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Description
Mouse SLC30A5 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment:
1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Solute Carrier Family 30, Member 5 (SLC30A5) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Solute Carrier Family 30, Member 5 (SLC30A5) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Solute Carrier Family 30, Member 5 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Solute carrier family 30, member 5 (SLC30A5), also known as ZnT-like transporter 1 (ZNTL1 or HZTL1) or ZNT5, is encoded by the SLC30A5 gene. This gene encodes a member of the SLC30A/ZnT family of zinc transporters. ZnT proteins mediate cellular zinc efflux and zinc sequestration into membrane-bound organelles. The encoded protein functions as a heterodimer with zinc transporter 6 in the early secretory pathway and also regulates zinc sequestration into secretory granules in pancreatic β-cells. Multiple splice variants of the transcript encoding this gene have been observed, and a pseudogene for this gene is located on the long arm of chromosome 19. It may transport zinc into β-cells for insulin crystal formation. It partially regulates cellular zinc homeostasis. It works with ZNT7 to activate zinc-requiring enzymes, alkaline phosphatases (ALPs). It delivers zinc to the lumen of the Golgi apparatus and to the vesicular compartment where ALPs reside, thereby converting apoALPs into holoALPs. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.2 ★★★★★
Based on 23 reviews
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Product Reviews
★★★★★ 4
Vintage 60's cool, but sizing is a difficult decision
I can't believe I haven't owned one of these for the last 40 years or so. To put one on after all these years takes one right back to the questionable days of one's youth when you wore these bare chested underneath while tooling around on your Kawasaki 500 triple. Well it will take you back if you haven't let yourself go too far!
I received both a large and a medium in black (love the free returns on clothing, I always order two sizes so I get the right fit). They are both made in Mexico and look very neat and well made, the denim is on the thin side but OK for warm weather. Nowhere is there any tag saying shrink to fit or shrinks two sizes. The tag says machine wash cold, tumble dry medium.
The large is quite large on me (I'm 5'9" 168 lbs trim and athletic build, more often than not I wear large in T shirts and jackets), the sleeves are way too long and loose, body too long and loose. Would have to shrink drastically to fit me, I really doubt it would shrink anywhere near that much. This type jacket is just all wrong if it's too loose fitting, witness the BLACK color one in the model photos, just not too cool.
The medium fits much much smaller but IMHO perfect for this style jacket, a classic fit like Steve McQueen or Robert Plant would have sported, high waist and tight shoulders with sleeves rolled back a little. If you look at the model in the 'HARRINGTON' color you have the idea. It will be totally bitchen on today's big black BMW oilhead. This is the youthful fit I was looking for but any shrinkage would be too much. I am conflicted because I would like to wash this jacket and break it in, put a little wear and tear on it. But I wouldn't dare dry it with heat. I might try washing it in cold water and then line dry or I may just dry clean it. I am tempted to go out and drag it up and down the gravel driveway and run it over with my truck a few times till it acquires some of the same attitude it inspires in me. All I need now is a Psychedelic soundtrack and I'm all set to relive the glory days, better than the first time around.
A little research on the Levi's website reveals they have a regular fit and a slim fit in addition to this relaxed fit which is the only one available on Amazon. I find that this relaxed version works well in the medium size anyway, it seems to be relaxed only in the body, not so much in shoulders or sleeves.
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Reviewed in the United States on January 3, 2015
★★★★★ 5
Fit True to Size
Special Size Type: Standard, Size: Medium, Color: (New) in the Sandbox
Good quality you'd expect from Levis. Fit on the very slightly snug size of true to size. Very nice jacket.
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Reviewed in the United States on May 31, 2026
★★★★★ 5
Large fits well, comfortable, though a bit more flex to it than expected
Special Size Type: Standard, Size: Large, Color: Colusa/Stretch
Very nice, though it is stretchier than expected. I purchased a large. The fit is good for me at 5'11" / 200lb, average build. My shoulders are a bit broader than most and the fit is maybe just a weeeeee bit tight, under the arm when reaching for something. The arm length and overall length are both great. This will be a mid-season jacket so I do not plan to wear a hoody or other bulky item under it. It would work, but I think a bit too snug for me. I reccomend washing it immeidately then taking a hot iron with steam to the pockets and collar which I feared were trying to start and curl up. They need to be trained early! I am more pleased than expected.
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Reviewed in the United States on March 30, 2025
★★★★★ 5
TRIED OTHER DENIM JACKETS LEVI THE ONLY WAY
This jacket is exactly what I was hoping for timeless, clean, and super versatile. The black denim looks sharp and goes with everything, from jeans to chinos. The fit is true to size and hits that perfect spot between fitted and comfortable. I can move around easily without it feeling stiff, and it breaks in nicely after a few wears.
The quality really stands out — solid stitching, durable buttons, and that signature Levi’s structure that just looks good no matter how you style it. It’s warm enough for cool days but still light enough to wear indoors or layer over a hoodie.
Im 180lbs 6’2 for reference, If you’re after that classic denim jacket look that never goes out of style, this one’s it. I get compliments every time I wear it. Also I’ve owned it a year now and it still looks great.
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Reviewed in the United States on November 2, 2025
★★★★★ 5
Levi Sun Surf Type 3 Denim Trucker Jacket - Med Wash
Special Size Type: Standard, Size: Medium, Color: (New) Sun Surf, Special Size Type: Standard, Size: Medium, Color: (New) Sun Surf
Levi Type 3 Trucker jackets are the best you can buy. Every one should own a couple of these. I have every color of the Premium versions, that I bought straight from Levi. I've been wanting to try this Sun Surf type 3 from Amazon for a while now. I bought this one to wear over my hoodies on the cooler days and nights, because of its wide body fit. It's not as slim as the Premiums, but it's just as official. It's super soft and lightweight. The fit and appearance is on point. It looks just like the pictures. Love it! I might get a dark wash as well. Amazon + Levi for the win again!
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Reviewed in the United States on May 15, 2026
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