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Description
mIHC Dye (TSA540)Description mIHC Dye (TSA540) is a high performance fluorescent dye designed for multiplex immunohistochemistry (mIHC) based on tyramide signal amplification (TSA) technology. It enables robust, covalent fluorescent labeling on formalin fixed paraffin embedded (FFPE) tissues, frozen sections, and tissue microarray (TMA) slides, supporting multi target, in situ biomarker detection in a single tissue section. This reagent provides strong, stable
Description
mIHC Dye (TSA540) is a high-performance fluorescent dye designed for multiplex immunohistochemistry (mIHC) based on tyramide signal amplification (TSA) technology. It enables robust, covalent fluorescent labeling on formalin-fixed paraffin-embedded (FFPE) tissues, frozen sections, and tissue microarray (TMA) slides, supporting multi-target, in situ biomarker detection in a single tissue section.
This reagent provides strong, stable fluorescence with Ex/Em: 520 nm / 540 nm, compatible with standard fluorescence microscopes and multispectral imaging systems. It delivers greatly amplified signals compared to conventional immunofluorescence, ideal for low-abundance antigens and spatial profiling of the tissue microenvironment.
Key Features
TSA-based signal amplification: Ensures high sensitivity and high signal-to-noise ratio, suitable for detecting low-abundance antigens.
Covalent binding: Binds to tyrosine residues covalently, maintaining excellent stability during antibody stripping and repeated staining cycles.
Broad Compatibility: Applicable to FFPE tissues, frozen sections, and TMA slides, meeting diverse experimental needs.
Spectral Characteristics: 540 nm spectral channel, matching AF514, Opal 540, and similar orange-yellow fluorophores.
Easy to Use: Ready-to-use formulation with included signal amplification reaction buffer, no complex pre-processing required.
Application Scope: For research use only; not for in vitro clinical diagnosis or human trials.
Principle
TSA540 adopts horseradish peroxidase (HRP)-conjugated secondary antibodies to activate fluorescently labeled tyramide substrates. The activated tyramide rapidly forms covalent bonds with tyrosine residues near the antigen-antibody complex, forming stable fluorescent deposits. After that, the antibodies can be stripped by heat repair without removing the dye signal, allowing sequential labeling of multiple targets on the same tissue section to achieve multiplex staining.
Storage & Stability
Storage Condition: Store protected from light at 2–8 °C; avoid direct exposure to strong light.
Shelf Life: Stable for 12 months from the date of receipt when stored according to the required conditions.
Composition
TSA Monochromatic Fluorescent Dye 540, Signal Amplification Reaction Buffer
Application
Mainly used for scientific research of immunohistochemical staining of tissues, paraffin sections, and TMA chips; not for in vitro clinical diagnosis or human trials.
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4.5 ★★★★★
Based on 5 reviews
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Product Reviews
★★★★★ 4
Toothbrush is toddler size only
Color: Pink
This toothbrush is more for toddlers and not for school age children. The water flosser portion can be for older children
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Reviewed in the United States on January 22, 2026
★★★★★ 5
Worth the money!
Color: Lightgreen
Absolutely amazing product!
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Reviewed in the United States on March 13, 2026
★★★★★ 5
Perfect beginner set
Color: Lightgreen
Love this!!! The toothbrush heads are a little flimsy but overall really liked this for my 3 year old.
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Reviewed in the United States on February 23, 2026
★★★★★ 5
Super Cute, Fun, & Effective
Color: Lightgreen
This set of water flosser and electric toothbrush are just adorable! The shapes of them fit in the hands really comfortably, but also add to the cuteness as the shapes of the animals!
Yes, this is meant for kids - so the toothbrush heads are small (for little kids), and the water flosser doesn't spray terribly hard. BUT they're still very effective.
I especially like the water flosser. It can be taken into the bathtub/shower while bathing, so the spray doesn't end up going places we don't want water to go. It has a small tank of water, and I recommend using room temp water (rather than cold) so that it's comfortable on the gums and teeth. Also to note, the flosser doesn't work when it's sideways or upside-down - it needs to be held upright.
This set is so cute, easy to use, easy to re-charge, and very comfortable. It's also very good quality - a great purchase. I love it!
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Reviewed in the United States on March 17, 2026
★★★★★ 4
4 year old approved!
Color: Lightgreen, Color: Lightgreen
Love that this is a complete set, down to the wall block for the charger!
My daughter is 4 and absolutely loves this set. I personally feel like the toothbrush head is a tad small, but it does do the job well. Its a soft brush head, with plenty of replacement heads. The toothbrush and the flosser both arrived fully charged, and so far we havent had to recharge it, but we're only a few days in. So, it seems to have a pretty good battery life so far. (I will update my review if anything changes on that aspect)
The toothbrush and flosser both stand upright independently on the counter, without tipping over.
The water flosser is hard fir my daughter to do by herself. She sprays water everywhere, as theres several buttons on it and she cant seem to remember in time which button turns it off. I help her with flossing and its been going fine.
Whether youre on high or low the water pressure is not too hard for a kids mouth. In fact, I think the low setting does nothing but rinse the mouth and basically teach the habit of flossing. We use the high setting and it does and OK job. I am a little old school though, and feel like nothing will top a good ole dental floss flossing, but water flossing is a safer route to avoid cutting gums or getting floss stuck between close teeth.
The sound level is minimal, a fraction of the sound level my adult water flosser makes. It doesnt shake/vibrate too badly in the hand and theyre bith comfortable to hold, and size appropriate for my (4yrs) daughters hands.
It comes in a really nice box, packaged perfectly, making this a great gift for your little ones. The colors and theme makes brushing teeth exciting and fun again for your littles :)
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Reviewed in the United States on October 25, 2025
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