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Description
Rat SDC4 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000 × g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash the collected cells three times with ice-cold PBS and resuspend them in 150-200 μL of PBS per 1 × 10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500 × g for 10 minutes at 2-8°C, and collect the supernatant for analysis. 5. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Preparation of standard gradient working solution: Add 1mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 2000pg/mL). Then dilute to the following concentrations: 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, and 0pg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each tube. Pipette 500uL of the 2000pg/mL standard working solution into the first EP tube and mix thoroughly to make a 1000pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube is used as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Syndecan 4 (SDC4) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Syndecan 4 (SDC4) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Syndecan 4 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Syndecan-4 (SDC4) is a protein encoded by the SDC4 gene. It is one of four vertebrate syndecans and has a molecular weight of approximately 20 kDa. It activates protein kinase C (PKC), an enzyme involved in signal transduction. Its variable domain may function as a self-binding site. The degree of oligomerization correlates with kinase activity, and thus the degree of syndecan-4 aggregation correlates with PKC activity. Syndecan-4 also binds to phosphatidylinositol (4,5)-bisphosphate (PIP2) via its variable domain, increasing PKC activity by 10-fold. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 31.2-2000 pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates, cell lysates and other biological fluids |
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4.2 ★★★★★
Based on 19 reviews
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Product Reviews
★★★★★ 5
Comprehensive! The Bible of Deep Learning!
This book has by far surpassed my expectations! I have purchased many machine learning and deep neural network books in the past, but nothing has ever come close to this book! First of all, it is written by the fathers of Deep Learning, and is therefore an authority. Secondly, the book is broken into three parts: 1. A math overview and refresher. 2. Deep Learning applications and 3. Research in Deep Learning. I can't help but go through this book from front to back. It is a smooth read, and every sentence written is meaningful. These guys know their stuff! And after you read this book, YOU WILL ALSO know your stuff!
If you feel daunted by the price, just remember, you get what you pay for! I'd say they could easily charge about $300+ for this book, but they are doing everyone a very kind favor by ONLY charging this reasonable amount. You get A LOT of bang for your buck with this purchase. I hesitated at first about buying this book because of the price, but I am soooooo happy that I did! Worth every penny! Look no further, get this book and start your Deep Learning journey!!
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Reviewed in the United States on July 14, 2017
★★★★★ 1
A rushed, poorly written guide of how the "experts" can't really explain what Deep Learning is
Format: Hardcover
This book, in every sense of the word, is rushed. I think the authors wanted to establish themselves as leaders of this young-ish field, but does so by sacrificing quality. It also shows that Deep Learning theory has been there for a long time, known by another name called Neural Networks. The interesting algorithms are of MLP, Back Propagation and the classical neural networks. The optimization methods such as Adam are the ones that are new and interesting, and the only ones worthy of in this book. So, essentially, what you get from this book is use A for X, B for Y and C for Z type of dry, un-intuitive, badly written waste of paper.
As for the structure of the book, it's like an example of how not to structure a book. It has some linear algebra, probability at the start (not good enough, and confuses more people and wastes paper). Goes on to prove other algorithms such as PCA (yeah, ok!). Then, talks about how this architecture works for this and that architecture.
So, yeah, if you really want to try out deep learning, don't buy this book. Set up Tensorflow/pytorch/ other library, run the tutorials, find an architecture for the problem you are interested in and start tweaking that. You will have far more fun and would have saved your money.
The praise that this book gets is beyond me. Did Musk even read this book? I doubt it.
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Reviewed in the United States on May 15, 2018
★★★★★ 5
The classic textbook on Deep Learning
Format: Hardcover
Deep Learning is the promising direction towards general purpose effective artificial intelligence.
There is an explosion of fruitful research in recent years and a lot of applications pursued mainly from technology giants as Google, Amazon, etc. and outstanding research institutions.
The book "Deep Learning " by Ian Goodfellow, Yoshua Bengio, Aaron Gourville, is an excellent piece of work.
They manage to present rather difficult things in an understandable manner. The theoretical presentation is outstanding typical of "classic" books. Also, the book stays close to the practical applicability of all the methods and discusses applications extensively.
There are a lot of other useful books on deep learning that follow a more practical approach by focusing on a particular deep learning software package, but this one book is certainly much more essential since it provides the required theoretical background in order to be able to do serious work on deep learning.
I consider the book as "must have" for anyone that works on deep learning either in an academic or in an industrial environment.
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Reviewed in the United States on August 25, 2018
★★★★★ 5
100% Recommend
Format: Paperback
Invincible Compendium One completely lived up to the hype. From the very first chapter, I was hooked by the story, the action, and the character development. What starts off feeling like a classic superhero story quickly becomes something much deeper, darker, and way more emotional than expected.
The artwork is incredible and the fight scenes are intense without feeling repetitive. Every character feels important and layered, especially Mark and Omni-Man. The pacing is excellent for such a massive collection, and it’s hard to put down once you start reading.
If you’re a fan of superhero comics but want something with real stakes, shocking twists, and strong storytelling, this is absolutely worth reading. Easily one of the best graphic novels I’ve picked up in a long time.
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Reviewed in the United States on May 23, 2026
★★★★★ 5
This is the best purchase I’ve made in 2025!
Format: Paperback, Format: Paperback
I don’t even know where to start ever since I finished (in early August, 2025) the whole series of this comic. I have never been this much speechless in my life. It’s my first comic that I’ve ever read and finished it.
I got into invincible through the TV show and I wanted to give a shot on comics because people said it’s even better than the show, and I was blown away just by the tv show until I started reading this.
It didn’t matter how tired I would be and/or how busy I would be, but I’d still make time to grab and read at least some pages very excited.
There are so many parts in this comics where it reflected on my personal life and somewhat opened my eyes of what I had and need to do in my life. It was really fun, exciting, loving, caring, sad and emotional story. This comic has a definitive beginning and a final, conclusive ending.
I loved it overall despite where there were some boring parts, but it all made sense later in the main story. Very well developed characters, stories and art.
Now whenever I pass by my bookshelf and I see these books puts big fat huge smile on my face. I’m glad I have it in my collection. I got to experience in this comic what I couldn’t in real life.
Every other night in random or toughest moment I would think about what would mark do in this situation.
Thank you for this masterpiece ❤️
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Reviewed in the United States on September 18, 2025