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Description
Biotin Rat Anti-Mouse CD14 Antibody (S-R609)Product Specification Host Rat Antigen CD14 Synonyms Monocyte differentiation antigen CD14; Myeloid cell specific leucine rich glycoprotein; Cd14 Location Cell membrane Accession P10810 Clone Number S R609 Antibody Type Rat mAb Isotype IgG2a,k Application FCM Reactivity Ms Positive Sample RAW 264. 7 Purification Protein G Concentration 0. 2 mg ml Conjugation Biotin Physical Appearance Liquid Storage Buffer PBS pH7. 4, 0. 03% Proclin 300 Stability &
Product Specification
| Host | Rat |
| Antigen | CD14 |
| Synonyms | Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; Cd14 |
| Location | Cell membrane |
| Accession | P10810 |
| Clone Number | S-R609 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | RAW 264.7 |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
CD14 is a 375-amino-acon, pattern-recognition glycoprotein that circulates in plasma as a soluble 55 kDa fragment (sCD14) shed by GPI-anchor cleavage or exists as a 55 kDa, glycosylphosphatidylinositol-anchored membrane receptor (mCD14) on myeloid cells; in both forms it acts as the critical co-receptor for Toll-like receptor 4/MD-2, enabling innate immune detection of lipopolysaccharide and other bacterial lipoglycans, thereby triggering NF-κB–driven cytokine storms, and its serum concentration rises acutely in sepsis, making it both a biomarker and a therapeutic target for endotoxin-driven inflammation.
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