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Description
Mouse TL ELISA KitProduct Specification Sensitivity 9. 375ng ml Species Reactivity Mouse Theory This kit uses competitive ELISA assay, and the experimental duration is 2 hours. The plates supplied in the kit have been pre coated with TC. The TC to be tested in the sample or standard will compete with the TC previously coated on the microplate for binding to a fixed amount of biotin labeled anti TC antibody. Free components are washed away. HRP streptavidin (SABC) is
Product Specification
| Sensitivity | 9.375ng/ml | ||||||||||||||||||||||||||||||||||
| Species Reactivity | Mouse | ||||||||||||||||||||||||||||||||||
| Theory | This kit uses competitive ELISA assay, and the experimental duration is 2 hours. The plates supplied in the kit have been pre-coated with TC. The TC to be tested in the sample or standard will compete with the TC previously coated on the microplate for binding to a fixed amount of biotin-labeled anti-TC antibody. Free components are washed away. HRP-streptavidin (SABC) is added, and biotin specifically binds to streptavidin to form an immune complex. The unbound conjugate was washed off and TMB chromogenic substrate was added. The TMB became blue under the catalysis of horseradish peroxidase (HRP) and turned yellow after the reaction stop solution was added. The OD value was measured at 450nm with a microplate reader. Using curve equation software, the OD450 value of the sample was compared to the standard curve to determine the concentration of TC in the sample. There is an inverse proportion between the concentration of the target substance and the OD450 value. | ||||||||||||||||||||||||||||||||||
| Synonym | Mouse TC (Total cholesterol) ELISA Kit | ||||||||||||||||||||||||||||||||||
| Detection Type | Competition Law | ||||||||||||||||||||||||||||||||||
| Composition |
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| General Notes | 1. When using different kits, it is necessary to mark them first to prevent the components from being mixed, resulting in experimental failure. 2. After the kit is opened, please refer to the component storage conditions table for the storage conditions of the enzyme plate and standard product (the activity will decrease after moisture). 3. Please use a sterile disposable tip to suck the reagent. After use, the reagent bottle cap must be tightened to prevent microbial contamination and evaporation. 4. When washing the plate manually, add the tip or dropper of the washing liquid and do not touch the hole of the enzyme labeled plate. Inadequate washing or contamination tend to cause false positives and high backgrounds. 5. During the testing process, please prepare the reagents required for the next experiment in advance, and add the reagents to the plate holes in time after washing the plate to prevent the plate holes from drying out and causing testing failure. 6. Do not use reagents from other batches of kits or reagents from other sources for this kit without confirmation. 7. Do not reuse disposable tips to avoid cross-contamination. 8. After the sample addition is completed, the film is applied to prevent the sample from evaporating during the incubation process, and the incubation process is completed at the recommended temperature. 9. Please wear laboratory coats, masks, gloves, etc. during the test, and do a good job of protection. Especially when testing blood or other body fluid samples, please follow the national biological laboratory safety protection regulations. |
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| Instructions | Serum, plasma, cell culture supernatant, cell lysate and other fluid samples | ||||||||||||||||||||||||||||||||||
| Storage Temp. | Unopened kit, stored at 2-8 °C, shelf life for one year. | ||||||||||||||||||||||||||||||||||
| Test Range | 15.625-1000ng/ml |
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4.8 ★★★★★
Based on 19 reviews
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Product Reviews
★★★★★ 5
Good book
Format: Hardcover
Spent a year living in Ethiopia 2004 to 2005. What is have read seems very accurate. Very informative book on the history of Ethiopia.
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Reviewed in the United States on April 18, 2026
★★★★★ 5
Ethiopia from the time when the Queen of Sheba ruled
Format: Kindle
Ethiopia has a long rich history that Captivating History managed to cover in 100 pages. This book starts out by letting us know that Ethiopia is one of the first places humans settled in. One of the first kingdoms in Ethiopia was the Kingdom of Aksum. Like most African countries, Aksum established trade routes and had the silk road running through their kingdom.
The Queen of Sheba is a controversial historical leader. Many scholars believe she was queen in Ethiopia. The bible states that a queen of the south came to Israel to meet King Solomon. Historians believe that the queen of the south was in fact the Queen of Sheba. While the queen was there her and King Solomon became very close and when the Queen of Sheba returned to Ethiopia she had King Solomon's baby. Menelik I may have been King Solomon's son. Some believe that when Menelik I when to visit his father, King Solomon, Menelik returned to Ethiopia with the Ark of the Covenant. Some believe that the ark is hidden away in some Ethiopian monastery, still today.
There is still a lot of Ethiopia's history left for you to learn about by reading this book.
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Reviewed in the United States on August 15, 2022
★★★★★ 5
History of Ethiopia
Format: Kindle
Ethiopia is an essential nation-state in Africa. It has a great civilization and history. The reign of Johannes IV was crucial to the Kingdom of Ethiopia. Moreover, Haile Selassie was another vital emperor. Ethiopia suffered under communism. Currently, Ethiopia faces foreign challenges from its neighbors and political corruption domestically. Specifically, political unrest dominates the domestic life of Ethiopia. The author presents and discusses the achievements and challenges of the Ethiopian people from Ancient Times to Modern Times. It is a well-written book.
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Reviewed in the United States on August 29, 2022
★★★★★ 5
While traveling in Ethiopia as a tourist.
Format: Kindle
It is a very concrete and well written history of Ethiopia. While traveling in a photo tour I decided I wanted to know more of the contrasting country my shoes were on. I think this short book completes my need to my entire satisfaction
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Reviewed in the United States on March 9, 2023
★★★★★ 4
Informative, compact history of rulers, religion and struggles.
Format: Kindle
This book is well written. It filled out my knowledge of Ethiopia, which was mostly that it was isolated from neighbors and most empires. I had heard of biblical contacts, two Italian invasions, civil wars and famines.
In this book I learned of contacts with ancient Rome, Byzantium, the Ottomans, and Arabs. Trade carried on the silk road, and by sea. There was influence of Judaism, Islam and competing Christian sects. The sequence of leaders were covered as well as how they came to power. Many conflicts are covered, insurrections and invasions. The Haile Selassie period is covered, as well as his downfall. Then the military period is covered, with communism and factionalism. Finally the recent period is covered,up to the present civil wars of 2022.
I would recommend this compact book, for anyone planning a visit, or wanting background knowledge.
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Reviewed in the United States on September 10, 2022