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Description
Human TRIM29 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Tripartite Motif-Containing Protein 29 (TRIM29). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Tripartite Motif-Containing Protein 29 (TRIM29) in the sample. Absorbance (OD) is measured at 450 nm using a microplate reader to calculate sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Tripartite Motif-Containing Protein 29ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Tri-domain-containing protein 29 (TRIM29) is a protein encoded by the TRIM29 gene. The protein encoded by this gene belongs to the TRIM protein family. It has multiple zinc finger motifs and a leucine zipper motif. It is thought to form homo- and heterodimers and participate in nucleic acid binding. Therefore, it may act as a transcriptional regulator, participating in carcinogenesis and/or differentiation. It may also play a role in suppressing radiosensitivity, as it is associated with the ataxia phenotype. It has been shown to interact with TRIM23 and GCC1. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.1 ★★★★★
Based on 16 reviews
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Product Reviews
★★★★★ 5
Classic Craftsmanship That Keeps Perfect Time
This is an outstanding watch that blends craftsmanship, reliability, and timeless design. From the moment you put it on, it feels solid and well-built, with a level of precision that reflects true attention to detail.
One important thing to understand is that this is a mechanical watch, not automatic, which means it requires manual winding. Personally, I wind the crown every morning—it’s become part of my daily routine and adds a certain charm and connection to the watch that you simply don’t get with battery-powered or automatic models.
Once fully wound, the power reserve lasts around 24 hours, and impressively, the watch keeps extremely accurate time throughout. I’ve found it to be consistently reliable, rarely needing any adjustment.
Rather than being an inconvenience, the manual winding becomes part of the experience—it’s a reminder of the craftsmanship behind the piece. If you appreciate traditional watchmaking and don’t mind a small daily ritual, this watch is truly exceptional.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 27, 2026
★★★★★ 5
Pr 516 tissot
Muy buen reloj creo q lo mejor q tiene es q es cuerda manual esa conexión con el reloj es brutal buenos materiales y acabados
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Reviewed in the United States on August 17, 2024
★★★★★ 1
Too big and ugly
Nothing worth the price
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Reviewed in the United States on August 5, 2024
★★★★★ 5
Watch that's not a Speedy with its own 1970s racing heritage from the same group that owns Omega
Beautiful watch with its own heritage, yes it's at the upper end for a Tissot, but the high caliber movement used other brands like Omega and Longines as well as the superior fit and finish, justify the price of this watch.
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Reviewed in the United States on January 28, 2026
★★★★★ 5
What a Watch !!!
It's truly a very good watch, and I’m quite happy with it. Let me break down the reasons why:
The design: the front, the dial, the bezel, the case
It has an outstanding design and looks incredible on your wrist. It gives you that feeling of wanting to wear the all day, just because of how good it looks. The case dimensions are really nice—at 41mm, it’s relatively large but still within the wearable limit. The thickness of the case also helps a lot, since it doesn’t have an automatic movement or a date function, it’s much thinner than other Tissot chronographs and doesn’t feel like you’re carrying a rhinoceros on your wrist. The proportions and the positioning of the subdials give it a very premium feel. Of course, it’s not a Rolex Daytona, but that’s not what you're looking for here. What you’re looking for is something original, different, with a distinct racing look and bold colors, without crossing into gaudy territory.
The movement
I think having a manual movement in this watch is a total win. If you’re considering this watch, chances are you already own others with date functions and automatic movements—and if you don’t, it would pair nicely with this one. This isn’t meant to be a daily watch, for me. It’s a watch for relaxing, going out for drinks with friends, spending time with family, it’s more laid-back, more of a weekend watch. And for those moments, you don’t need to know the date, you just need to enjoy the experience. For daily wear, there are plenty of other options in all price ranges, from a Seiko 5 to a Datejust. The manual movement on this watch gives you a sense of connection to it, something special. It really adds a lot to the experience.
The price
Sure, it’s not a Seiko 5, but for what it offers—a chronograph, manual movement, from a brand with history and pedigree, without being a cheap knockoff of the Daytona—it’s an absolute win. This is one of those watches that adds to any collection, whether you’re just starting out or already have a box full of watches worth thousands of dollars. It won’t feel out of place, and you’ll always find an occasion to wear it.
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Reviewed in the United States on December 29, 2024
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