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Description
Mouse CXCL12 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample handling and requirements: Plasma: Collect specimens using EDTA or heparin as an anticoagulant. Centrifuge at 1000g for 15 minutes at 2 8C within 30 minutes of collection. Remove the supernatant for analysis or store at 20C
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample handling and requirements: Plasma: Collect specimens using EDTA or heparin as an anticoagulant. Centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for analysis or store at -20°C or -80°C. Avoid repeated freezing and thawing. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C. Avoid repeated freezing and thawing. Pre-Assay Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the gradient working solution of the standard: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 8000 pg/mL). Then dilute to the following concentrations: 8000 pg/mL, 4000 pg/mL, 2000 pg/mL, 1000 pg/mL, 500 pg/mL, 250 pg/mL, 125 pg/mL, and 0 pg/mL. Serial Dilution Method: Add 500 μL of universal diluent to each of seven EP tubes. Pipette 500 μL of the 8000 pg/mL standard working solution into the first EP tube and mix thoroughly to make a 4000 pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a CXC motif chemokine 12 (CXCL12) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of CXC motif chemokine 12 (CXCL12) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | ||||||||||||||||||||||||||||||
| Source | Mouse | ||||||||||||||||||||||||||||||
| Synonym | Mouse C-X-C motif chemokine 12 ELISA Kit | ||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | ||||||||||||||||||||||||||||||
| Composition |
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| Background | CXC chemokine ligand 12 (CXCL12), also known as stromal cell-derived factor 1 (SDF1), is a chemokine protein encoded by the CXCL12 gene. It is ubiquitously expressed in many tissues and cell types. Stromal cell-derived factors 1-α and 1-β are small cytokines belonging to the chemokine family. Members of these chemokines activate white blood cells, typically in response to proinflammatory stimuli such as lipopolysaccharide, TNF, or IL-1. Chemokines are characterized by the presence of four conserved cysteines forming two disulfide bonds. They can be divided into two subfamilies. In the CC subfamily, the cysteine residues are adjacent to each other. In the CXC subfamily, they are separated by an intermediate amino acid. The SDF1 protein belongs to the latter group. Its signaling has been observed in several cancers. Its gene also contains one of 27 SNPs associated with an increased risk of coronary artery disease. | ||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | ||||||||||||||||||||||||||||||
| Test Range | 125-8000pg/mL | ||||||||||||||||||||||||||||||
| Applications | Plasma, cell culture supernatant |
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4.3 ★★★★★
Based on 12 reviews
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Product Reviews
★★★★★ 5
Bad auto driver install. Great mouse. Few of the left hand mfgs.
Style: Left-Handed, Pattern Name: Mouse, Color: Graphite
Really crappy auto driver install. Locked up my USB ports for my usb keyboard, usb mouse, all usb ports in fact. Had to reboot multiple times with an old style 5pin keyboard since the software screwed up my usb ports. All of them. Lucky I have a motherboard with an old style keyboard port. A reboot of W11 in safe mode, ripped out auto-installed software, registry keys and rebooted to regain control of my USB ports. I do not know what went wrong. Alls I know is Logitech loaded something, it locked up my USB ports and I had to rip everything Logitech out to get control again. I downloaded the software, installed it and it worked fine. I've had no problems since.
I'm an old lefty and as such am ambidextrous in most areas, since there just was not left handed oriented items readily available. You had to have left handed stuff made so you learned to do things right handed. I've developed carpal tunnel in both elbows and had purchased a couple of vertical mice, one left and one right. I took the left into work and was looking for a replacement. My other two were around $30 ea. I liked the right handed vertical mouse (Anker). They do not make a left handed version. The left handed mouse is ok, not as good as the Anker. I decided to go above my spend tolerance and get the Logi after reading many reviews. It has not disappointed me. The auto-install sucked. Never letting that happen again.
It is a nice smooth mouse. Fine movements that are easily adjustable. The hand position is excellent. I can use this all day without switching to the right because of elbow pain. If you have the pain, you know what I am talking about when it flares. It takes some getting used to the subtlety of the clicks when coming from lower cost mice. Soft and gently like a newborn's ....
One of my big criteria for a vertical mouse is a landing area for the outer two fingers (pinky and next to pinky for finger orientation challenged). When you go to a vertical mouse, it takes some adjustment to moving the mouse since you can't really press down with your palm and inside two fingers (the ones next to the outside two fingers). It is helpful to be able to use the two outer fingers and thumb to aid in moving the mouse. You have to experience a vertical mouse to understand. The right handed only Anker goes from an inch to a half inch in the length of my pinky below the buttons on the side. This is a really nice landing area for the outside fingers to assist moving the mouse around. You can squeeze a little and the mouse glides. The Logi mouse is a wee shorter than the Anker and the area below the buttons in a mostly straight 3/4". Both mice curve quickly down to the inside below this area. I was used to a vertical mouse by the time I purchased the Logi and I have no issues with the pinky dragging the table and the finger next to pinky resting on the landing. I move the mouse with no problems. It is the subtlety of how one interacts with these style of mice. The big plus, takes a long time before mouse use causes pain flareups. And this isn't just a pain and done. Depending on how one ignores the pain, often determines how many days life sucks. The Logi mouse minimizes the flare ups. I'm thinking of taking the Anker in and getting a right handed Logi for home. I like the Anker, I'm frugal and highly recommend it for getting your feet wet. I love the Logi (auto-install sucks) and will probably ignore my frugal tendencies and get a right handed one since I don't switch hands as frequently with the Logi in the mix. Don't have to suffer auto-install either.
