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Description
Mouse ECM1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. 3. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Preparation of standard gradient working solution: Add 1mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 3000pg/mL). Then dilute to the following concentrations: 3000pg/mL, 1500pg/mL, 750pg/mL, 375pg/mL, 187.5pg/mL, 93.75pg/mL, 46.875pg/mL, and 0pg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 3000pg/mL standard working solution into the first EP tube and mix thoroughly to make a 1500pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with extracellular matrix protein 1 (ECM1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of extracellular matrix protein 1 (ECM1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Extracellular Matrix Protein 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Extracellular matrix protein 1 is a protein that, in humans, is encoded by the ECM1 gene. The extracellular protein encoded by this gene contains a cysteine pattern characteristic of the ligand-binding "double loop" domain of the albumin family. This gene maps outside the epidermal differentiation complex (EDC), a cluster of three gene families involved in epidermal differentiation. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 46.88-3000pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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4.4 ★★★★★
Based on 15 reviews
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Product Reviews
★★★★★ 4
Expensive, but it works
The good parts:
Yup, this works with DP alt mode, single cable use works for most devices I tried (steam deck, laptop, odin mini 2) even without the hub powered through the power in on the hub, I even got it working without external power to either the hub or my small usb-c monitor. However, some devices like my phone likely didn't give enough power for it to work without another power source. On my phone it worked for some usb devices without external power, but the dp alt mode didn't do work. DP alt on my phone works just fine with external power connected to the hub. Powering it with a small but powerful power bank worked fine, but lower power power banks might not keep up especially if the monitor isn't getting it's own power. It only takes a watt or two of power for the hub itself from some quick and dirty measurements charging my laptop through the pd-in on the hub.
As for the rest of it, the three usb 3 ports means you should be enough to get by for any use case, though you may need more dongles or adapters to get the type of connections you need, but quite usable as-is for a lot of purposes.
Basically it does what it says, there really is no other hub currently that can do pd alt mode, only big docks which need power supplies.
The ok parts:
*When it isn't powered* I noticed that with a monitor already plugged in, plugging it into a device would lead to the monitor not being initialized. But plugging the hub into the device first before connecting the monitor worked. No issue when it was powered. You can use this to get display out and charge with a Nintendo switch, but if it's not powered through the pd-in it probably wont switch to TV mode and output video, not sure if that's because of power or software reasons.
This is a nitpick, but if you care about the feel or aesthetic it's noticeably bland. The case is plastic and there seems to be no weighting on the inside, it's very light, which can feel cheap but it's not like adding weights would functionally make it better, the case also has a bit of a harsh feeling where it's joined, but it does feel solid and durable enough. It's clearly function over form, just barely meets the minimum bar imo.
The bad part:
You already saw, but the price is a bit steep for a hub (at $80). I'd love to see it at 60 or even 40 instead, I hope more similar hubs pop up for competition.
In summary:
It does what it says, if you know you want this and what it is then get it, I feel like it shouldn't cost this much, but it's quite usable and for now it's the only option for a DP alt mode hub.
Minus a half star each for subpar presentation and price 4/5
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Reviewed in the United States on October 4, 2024
★★★★★ 5
Great for AR Glasses
This USB hub has been great for me! It's taken me so long to finally find a stable setup to allow using my XREAL Air glasses, peripherals, and charger at the same time. I've used this both with my phone and ROG Ally and I have not once had a problem. I even found that my ROG Ally manages to get the full supported PD of 65W, as long as I power the hub with a 100W PD charger. That said though, using a 65W PD charger is still more than adequate to charge the device while using various peripherals and a USB-C display.
Previously I used USB-C hubs with HDMI out and another device to merge the HDMI output with a USB-A connection into a single USB-C cable that I use for my AR glasses. This setup worked, but would increasingly stutter/freeze the longer I used it forcing me to constantly unplug and re-plug it in. In trying to find a solution that didn't split and merge back the display signal with USB data/power, this hub was the only one I found that wasn't thunderbolt 3/4.
I will say that I wish the cable to the host device was either longer and less rigid, or shorter with an optional extension cable. An ethernet port would have also been nice, but using up one of the USB-A ports with a USB ethernet adapter works if needed. Plus, if I wanted ethernet with it, then a dock would probably have been a better option over a hub anyway.
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Reviewed in the United States on December 29, 2023
★★★★★ 5
One of the only dongles that supports a usb-c display (alt mode) AND Power Delivery!
If you are like me and have laptop that only has a couple of usb-C ports (one of which has to be used for charging), but want to free up a port, this is the ONLY dongle I have found that does the job flawlessly AND affordably. The dongle has a usb C port. for power delivery as well as a second usb C that not only supports data, but has the necessary specs to support a usb C monitor (via DP alt mode).
This is only dongle I have come across that has the necessary hardware to support a usb C monitor for less than 100 bucks.
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Reviewed in the United States on May 13, 2024
★★★★★ 5
(Steam Deck Verified) Works like a charm on Dual QHD 2560x1440 (1440p)
This could be one of those reviews where I tell you how well this works for work Zoom Meetings and the Monthly TPS Excel reports. But my primary concern was the Steam Deck my secondary concern was being able to see more work. Luckily it worked for both.
The Steam Deck is capable of pushing to both 1440p monitors without any issues. I don’t have many AAA games, but if it helps: Hogwarts Legacy ran at full speed on auto detect (medium) graphics. No ghosting, no tearing, no lag. I tried No Man Sky for something with very high particle and blooming. Still ran like a champ. Mafia Definitive Edition runs like Estes Kefauver at a meeting of Murder Incorporated. If it runs on the Steam Deck this hub can push it… Remember my monitors are only QHD 1440p.
One laptop worked amazingly. 1440p Zoom Calls, Excel Spreadsheets as far as the eyes could see. BOTH EMAIL AND CHAT OPEN ON THE SAME SCREEN!
*Car salesman roof slap*
This baby has so many pixels.
The other laptop didn’t support video over USB-C nor Power Delivery. The USB hub worked very well as a USB hub though.
The USB ports are all fast and reliable. My USB 3.x devices have varying speeds. I don’t think any device measured the full through put, but given the equipment I have to work with (it could be the equipments fault) I am happy with a 4.8 out of 5.
Using the power delivery port on this hub will allow you to charge attached devices. Steam Deck charges, Laptops with USB-C Power Delivery charge. Laptops without power delivery will not charge.
The ports with pictures of keyboards, please plug your USB Keyboard and Mouse in these ports. These ports are USB 2.0. They will run slow if any other devices are plugged into them.
If your device does not support Thunderbolt, Alt HDMI, or Displayport over USB-C, You will not be able to use the video ports.
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Reviewed in the United States on February 23, 2023
★★★★★ 5
Great product, had an issue, they took care of it.
Purchased this to allow me to run one cable to a laptop and have monitor/power/external accessories all connect easily. After a few days I noticed my monitor would disconnect and I would need to unplug and replug the device. Reached out to Anker support and they responded quickly to help diagnose and then sent a replacement unit very quickly. Was seriously impressed with the support and follow up.
Item has been working perfectly since - makes a great clean setup.
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Reviewed in the United States on September 8, 2023
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