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Description
Annexin V-PE/7-AAD apoptosis analysis kitProduct Specification Usage 1. Instrument parameter adjustment 1. Collect 1 106 3106The cells were washed twice by centrifugation with pre cooled PBS and the supernatant was discarded. 2. Add 500uL of Apoptosis Positive Control Solution to resuspend and incubate on ice for 30 minutes. 3. Wash by centrifugation with pre cooled PBS and discard the supernatant. 4. Add an appropriate amount of pre cooled 1 Binding Buffer to resuspend, and add the same
Product Specification
| Usage | 1. Instrument parameter adjustment 1. Collect 1 × 106-3×106The cells were washed twice by centrifugation with pre-cooled PBS and the supernatant was discarded. 2. Add 500uL of Apoptosis Positive Control Solution to resuspend and incubate on ice for 30 minutes. 3. Wash by centrifugation with pre-cooled PBS and discard the supernatant. 4. Add an appropriate amount of pre-cooled 1 × Binding Buffer to resuspend, and add the same number of untreated viable cells to mix with it. Add pre-cooled 1 × Binding Buffer to replenish to 1.5 mL, and divide it into three tubes equally, of which one tube is a blank control tube and two tubes are single dye tubes. 5. Add 5 uL of Annexin V-PE or 10 uL of 7-AAD to the single stain tube and incubate for 5 minutes at room temperature and protect from light. 6. On the flow cytometer, the voltages of FSC, SSC and fluorescence channel were adjusted with a blank tube, and under this voltage condition, the fluorescence was adjusted with a single staining tube Compensation of channels. Note:Certain processes of treating adherent cells as single cells cause cell membrane damage, resulting in false positives for Annexin V. Therefore, optimization is needed. It is recommended to treat adherent cells with enzymes that are more gentle on cells, such as Accutase. The adherent cells can be cultured on a suitable vessel, such as a sterile glass slide, and stained with suitably modified methods. However, this method has not been tested in our laboratory. 2. Sample testing 1. Induce apoptosis according to the experimental protocol. 2. Centrifuge washing with pre-cooled PBS and collect 1-10 × 105Cells include cells in the culture supernatant. Dilute 5 × Binding Buffer into 1 × working solution with double distilled water, and take 500 uL of 1 × Binding Buffer to resuspend the cells. 3. Add 5 uL Annexin V-PE and 10 uL 7-AAD per tube. 4. After gently vortex and mix well, incubate at room temperature and protect from light for 5 minutes. 5. Get on the computer for analysis. |
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| Beilstein | 0 | |||||||||||||||
| Synonym | Annexin V-PE/7-AAD Apoptosis Analysis Kit | |||||||||||||||
| Description |
Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is present in the inner lining of the plasma membrane of normal cells, but in the early stage of apoptosis, membrane asymmetry is lost and PS translocates to the cell surface. Fluorescently Labeled Annexin V Kit Components:
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| PubChem CID | 0 | |||||||||||||||
| General Notes | 1. Please read the instructions carefully before using this product. This product is for scientific research only, not diagnostic. 2. For your safety and health, please wear experimental protective clothing, gloves, masks and other necessary protective equipment. |
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| Storage Temp. | Store at 2-8 °C, do not freeze, shelf life 1 year. |
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4.3 ★★★★★
Based on 13 reviews
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Product Reviews
★★★★★ 5
MUST BUY
This was a nice addition to my Prequel collection! I just loved this product and so did my skin. This is a very light weight product that hydrates and plumps the skin. I apply it after my VitC serum and it works great under my makeup. I will be repurchasing. Such a good product at a good price!
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Reviewed in the United States on April 14, 2026
★★★★★ 5
Makes my skin feel firmer
I bought this and the Prequel SPF, my first time using this product line. Definitely impressed and in my opinion, a great price for what seems like a quality product. The serum has a lightweight texture,, absorbs quickly, and my skin looks and feels well hydrated right away. More so than when I use a regular hyaluronic acid. There is no pilling or heavy feel so easy to use with other products. I can't say whether it improves skin texture over time or what the benefits are with continued use. I've been using it consistently with the SPF for the last two weeks, so it only been a short while. But when I put it on, my skin noticeably looks more springy and feels better throughout the day. I have mature, dry skin so I do use a moisturizer over this before sun protection. I imagine that if you have oily or normal skin you might be able to use this in place of moisturizer during the day.
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Reviewed in the United States on October 27, 2025
★★★★★ 5
Plump, Soft Skin with a Smooth Finish
I’ve really been enjoying the Prequel Skin Multi‑Quench Plumping Hydration Serum. It gives my skin a soft, hydrated feel without any heaviness, and it absorbs quickly so it never interferes with the rest of my routine. One of the best parts is how smoothly my makeup applies afterward—everything blends better and looks more even. Overall, it’s a lightweight, effective serum that adds the perfect boost of hydration.
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Reviewed in the United States on December 23, 2025
★★★★★ 5
New favorite moisture serum
I am blown away by this formula - it goes on silky and absorbs into the skin, giving it a plump, hydrated glow without ANY stickiness or annoying shine. Two pumps after cleansing and toner is just right but you can also go in heavier and use in place of a moisturizer. This truly gives me lasting moisture, which is amazing for a light to mid-weight serum. Wears great with other complexion products and under makeup. The glass bottle is luxe and the pump isn't the least bit messy. Glycerin is all the rage in terms of "hot" ingredients right now - and I have to say after using this, I'm a believer. I've pretty much replaced a classic daily moisturizer with pairing this serum before Strata Lipid Gold (another holy grail) - which works great under my moisturizing Japanese sunscreen (Canmake Mermaid Skin). These steps provide a flawless, juicy base under makeup for my maturing (43-year old) skin. Can't wait to try more products in this line.
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Reviewed in the United States on September 29, 2024
★★★★★ 4
Good
Moisturizing and gel like. It pills when used with the Prequel mineral SPF.
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Reviewed in the United States on March 31, 2026