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Description
Human CXCL16 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Chemokine CXC-Motif Ligand 16 (CXCL16) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Chemokine CXC-Motif Ligand 16 (CXCL16) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Chemokine C-X-C-Motif Ligand 16 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | CXC chemokine ligand 16 (CXCL16) is a small cytokine belonging to the CXC chemokine family. CXCL16 is larger than other chemokines (254 amino acids) and consists of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain, and a cytoplasmic tail. It contains a potential tyrosine phosphorylation site, potentially allowing for SH2 binding. These unusual features of chemokines allow CXCL16 to be expressed as both a cell surface-bound molecule and a soluble chemokine. CXCL16 is produced by dendritic cells in the T cell compartments of lymphoid organs and by cells in the erythroplasm of the spleen. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.4 ★★★★★
Based on 8 reviews
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Product Reviews
★★★★★ 5
I LOVE MY Criss Cross Applesauce Office Desk Chair IS THE BEST
Color: Cream, Size: PU Leather(No Wheels)
The Criss Cross Applesauce Office Desk Chair is a unique and innovative addition to any workspace, offering a blend of comfort, functionality, and modern design.
What immediately stands out about this chair is its ergonomic design. The criss-cross frame provides excellent support to the back and promotes proper posture during long hours of sitting. This feature is particularly beneficial for reducing strain on the lower back and promoting overall spinal health, which is essential for those who spend extended periods at their desks.
The chair is upholstered with high-quality materials that offer both durability and comfort. The cushioned seat and backrest provide ample support while maintaining a plush feel, ensuring comfort throughout the workday.
Another notable feature is the adjustable height mechanism, which allows users to customize the chair to their preferred seating position. This versatility ensures that the chair can accommodate individuals of varying heights and preferences, enhancing overall ergonomic comfort.
In terms of aesthetics, the Criss Cross Applesauce chair boasts a modern and sleek design that complements contemporary office decor. Its minimalist appearance blends seamlessly into any workspace environment, adding a touch of style without compromising functionality.
Assembly of the chair is straightforward, with clear instructions provided for easy setup. The sturdy construction and stable base ensure reliability and safety, providing peace of mind for users.
While the chair excels in comfort and design, some users may find the criss-cross frame design to be unconventional compared to traditional office chairs. It's important to consider personal preferences and ergonomic needs when choosing a chair that best suits individual comfort requirements.
Overall, the Criss Cross Applesauce Office Desk Chair combines ergonomic support with modern aesthetics, making it a practical and stylish choice for enhancing comfort and productivity in the workplace. Its blend of functionality, comfort, and contemporary design earns it a recommendation for anyone looking to upgrade their office seating experience.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 10, 2024
★★★★★ 5
Perfect little black chair
I bought this chair for four reasons:
1. My favorite color is black, but the company offers different color options.
2. The height of the chair was exactly what I was looking for. I did not want a tall back for the chair. The chair is close to the ground, which is a plus for me, my back, and my knees as I get older.
3. The chair comes with the option of wheels or no wheels; I chose the no wheels option, as I get dizzy and sick when I spin around for too long.
4. The chair’s wide back and seat design are perfect for me, since I prefer to sit criss-cross in a chair.
Pluses: It reclines, is comfy, and can be cleaned and moved easily.
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Reviewed in the United States on January 7, 2026
★★★★★ 5
Great chair, highly recommend!
Color: Cream, Size: PU Leather(No Wheels), Color: Cream, Size: PU Leather(No Wheels)
Very nice looking chair! It is comfortable, classy, and durable unless you have a cat like mine who used it for his claws. It was easy to put together. All of the hardware was of good quality and everything fit perfectly. My 14 year old daughter even put it together herself. We actually bought two of these. The other one is in my daughter‘s room for her vanity. I highly recommend them whether you’re buying it to sit crisscross or just for roomy comfort. It is a very comfortable chair and looks elegant.
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Reviewed in the United States on March 15, 2026
★★★★★ 5
New Favorite Desk Chair
This chair is exactly what I was looking for! It is simple to put together, very sturdy and very comfortable. I typically tend to sit criss cross while at my desk and I can do that very comfortably with it. Im a bigger girlie so a chair that can hold weight or deal with me shifting positions was very important to me.
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Reviewed in the United States on May 25, 2026
★★★★★ 4
Comfortable chair
Color: Cream, Size: PU Leather(No Wheels)
Ordered this chair for my daughter for Christmas. It's a great chair. It's very comfortable. She wishes it had wheels. I didn't see a way to add wheels to this base, either. Still a good chair. You have to make sure where you put it you can spin to get out of it. Pushing it back and forth on carpet doesn't work well.
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Reviewed in the United States on March 8, 2026