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Description
Cytochrome C Recombinant Rabbit mAb (S-2422-56)Product Specification Host Rabbit Antigen Cytochrome C Synonyms Cytochrome c; CYC; CYCS Immunogen Synthetic Peptide Location Mitochondrion Accession P99999 Clone Number S 2422 56 Antibody Type Recombinant mAb Isotype IgG Application WB, IHC P Reactivity Hu, Ms, Rt, Mk Positive Sample HeLa, HepG2, Molt 4, SH SY5Y, C2C12, mouse brain, mouse kidney, C6, rat brain, rat kidney, COS 7 Predicted Reactivity Bv, Cz, Dg, Hr, RhMk Purification Protein A
Product Specification
| Host | Rabbit |
| Antigen | Cytochrome C |
| Synonyms | Cytochrome c; CYC; CYCS |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion |
| Accession | P99999 |
| Clone Number | S-2422-56 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, HepG2, Molt-4, SH-SY5Y, C2C12, mouse brain, mouse kidney, C6, rat brain, rat kidney, COS-7 |
| Predicted Reactivity | Bv, Cz, Dg, Hr, RhMk |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:10000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:2000 | Hu, Ms, Rt |
Background
Cytochrome c is a small, highly conserved heme-containing electron-transfer protein located in the intermembrane space of mitochondria, where it shuttles electrons between Complex III and Complex IV of the respiratory chain via reversible Fe²⁺/Fe³⁺ redox changes in its covalently attached heme group, and, beyond its bioenergetic role, its release into the cytosol in response to apoptotic stimuli nucleates the apoptosome by binding Apaf-1, making it a central switch between life-sustaining respiration and programmed cell death.
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4.5 ★★★★★
Based on 13 reviews
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Product Reviews
★★★★★ 5
Great toy for two big German Shepherds
Color: Brown
We only buy Tuffy dog toys now because we have one German Shepherd, and we often watch our daughter's GSD too, and the two dogs tear anything else apart in minutes. Tuffy toys are BY FAR superior to any other dog toy we have tried.
That being said, some Tuffy toys seem to last longer than others with the two dogs, and this one has been pretty good! We've had it for a few weeks now, and it's just now starting to show some white (stuffing) where their teeth have pierced the top of the toy.
That's pretty good, considering they play tug of war with it and can tear into toys pretty hard. If it were just our one dog, I think it would last a lot longer because it's the tug of war between the two of them that does them in faster.
Even so, when they do manage to tear the fabric in vulnerable places, I just pull the stuffing out from that area, and they continue to play with it, sometimes for months!
This one is better than some other Tuffy toys too because it has lots of areas where there is already more fabric and reinforced seams than stuffing, like all the legs on the side, the tail, and even the pincers. So far, they haven't been able to tear into those at all. Just a bit on the top of the head, and so far, no stuffing coming out yet.
This one is a big win for us! When they finally do do it in, I will turn around and buy another Scorch the Scorpion right away!
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Reviewed in the United States on May 5, 2014
★★★★★ 4
Tuffy Desert Phrog vs Lola
Color: Green Leaping Phrog, Color: Green Leaping Phrog
Just a little background about my dog. Lola is a 5 year old corgi-terrier mix with jaws of steal. Lesser stuffed toys have met death in under 5 minutes. Tennis balls last less than a day. Rope toys that do not have frayed ends might possibly last a week (frayed ends never stand a chance). The only toys that she has not managed to destroy are her Kong, a Kong bone, and a racquet ball. I figured I would document the life span of Phrog (Tuffy Scale: 9) before making my final judgement on it’s durability.
Day 1 - Phrog Arrives! Lola was very excited about the arrival of Phrog and immediately went into destruction mode. Phrog did sustain a little damage before we were able to bribe her to drop Phrog and get her understand that he was more fun to play fetch with than to mutilate. The white you see in the picture is not stuffing, but the second layer of defense for the toy. The fact that my living room is not full of stuffing and I didn’t have to pry a squeaker out of my dog’s mouth, I’d say Phrog wins the Day 1 challenge.
Day 9 - Phrog has sustained a little more damage, but not anything life threatening. His side wound is a little bit larger and one of his eyes is half ripped off. For safety reasons, I am going to finish removing the damaged eye and some loose thread from various places. Other than that, Phrog is still holding up and holds top toy spot. I have also found him very useful for dealing with anxiety; the squeaker can often distract her from her stressors and Phrog takes the abuse of her frustrations.
Day 16 - Phrog is starting to show signs of mortal wounds. Lola has made it through the thicker layer around his arm and I can see the stuffing. It is only a matter of time before gets all the way through.
Day 60 - Phrog still lives!!! I have done some surgery on Phrog to reinforce the fabric around his arms, but Franken-Phrog is still the favorite of all of the toys. She will even get brave enough to rescue Phrog from the evil vacuum when he is kidnapped and tortured. I am thinking about purchasing Phrog II for the inevitable day when Phrog goes off to the pet toyland in the sky, but for now, there is only one Phrog for Lola.
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Reviewed in the United States on July 6, 2018
★★★★★ 5
Super tough
Color: Red Warthog
My dogs love these toys!!!
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Reviewed in the United States on February 10, 2026
★★★★★ 5
Long lasting play toy!
Color: Multicolor
Tuffy toys in general are great and they are a pretty good deal on Amazon. They are not indestructible, and if your dogs use them as chew toys they will eventually break through. My dogs have had this Armadillo since Christmas (and they do chew on it some) and the only thing missing is an ear. They like to play tug-of-war with it a lot. This is a bigger toy, but even my forty pound dog plays with it. I would not recommend this toy for dogs smaller than Border Collie size. You can make these Tuffy toys last longer if you don't allow your dogs to chew them--when your dogs starts chewing on non-chew toys trade them for a chew toy and when you catch your dog chewing on a chew-approved toy reward them for chewing. Eventually your dog will catch on and realize that if they want to chew there are certain toys that are allowed for that. In addition to being a great play toy this is a pretty cute toy too!
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Reviewed in the United States on June 30, 2015
★★★★★ 1
Not Tuff
Color: Black Yellow Phrog, Color: Black Yellow Phrog
Absolutely terrible and not “tuff” at all. My chocolate lab had this thing destroyed in less than two hours. I would not recommend these for the price.
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Reviewed in the United States on April 11, 2026