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Reviewed in the United States on February 29, 2024
★★★★★ 4
Shape can be a game changer to reduce arm/wrist pain
Style: Right-Handed, Pattern Name: Mouse, Color: Graphite
Very easy to setup with PC, whether by bluetooth, usb, wifi. It does take some time to get used to the new design. The scrolling is very good and all functions are extremely responsive. So far, it is not causing any strain to my arm and wrist which is why I am trying this design as my older, normal shaped mouse, was causing me arm pain. They should include a quick easy to understand information sheet about the buttons. Need to download in order to customize 2 other buttons. All in all, so far I like it and definitely suggest it for people having arm or wrist pain from the angle and position of a regular shaped mouse.
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Reviewed in the United States on May 11, 2026
★★★★★ 5
Wonderful!!
Style: Right-Handed, Pattern Name: Mouse, Color: Graphite
Update -
After using this mouse for over a year I started experiencing something profoundly annoying. When dragging something from one place to another (often from monitor to monitor) I would accidentally hit one of the buttons by the thumb and it would close whatever I was working on. Especially during work where I do this often. I found there is an option to turn these buttons off in the software!!! Wonderful!!!
That was the absolute only thing I was having an issue with. Longevity has been perfect and I'll confess, I'm not particularly regular about cleaning it. I would buy this mouse again in a heartbeat.
I bought this at the same time (Feb '25) as the Logitech Ergo K860 Wireless Ergonomic Keyboard - Split Keyboard, for which I have also written a review.
I cannot say enough good things about both of these products. My neck, shoulders and back feel so much better than with my previous setup.
At the time of purchase, there were a number of reviews saying these failed fairly quickly. This has not been my experience and I am not very conscientious about cleaning it.
After almost a year of heavy use (8-10 hrs a day), the batteries are at 30% which I don't think I can complain about.
These products are not cheap, but for me they have been worth every penny and I cannot see myself going back. I'd buy them again in a heartbeat.
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Reviewed in the United States on January 28, 2026
★★★★★ 5
Easy setup, beautiful red!
Color: Red, Color: Red
I needed a mouse for an assignment when I asked my son for his. I loved it so much that I needed to buy myself one. I bought the red to match my computer (and to know that the navy blue is my son's) and it's a very sleek, not tooo vibrant color. It does come with both an USB & USBC controller (see pictures) and mine connected FAST to my computer. It does NOT come with a battery (most mice won't) so please grab a DOUBLE AA battery before it comes in so you will be ready. I have no issues with the mouse and loved the price point on this one. It's light weight and easy to setup. Then again it was a plug in/play setup, so if you're not tech savvy, you won't have issues with this one. Just choose a color and get it!
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Reviewed in the United States on April 8, 2026
★★★★★ 5
Cut the Cord and Never Looked Back!
Color: Grey
I'd been using a wired mouse forever and kept telling myself the cord wasn't that annoying. Spoiler: it was. The moment I switched to the WisFox 2.4G Wireless Mouse, I realized what I'd been putting up with all along — and there's no going back now.
Setup was dead simple. Plug the little USB receiver into the laptop, and it just works. No drivers to hunt down, no pairing headaches, no nonsense. The 2.4G connection is solid and responsive — I haven't noticed any lag or signal dropout, even when the receiver is tucked into a port on the back of the laptop. The cursor tracks smoothly across my desk surface without any skipping or stuttering.
The ergonomic shape is a genuine plus. It fits naturally in the hand during long sessions and doesn't leave my wrist feeling fatigued the way some flatter mice do. The buttons have a satisfying, tactile click without being loud enough to bother anyone nearby.
My one gripe — and it's pretty minor — is that it runs on batteries, which means you'll eventually need to swap them out. It's not a dealbreaker by any stretch, and the battery life has been reasonable so far, but if you're used to a wired mouse that just runs forever, it's a small adjustment to keep a spare AA or two around.
Pros:
Instant plug-and-play setup with the USB receiver
Reliable, lag-free 2.4G connection
Comfortable ergonomic shape for extended use
Great freedom of movement without cord clutter
Cons:
Runs on batteries — plan to keep a spare on hand
If you're still tethered to a wired mouse and on the fence about making the jump, this is a solid, no-fuss way to do it. Hope this helps!
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Reviewed in the United States on May 16, 2026