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Description
Mouse Endometrial Carcinoma Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) the tissue after sampling must be placed in the pre cooled (2 8 c) sampling bottle of tissue preservation solution e, and quickly transferred to the clean laboratory for tissue processing and cell separation, taking photos and registering information (2) prepare several Petri dishes and add 4 pre cooled primary culture buffer B for standby (3) disinfect the sampling bottle, put the tissue into the Petri dish,
Product Specification
| Usage |
1. Primary (1) the tissue after sampling must be placed in the pre cooled (2-8° c) sampling bottle of tissue preservation solution e, and quickly transferred to the clean laboratory for tissue processing and cell separation, taking photos and registering information (2) prepare several Petri dishes and add 4 ℃ pre cooled primary culture buffer B for standby (3) disinfect the sampling bottle, put the tissue into the Petri dish, wash it with primary culture buffer B for three times, and then cut the tissue into a tissue block with a volume of about 1-3mm3using ophthalmic scissors or scalpel (4) the tissue was digested with mouse endometrial primary tissue digestive solution C, and then shaken for 10-20min at 37 ℃ (observe the digestion at any time during the digestion process) (5) take a small amount of liquid and observe it under the microscope. After observing more single cells or cell clusters below 70UM under the microscope, add three times the volume of primary culture buffer B to stop digestion (6) filter with a 100um pore size screen, collect the filtrate, remove the supernatant after centrifugation at 300g for 5min, add primary culture buffer B and resuspend for centrifugation (7) Matrigel calculation: after step 6, observe the collected tissue volume, add 25 times the tissue volume of Matrigel (abs9495) to resuspend the plate (8) take a 24 well cell culture plate as an example, dispense 25ul of tissue Matrigel mixture per well for plating (operate at 4 ℃) (9) put the laid culture plate into a 37 ℃ incubator for 10-15min to form glue, and add mouse endometrial organoid medium a (restore room temperature) for culture 2. Organoid subculture (1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min (2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) (3) a: the number of organoids is insufficient or the volume is small: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4  &Nbsp&Nbsp&Nbsp b: When the number of organoids is large or the volume is large: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid passage digest D for digestion for 2-3min, add organoid passage culture buffer g to stop digestion, centrifuge for 5min to discard the mixture, add an appropriate amount of organoid passage culture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4 (4) after organoid collection, add Matrigel to resuspend, lay 25ul Matrigel per well in a 24 well cell culture plate, place in the incubator for 10-15min, and add 500ul mouse endometrial organoid culture medium a 3. Organoid cryopreservation (1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min (2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) (3) centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend, and centrifuge for 5min at 300g to discard the liquid (4) add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend. Take a 24 well cell culture plate as an example: the density is 2 wells and 1 tube is frozen, with a volume of 1.4ml per tube (5) mark the information, and after cooling down the program, transfer it to liquid nitrogen for long-term storage 4. Organoid resuscitation (1) take 10ml of organoid subculture buffer g and put it into a 15ml centrifuge tube (2) take out the frozen organoid cells from the liquid nitrogen tank and quickly put them into a 37 ℃ water bath pot for melting (3) during the water bath thawing process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely thawed in 1-2min (4) quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently blow 6-8 times with a pipette, centrifuge at 300g for 5min, and then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube. Centrifuge for 5min at 300g (5) resuspend Matrigel, lay 25ul Matrigel per well in a 24 well cell culture plate, place in an incubator for 10-15min to form a gel, and add 500ul of mouse endometrial organoid culture medium A. |
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| Description | Composition:
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| Storage Temp. | 4° C. 3months; -20° C. 1 year, see label for details |
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4.2 ★★★★★
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Product Reviews
★★★★★ 5
Love these tunics!
Color: Aprioct Boho Yg9, Size: XX-Large
I absolutely love these tunics! They are very figure flattering & so soft & comfortable to wear. The material is on the thinner side so they are great for warmer weather. I have very sensitive skin so many fabrics irritate me but this one is fabulous. I would wash on gentle & hang to dry to keep fabric looking nice. After wearing the first one, I ordered 3 more…
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Reviewed in the United States on May 12, 2026
★★★★★ 5
Pretty Tunic Top
Color: Willowfloral, Size: X-Large
Very happy with this tunic top! Long enough for me to feel good about wearing it with leggings, but not SO long it feels like a dress. Really good V-neck, not just a little notch at the top that some brands call a v-neck. I ordered the teal floral XL and was very happy with the fit and drape. I'm a size 18W with a 42D bust and a chubby waistline. I often end up in XXL or 1X tops, but this fit well as an XL. Nice feel to the material and a bargain at the price!
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Reviewed in the United States on March 26, 2026
★★★★★ 5
Very flattering in my opinion
I'm 5'6 and no hips, so I really liked that the length is a little longer and flares a little at the hips giving my chunky but not curvy figure some illusion of a girlish figure. The pattern and colors were pretty & I'd say pretty close to the ad. Great blues and a coral/peach ... not sure my camera did it justice. I like the pleat at the back ... I feel that it minimizes the bra bulge that can happen at times. I stepped out after try on and my guy said it looked great (he's a truth teller with clothes) so that made me feel better because sometimes I see what I want to see at first & later see the flaws. I do see the case instructions ... getting used to hang to dry all things lately. Feels a little more nylon-ish but not bad. Its not super thin and light where I think it's a clingy thing. Really do like this shirt
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Reviewed in the United States on March 9, 2026
★★★★★ 4
Nice, but not great fabric quality
Color: Floral Black, Size: Large
The fit was great. Colors were as shown. The fabric shows signs of wear pretty much on the second wear so durability isn’t great. The fabric is soft and light weight so perfect for spring and fall. Not sure I’d buy again as I think it will look worn out quickly.
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Reviewed in the United States on April 4, 2026
★★★★★ 5
Ding! Ding! Ding! We have a winner!
Color: Boho Multicolor, Size: XX-Large
Wow. These are really nice tops. The material is soft and very comfortable and the fit is true to size. Colors are as pictured. They look and feel like they cost a lot more!
I initially order this in 2 different patterns (Boho and Willowfloral) and I just now ordered 3 more, black, purple, and wine red. Can easily be worn all year round and can be dressed up or down. All in all, I’m very pleased with this purchase.
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Reviewed in the United States on May 31, 2026
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