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPRAGTTD |
| Expression System | HEK293 |
| Molecular Weight | Predicted MW: 54.3 kDa Observed MW: 65 kDa |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4 with 3% trehalose. |
| Reconstitution | Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation. |
| Stability & Storage | 12 months from date of receipt, -20 to -70 °C as supplied. |
Background
Alkaline Phosphatase, Germ Cell (GCAP) is a tissue-specific isoenzyme of alkaline phosphatase. It is primarily expressed in primordial germ cells and the placenta, making it a classic immunohistochemical marker for germ cell tumors (GCTs), such as seminomas and dysgerminomas. Its high expression in these malignancies aids in distinguishing GCTs from other cancer types. While its precise physiological role remains unclear, it is believed to be involved in cell migration and differentiation during early development. Clinically, measuring GCAP levels is valuable for diagnosing, staging, and monitoring treatment response in patients with germ cell-derived cancers.
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