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Description
Rat SOD ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a superoxide dismutase (SOD) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of superoxide dismutase (SOD) in the sample. The absorbance (OD value) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Superoxide Dismutases ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Superoxide dismutase (SOD) is an enzyme that catalyzes the conversion of superoxide into oxygen and hydrogen peroxide through a dismutation reaction. It is widely found in various animals, plants, and microorganisms and serves as an important antioxidant, protecting cells exposed to oxygen. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.1 ★★★★★
Based on 5 reviews
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Product Reviews
★★★★★ 5
Best deal and beautiful sound
I bought this one on sale to replace my old Pioneer VSX-1120-K 7.2 ch receiver which did not support 4K video and new Atmos sound profile. I would say, it was the best deal in this category since it offered 100 watts/ch , 9.2 ch, 8k/4k supported, and nearly 50% price cut when on sale! The sound quality is amazing, especially after using Dirac live to measure and auto adjust all speakers’ sound curves, making my home theater sound in a different level. The high is very clear but not harsh while the low is soft and nice punch. I also like the Onkyo App which helps to control the receiver in many ways. I can use it together with other online music streaming services app without touching the remote controller any more, very convenient! The only a small problem it has, is that it’s BD/DVD HDMI input sometimes does not work well with Amazon fire stick. It makes the firestick restart over again and again, so I have to use the other input such as game input to work smoothly with the firestick.
Update: I have had this receiver for more than a year now. I would say, I love it more and more! It not only supports 4K/8k video through HDMI, but also has so many listening sound models to choose from, and the App on smartphone is a very convenient tool. One of my favorite functions of it is the Dirac live function. I used to analyze the sound characteristics of each speaker, and fine tune the frequencies on them, making my home theater speakers up to a very high level, making every single sound frequency is in its best condition. This certainly compensates the shortcomings of certain sound frequencies in each speaker. Many times I just sat in my home theater listening to the music from Spotify without watching movie, and it was so joyful. I am changing the rate from 4 stars to five!
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Reviewed in the United States on March 26, 2024
★★★★★ 4
The basic set-up is relatively easy which was a big plus for me......
First let me start off by saying that I am not an audiophile. So, this review will not get into the weeds of the more complex settings this receiver is capable of. For example, the Dirac room measuring option. No way was I attempting to mess with that. Especially, after reading that some reviewers who actually have experience with Dirac found it could be confusing/difficult to set up. Besides I come from a previous Onkyo the TX-SR607 which had the AccuEQ Room Calibration similar to this receiver, so I used that instead with no issues. As far as hooking up the speakers it's pretty straight forward. If you have banana plugs, use them, seriously. I originally planned on using banana plugs but in order to save time I decided against that. But I wish I had taken the extra time to go with bananas because trying to thread the speaker wire into the speaker terminals is tedious, there not much room between the terminals and even me with my skinny fingers struggled a bit. Wish Onkyo designed their terminals side by side, but I think only Denon does that. Anyway, after all the speakers were connected (I'm currently using a 5.1.2 setup) and I connected my tv, Blu-ray player, CD player, etc., etc., I ran the AccuEQ Room Calibration, and I was good to go. I did change the some of the settings like speaker volume that AccuEQ has set but nothing major. I have noticed that I need to turn the volume up much higher on this Onkyo than on my previous one. What I mean by that is that on my previous Onkyo I would set the volume indicator at around 30-35 tops for movies. With this one it's more like 40-45, maybe how the volume is measured is different on this one. The sound is excellent for movies and music I have no complaints there. I have only tried a few of features so far like Airplay but will dig deeper into the manual (I downloaded from the internet) as time goes by. I will say as far as build quality is concerned it's not bad, but IMO my old Onkyo, a 2009 model, had the look and feel of a more expensive receiver. But it's what's inside that matters most so hopefully this one last as long as my previous Onkyo.
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Reviewed in the United States on February 22, 2024
★★★★★ 1
Sound unexpectantly blasted for no reason. It malfunctioned!
I spent hours between installation and set up! I had to return it. I turned on NET (network) to play Sirius Xm and the volume was low but after a few minutes the sound turned all the way up to maximum completely on its own? This happened at least two more times. I contacted ONKYO and was told it is clearly defective and that their is no solution to correct the problem, and that I should return it immediately! I purchased the newer model and am pleased with its performance! It’s unfortunate because the first one that had the issue with the sound really played nice, but having sound change its volume so suddenly to its full capacity was not what I expected, nor felt comfortable enough to keep as I said I was told to return it, which I did! My advice here would be to purchase the newer model, which seems to be much more stable in its performance, and the quality is excellent.
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Reviewed in the United States on November 29, 2025
★★★★★ 5
Absolutely Love This Receiver
The Onkyo TX‑NR7100 has completely transformed my home theater. The sound quality is rich, detailed, and powerful, and the 9.2‑channel setup gives movies and music a level of immersion I didn’t realize I was missing. Dirac Live right out of the box is a huge win — the room correction made an immediate, noticeable improvement.
Setup was smooth, the interface is clean, and everything from streaming to switching inputs feels fast and reliable. It also plays perfectly with the rest of my system, and the THX certification really shows in how cinematic everything sounds.
I absolutely love this receiver. It’s one of those upgrades that makes you wonder why you didn’t do it sooner.
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Reviewed in the United States on February 20, 2026
★★★★★ 5
Incredible 9.2 Surround receiver at an unbeatable price.
I may return at a future date to give a more complete review, but others that are much more knowledgeable about audio equipment than I have already done so.
For me, the Onkyo (Onkyo TX-NR7100 9.2-Channel 8K/4K Network A/V Receiver) replaced a much older (2001) TX DS787 5.1 100 W Surround receiver that listed new for around $1050. I probably didn't pay quite that much but certainly something near $900. It was great for its time, perhaps even advanced with THX, Dolby, and other listening modes.
But it didn't have: HDMI inputs or outputs, any BlueTooth capability, no hard wired or WiFi connectivity or basically any operating or connection modes that most all modern receivers have. This turned into a big problem with modern LED/LCD/OLED TVs, Alexa and other now common devices.
I bought my new Onkyo TX-NR7100 from Amazon for $625. Other retailers (e.g.Best Buy) advertise it for up to $1200, so Amazon's price is outstanding. Set up was far more complicated (for me) than any previous receiver that I ever owned, mostly because there were a very large number of back panel input and output jacks, to and from the TV, as well as speaker outputs for 9.2 surround.
Suffice it to say that once everything was connected properly (I made a few mistakes along the way), I was completely thrilled. The On Screen Display, completely accessible either from the front panel or the remote was far superior to anything I had ever seen before. Literally every operating parameter is accessible to the user. And I used most of them.
It is also completely WiFi ready so my 150 Gbit home Wifi network lets it connect wirelessly and stream music error free. BlueTooth is also another way to connect almost any device to it for audio and audio/video playback if you connect the digital connections to and from a modern TV. It also speaks and listens to Alexa, although I must confess that I haven't played around with that much yet.
This is already much longer than I had intended, so let it suffice to say that the Onkyo TX-NR7100 is an absolutely incredible receiver for an incredible price. I'd give it 8 stars if I could.
JM TEPPER
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Reviewed in the United States on November 7, 2024