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Description
Rat AQP5 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an aquaporin 5 (AQP5) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of aquaporin 5 (AQP5) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Aquaporin 5 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Aquaporin-5 is a protein encoded by the AQP5 gene. AQP5 is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin-5 plays a role in the production of saliva, tears, and pulmonary secretions. AQP0, AQP2, AQP5, and AQP6 are closely related and all map to 12q13. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
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4.8 ★★★★★
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Product Reviews
★★★★★ 5
Really comfortable, good fit
Size: 6X-Large, Color: 5 Pack(black,black,blue,blue,light Coffee)
These are very lightweight and comfortable. The material stretches freely and doesn't bind up anywhere. If you go by the size guide, they should fit perfectly neither too loose nor too tight. Would definitely recommend.
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Reviewed in the United States on April 23, 2026
★★★★★ 3
These don't work for me...
Size: Medium, Color: B005
First the good... I like the material; it's very soft, light, and stretchy. I also like the colors, but that's all I like about these boyshort panties. My main goal for panties is to have them NOT ride up into my butt crack leaving the bottoms of my butt hanging out. It's probably comfortable for some, but it drives me nuts to feel this constant need to pull them out of my butt crack and down over my butt cheeks. If I wanted my butt hanging out I'd buy thongs, LOL. The part I really don't understand, is why they make the front and the back the same width/length. I mean clearly more material would be needed in the back to fit around two round humps, right? I mean I guess unless you have what I call a pancake butt, I don't see why there shouldn't be more material on the backside of the underwear. Why is it so dang difficult to find underwear in this style to fit up over butt cheeks? If I pull them down enough to cover the bottoms of my cheeks then the waistband is sitting right at the top of my crack which is extremely annoying (then I feel this constant need to pull them up!) especially when the front is long enough to go up to my belly button!! Who are they making these underwear for??? Ladies with FLAT butts apparently. For reference, I'm 5'5" 130 lbs, hips 37", waist 27", athletic build, as in lean but with muscular glutes & quads from speed skating. Not only are they not made big enough to go up over my rump while still keeping my cheek bottoms from hanging out, but they're too long in the front cause they constantly roll up in the front. I've even tried turning them around, even though they seem to be exactly the same front to back, and they fit exactly the same, no surprise there. I may just be out of luck finding comfy boyshort panties that actually fit the way I want them to. :/
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Reviewed in the United States on December 15, 2025
★★★★★ 5
Super comfy!
Great colors, super comfy, soft and druable!!
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Reviewed in the United States on May 2, 2026
★★★★★ 5
Comfy and well made
Size: Large, Color: B2207sp
Love these! They stay in place, are soft and comfortable with just enough stretch but hold their shape. This is my 3rd order.
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Reviewed in the United States on February 21, 2026
★★★★★ 5
Super soft and comfortable!
I haven't bought new underwear in forever. I used to wear thongs, but now find them incredibly uncomfortable. The last few times I've tried to buy new underwear, the sizing was either way off (I'm a medium, but gave up after having to return them for a large and then extra large) or the quality was horrible (two brands stretch waaaay out and they can fall down while wearing them (I have larger ass-ets, so they shouldn't start to slide down during use). I tried these after buying some bamboo pyjamas and I love them! They are incredibly soft, comfortable, and stretchy. I can use them all day, and they work well, whether I'm lounging on the couch or wearing them under my spandex shorts at the gym, they don't ride up or slide down. They are both comfortable and look sexy, while providing support in all the right places. I haven't had them too long, so I can't speak on the durability, but they appear to be of good quality and I plan to order more, this time without the band.
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Reviewed in the United States on November 13, 2025