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Description
Mouse TRPV1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Transient Receptor Potential Cation Channel Subfamily V, Member 1 (TRPV1). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Transient Receptor Potential Cation Channel Subfamily V, Member 1 (TRPV1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Transient Receptor Potential Cation Channel Subfamily V, Member 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Transforming receptor potential cation channel subfamily V, member 1 (TRPV1), also known as capsaicin receptor and vanilloid receptor 1, is a protein encoded by the TRPV1 gene. It is the first member of the transient receptor potential vanilloid receptor protein group to be isolated, a subfamily of proteins. Its function is to detect and regulate body temperature. Additionally, TRPV1 provides the sensation of burns and pain (nociception). In primary afferent sensory neurons, it collaborates with TRPA1, a chemical irritant receptor, to mediate the detection of noxious environmental stimuli. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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Exchange/Return Notes
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4.9 ★★★★★
Based on 8 reviews
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Product Reviews
★★★★★ 5
Stress and worry free with asurions complete protect plan
Absolutely thrilled with asurions complete protect plan. Worth every penny. My son is on the spectrum, and is very rough with devices, and I myself, am clumsy and go through devices more than the average person. Asurions comply protect plan, holds true and does exactly as promised. It feels so good to know, that next time a device breaks, I don't have to worry and stress over affording a replacement. Claims take literally 5 minutes to file, and I've not yet had an issue with anything not being covered. The only problem I ever had, was initially finding out how to contact them and file a claim. Which is simple. Don't contact Amazon, go to asurions direct website and follow the prompts. I highly recommend asurions complete protect plan, if you like removing stress from your life, and feeling a bit of security.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 18, 2026
★★★★★ 1
Worst company I’ve ever dealt with
This is probably the strongest version to use publicly because it stays factual, detailed, and angry without sounding unhinged. That’s what usually hits hardest with companies like Asurion because it reads like a documented timeline instead of just rage.
I have never dealt with a more disorganized and concerning claims process in my life than what I experienced with Asurion regarding my DJI Mavic Mini Pro 5 drone claim.
This entire nightmare started before the claim was even filed. For over TWO DAYS, multiple Asurion representatives insisted my product “wasn’t registered” and refused to process my claim. Meanwhile, Asurion’s own phone system literally states: “Great news, you no longer have to register your products.” Their own employees did not understand how their own system worked. I was transferred endlessly between departments, hung up on repeatedly, and even sent to Amazon support despite Amazon having nothing to do with the issue. It finally took speaking to a supervisor on the THIRD DAY for someone to admit they were wrong and manually file the claim.
I mailed my drone on May 4th regarding a serious GPS/compass issue where the drone would unexpectedly drift or take off on its own while recording. Considering this is a nearly $2,000 flying device, this was obviously a major safety concern.
Then things somehow got worse.
On May 5th — less than 24 hours after receiving the drone — I got an email saying it had already shipped back to me. No repair details. No explanation. Nothing.
When I called asking what repairs were actually performed, supervisor “CES” told me there were “no notes” explaining what had been done. She promised she would investigate personally and call me back within 24 hours.
She never called back.
On May 8th, after following up myself, another supervisor finally stated that the technician had “soldered/re-soldered” something on the drone. No explanation of WHAT was soldered. No detailed repair notes. No documentation. Nothing.
This is a drone that had ZERO physical damage and had NEVER been crashed.
I asked for written documentation of the repairs and was refused. I was told to “check the portal,” even though the portal contained absolutely no repair information whatsoever. Another supervisor apologized and promised to email me detailed information immediately.
That never happened either.
At this point, multiple supervisors had promised callbacks, documentation, and escalation reviews — and not a single one followed through.
The drone arrived back while I was still on the phone with Asurion. I powered it on and immediately received compass calibration errors. The drone would not properly calibrate.
So after all this, the drone STILL had issues.
Instead of taking accountability, supervisor “Ryan” accused me of “chasing a reimbursement check,” which is insane considering Asurion themselves only offer two resolutions: repair or reimbursement. He repeatedly insisted that because I “received a service,” the issue was basically over. He refused to acknowledge my concerns regarding undocumented soldering repairs on a nearly $2,000 aircraft and ultimately hung up on me.
Since then, the situation has only become more absurd.
Some supervisors told me the situation was unacceptable and assured me I would likely be reimbursed if I mailed the drone back. Others told me they needed more photos, receipts, and serial numbers — despite the fact that all of this had already been submitted previously and somehow “disappeared” from their system.
One representative demanded photos of a serial number physically located on the drone itself. I explained that DJI stores the serial information digitally through the controller/settings, something Asurion should absolutely know if they repair DJI drones professionally.
That representative then refused to help me further and hung up.
Days later, another representative suddenly approved a return shipping label anyway, making all the previous arguments and delays completely pointless.
Now, after weeks of this chaos, I’ve been told by another supervisor not to even send the drone back yet because they “aren’t sure what they’re going to do.” I was explicitly told they may not repair it and may not reimburse it either.
So to summarize:
* Asurion employees didn’t understand their own registration system.
* I was repeatedly hung up on and transferred around.
* Multiple supervisors promised callbacks and never followed through.
* My nearly $2,000 drone was “repaired” in under 24 hours with vague undocumented soldering work.
* Nobody can tell me exactly what was repaired.
* The drone still had calibration issues immediately after return.
* Supervisors contradicted each other constantly.
* Previously submitted documents mysteriously disappeared.
* I was accused of “chasing a check” for wanting accountability regarding a failed repair.
* I still don’t know whether they plan to repair, reimburse, or do nothing.
This has been one of the most frustrating, unprofessional, and concerning customer service experiences I have ever dealt with.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 13, 2026
★★★★★ 5
Love it.
Color: Yellow
I bought this because it works great with my DeWalt battery.
My driveway was overgrown and it locked up a couple of times which I'm assuming is a safety feature. Once I got the driveway cleaned up it works just fine.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 25, 2026
★★★★★ 5
My favorite E edger so far.
Color: Yellow
For a battery powered unit, it was much better than I expected. Powerful enough to do everything in my yard. It beats even some of my previous good gas ones.
I did my whole large front yard with a little more than one battery. Pretty good, actually.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 5, 2026
★★★★★ 4
Does a good job, and works pretty well. Took weeks to have it delivered.
Works pretty well, seems to be slightly underpowered. I think it would work better if the soil here were drier. The shroud around the blade would probably not jam up with dirt if the soil here is drier. I was constantly having to remove the battery and using a screwdriver to remove the dirt from the shroud. The shroud would work better if it was larger. There is an adjuster that lowers a metal guide that fits up against the concrete that helps you to keep the blade right up to the edge, make sure to lower that guide into place. The 20 Volt Max batteries I used are hard to insert and remove, but mine are aftermarket batteries, and another tool I own is also hard to insert and remove the same batteries.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 18, 2025
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