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Description
Human WNT2B ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Wnt-2b (WNT2B). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Wnt-2b (WNT2B) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Protein Wnt-2b ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Wingless-type MMTV integration site family member 2B (WNT2B), also known as WNT13, is a protein encoded by the WNT2B gene. This gene encodes a member of the wingless-type MMTV integration site (WNT) family of highly conserved secreted signaling factors. WNT family members play a role in various developmental processes, including the regulation of cell growth and differentiation, and are characterized by a WNT core domain. This gene may play a role in development and carcinogenesis. It produces two alternative transcript variants. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.3 ★★★★★
Based on 5 reviews
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Product Reviews
★★★★★ 5
Great for Sensitive Skin
Color: Non-Tinted, Size: 1.7 Fl Oz (Pack of 1)
My face has always been sensitive to sunscreens, so I have to be careful with what I use on my face. (Others can cause burning sensations). This sunscreen is great. I had no issues, and unlike other sunscreens, sweat running didn't cause my eyes to burn or water which was great.
Keeps my skin protected against the hot Florida sun, so it works!
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Reviewed in the United States on May 31, 2026
★★★★★ 3
And the crowd goes mild
Color: Tinted, Size: 1.7 Fl Oz (Pack of 1)
I had heard such great things but I’m really left a little disappointed. I got the light tinted version, and it’s much darker than anticipated. And the smell is not great. It kind of smells like my grandpa’s trailer and not in a nice, nostalgic way. As a sunscreen though, I have no complaints. The protection is great and at the end of the day that’s what I really need out of this.
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Reviewed in the United States on June 2, 2026
★★★★★ 5
Gentle but effective
Size: 4 Fl Oz
I’ve used quite a few exfoliating products over the years, but I keep coming back to this one because blackheads seem determined to make themselves at home in my pores. No matter how consistent I am with the rest of my skincare routine, they always find a way to show up, especially around my nose and chin.
What I like most about this exfoliant is that it works without feeling harsh. It has a lightweight liquid texture that applies easily and absorbs quickly. Unlike physical scrubs that can leave my skin feeling irritated or over-exfoliated, this provides gentle chemical exfoliation with none of the rough scrubbing. My skin feels smoother after use, and I don’t get the tight, stripped feeling that some acne-focused products can cause.
After using it regularly, I notice fewer clogged pores and a visible reduction in blackheads. My pores also look less noticeable, and my skin texture appears more even overall. It helps keep congestion under control before it turns into larger blemishes, which is a big reason why it has remained a staple in my routine.
For me, this is one of those rare products that manages to be both effective and gentle at the same time. If blackheads and clogged pores are an ongoing battle, this has been one of the most reliable products I’ve found for keeping them under control while still being comfortable enough for regular use.
Summary: This exfoliant has earned a permanent spot in my skincare routine. It helps reduce blackheads, keeps my pores looking clearer, and improves overall skin texture without irritating my skin. For anyone dealing with persistent pore congestion, it’s easy to see why this product has such a loyal following.
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Reviewed in the United States on June 4, 2026
★★★★★ 5
If you actually understand how to use it, it's amazing.
Size: 4 Fl Oz
My skin type:
- Very Dry, easily dehydrated
- Oily nose
- Prone to stubborn acne and closed comedones
- Redness on chin and cheeks (not rosacea)
I already use a nightly Tretinoin cream (.04% concentration) to help with my acne and premature signs of aging but decided with my derm that incorporating a BHA would be a good idea to help with my seriously stubborn closed comedones and some irritating textural issues. It was between this and the Cerave SA lotion and I ultimately decided on this due to the higher concentration of salicylic acid.
It's moisturizing, and feels sticky for the first 5-10 minutes after application but seems to sinks in to something of a satin finish on me and I can no longer feel it on my skin. You just have to be a little patient. My skin feels well hydrated and plump after it settles and looks really smooth and my pores aren't as obvious. It sits just fine under makeup for me but I'm not a new user to chemical exfoliants so I don't have lots of dead skin pilling up or sloughing off when I use it, my skin is, for the most part, already very smooth outside the acne and CCs. Occasionally I'll apply it further down my neck/decolletage than usual and WOW do I old skin just ball up and peel right away - it's so gross but also satisfying. So those that don't exfoliate often or are new to BHA/AHAs will likely have issues with foundation until their skin adjusts (but it will adjust). My advice in those cases is to either use this at night for the first few weeks or just accept going barefaced for a little while if you can.
