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Description
Mouse IL-12 P70 Culture Media OneStep ELISA KitProduct Specification Antigen IL 12 p70 Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Ms Purification Protein A Stability & Storage 12 months from date of receipt reconstitution, 2 to 8'C as supplied. Kit Precision Intra assay: 2. 6% Inter assay: 6. 9% Sample type Cell culture supernatant Assay type Sandwich (quantitative) Sensitivity 5. 205 pg mL Range 7. 81 pg mL 500 pg mL Recovery Cell culture supernatant: 102% Assay time
Product Specification
| Antigen | IL-12 p70 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Ms |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 2.6%； Inter-assay:6.9% |
| Sample type | Cell culture supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 5.205 pg/mL |
| Range | 7.81 pg/mL – 500 pg/mL |
| Recovery | Cell culture supernatant: 102% |
| Assay time | 60 minutes |
| Species reactivity | Ms |
Background
Mouse IL-12 is a critical pro-inflammatory cytokine that bridges innate and adaptive immunity. It is a heterodimeric protein composed of p35 (IL-12A) and p40 (IL-12B) subunits, forming the bioactive IL-12(p70). Primarily produced by antigen-presenting cells like dendritic cells and macrophages upon pathogenic stimulation, its pivotal role is to drive the differentiation of naïve CD4⁺ T cells into T helper 1 (Th1) cells and to potently induce interferon-gamma (IFN-γ) production from both T cells and natural killer (NK) cells. This establishes a robust cellular immune response essential for defense against intracellular pathogens such as viruses and bacteria. In biomedical research, Mouse IL-12 is a cornerstone for studying Th1-mediated immunity, evaluating vaccine efficacy, investigating antitumor immunotherapy strategies, and modeling autoimmune diseases like experimental autoimmune encephalomyelitis (EAE).
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