I'd strongly suggest not applying this product over anything that you feel does not dry down fully, the times I've attempted to use it after serums or heavier products it takes forever to sink in and sometimes never fully does. I save all my serums and essences for after this has absorbed and it's far easier to work with it as a result. My steps go like this most days: ACWell Licorice toner --- PC 2% BHA --- 10% Niacinamide serum --- TO Matryxil serum --- Secret Key Black Snail cream or Syn-ake cream w/ 3 drops Stratia Fortify oil --- Sunscreen.
It works fairly quickly IMO, on my neck where I typically don't apply my Tret at night I noticed the peeling and balling of dead skin on day one and on my face where I have a patch of really awful CCs I noticed them getting smaller and smoother by around day 3-4. I'm going on about 8 days now and they're barely visible unless I'm right up in the mirror and feel as if they're almost gone when I touch my fingers to that area. I do have some slight peeling of the skin around my mouth and nose but it's not terrible (likely because I already exfoliate the heck out of my skin with the tret).
Some tips and things keep in mind:
1)
DO NOT RINSE IT OFF. I don't understand some of the top reviews advising people to do that, it says on the bottle not to rinse and nearly all chemical exfoliators are meant to be left on until the next cleansing specifically so they can do their job. If you rinse it off you're either going to prevent it from properly exfoliating or end up irritating the hell out of your skin. If you have oily skin and feel this might be heavy on you than you can probably just use this on bare skin and then sunscreen if you use in the AM or a lightweight lotion/cream and be done with it, but you need to leave it on your skin.
2)
You have got to use sunscreen while you're using this product! Any BHA or AHA exfoliant causes increased sun sensitivity and you have to address that in your routine. You should already use sunscreen daily but it becomes a must when you incorporate a chemical exfoliator or you risk sun damage.
3)
Less is more! Don't slop this on your face or soak a cotton pad in it, a little goes a long way and you'll be kicking yourself if you apply way too much and have to wait 20+ minutes for it to sink in. Use a shake or two and add more if needed but go easy.
4)
Expect some irritation initially. Chemical exfoliants can be harsh and rough even on sturdy skin and you have to give yourself time to adjust. Over-exfoliation is a possibility but sometimes just introducing a BHA or AHA into your routine can cause some sensitivity. Don't panic and just be gentle to your skin. Use ceramides (like Cerave Skin Renewing cream) or fatty oils to soothe your face if needed and try to stay away from any harsh products or acids like Vitamin C right after applying this to avoid really burning your face.
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Reviewed in the United States on January 9, 2020
★★★★★ 5
Amazing exfoliating toner
Size: 4 Fl Oz
I am almost 40 and have had acne since puberty. I have tried so many products. The products that showed some improvement (like Proactiv) changed the texture of my face. Literally made my face feel like a basketball. I’ve tried some products that I’ll notice an improvement after a week or so, and the it would just stop working. I have never used something that so drastically helped my face in such a short amount of time. Notably, within 5 days my blemishes were almost gone. Within a week, my chin felt smooth instead of feeling the bumps of every clogged pore. The discoloration of my face, such as the redness, hasn’t totally disappeared, but it is improving. My face doesn’t feel oily throughout the day. My skin feels tighter, like I got a facelift. Moisturizer along with this product is important. And it is important to follow the directions and start slowly with this product. Use it every other day for the first week, gradually working your way up to daily and then twice a day. It is a shock to your skin and can dry it out if you go overboard. But for what I needed this product for, it has exceeded my expectations in every way. I couldn’t recommend it more. Just a heads up, it is applied like a toner. I expected a face wash, but it is a liquid that you apply to cotton and rub on your face. I wash with CeraVe SA cleanser, follow it with this, and apply whatever moisturizer you prefer—I’ve been using moisturizer with SPF so as to not damage my skin more than I already have.
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Reviewed in the United States on May 12, 2026