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Description
mIHC Dye (TSA520)Product Specification References 1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008. 9. 2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79 80, 105. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46 58. 4.
Product Specification
| References | 1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. 2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. Stack, E.C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. 4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. |
| Theory | Multiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels. |
| Description | There are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases! Principle of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating. |
| Composition | TSA monochromatic fluorescent dye 520, signal amplification reaction solution |
| General Notes | 1. Formalin-fixed wax blocks or glass slides, large tissues or TMA, and sealing wax cannot be obviously damaged. 2. For glass slide samples, the tissue needs to be close to the glass slide to avoid wrinkles, and the glass slide must not be damaged, scratched or stained. 3. The tissue should contain at least more than 1000 cells. 4. The wax block needs to be embedded in solid tumor tissue, and necrotic tumor tissue, fine needle punctures, and cell slice samples will affect the staining effect. 5. The tissue should be fixed with 10% neutral formalin, and the normal fixation time is 18-24h. 6. The thickness of the slice is about 4um, and anti-detachment slides are used. It is recommended that the glass slides be prepared within one week after fixation. 7. Do not add any adhesive in the water bath slide fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove water, tap gently to remove water droplets, and do not wipe the slide with paper. 8. Place the glass slide on a hot plate at 45 °C for 30min (the natural air-drying time of the glass slide is not less than 1h). |
| Storage Temp. | Fluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods. |
| Applications | It is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments. |
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4.8 ★★★★★
Based on 14 reviews
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Product Reviews
★★★★★ 5
I would give it a million stars if could
Format: Paperback
I’m 11 and I love this book it’s so good and it’s good for adults too. Incase you didn’t know it has four books and the fourth one is so great. I definitely recommend for all ages 9 to 29 thank you for reading my review.
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Reviewed in the United States on February 27, 2025
★★★★★ 5
Growing pains
Format: Paperback
Middle school sucks. The transition from elementary to middle school is painfully isolating for Dani. In elementary school, Dani knew exactly where she fit in with her friends, she loved recess, sang songs they learned in school. Now, she doesn’t even have a friend in her lunch wave, there’s a bully, she arrives late to class and she has an annoying family. Everything is just wrong. Growing up is lonely, unless she can create a new bff with her magical notebook. Of course, there are consequences to her come-to-life drawings. This story is sweet, funny and well-illustrated. There are many little jokes inside each page too. This book is fun to read. It also uses a wish I always had as a child, to be able to create my own reality. I loved Making Friends, its references to Sailor Moon and its expressive characters. Truly a fun read.
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Reviewed in the United States on August 5, 2018
★★★★★ 5
Making Friends
Format: Kindle
This whole series is absolutely fantastic. I love the idea of a magical sketchbook that Dany can draw anything in. My favorite part (and my sister’s), is when Prince Neptune acts like he’s about to leave, and he kisses Dany. Then, he tries to yank the sketchbook out of her hands and he yells “So I will reshape this world to fit my needs!” with the most evil look on his face. It’s super funny. All of the backstories were well-developed, and the Madison and Amanda storyline really impressed me! I think anyone with a good sense of humor would love this book.
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Reviewed in the United States on November 25, 2021
★★★★★ 3
Cute story about friendship with a fantastical twist.
Format: Kindle, Format: Kindle
Amazon has been recommending me this book for quite some time now, and since I read a lot of reviews saying that the premise was very interesting/ unusual, I decided to give it a shot. I bought the Kindle version.
#1 The artwork: I'm a huge fan of graphic novels, so the art on the cover caught my eye immediately. I liked the overall style, and color palette. However I feel like the interior art falls flat sometimes. No doubt that the artist knows her stuff, but I felt like sometimes she might not have had such a strong handle on the characters. The line thickness tended to vary from panel to panel on some pages, and some characters didn't always look like they belonged in the same universe.
#2: The story (spoilers): The concept for this was very interesting, and I enjoyed it for the most part, but there were some things about the story I thought were unnecessary. I don't think Prince Neptune needed to be a character at all. To me, his only real purpose was to make Danny into a less likable version of herself, and to end the book when he destroyed the school. The ending I also thought was a little weird. I had read reviews about it beforehand, but It still caught me completely off guard. I know the story is fantasy, obviously, but I think it could've been toned down just a smidge. I think if the plot focused more on the relationship between Danny, Madison, and even Danny's other friends, I would've given it five stars. I found myself asking a lot of questions about them in terms of who they were to Danny, and why it seemed like they all stopped hanging out for seemingly no reason. A point was made that Danny had a different schedule than them, but I didn't even see her make an effort to preserve her relationships despite this.
One things I really want to praise the book on however, is the humor. There was a lot of subtle jokes for older readers that I chuckled at, that would probably fly over a kid's head.
For those who are wondering, there's no swearing/ inappropriate words, but some bullying/ teasing/ crying is depicted. There is also fantasy "violence", but it's nothing worse than what you'd see in a Saturday morning cartoon on Disney channel.
#3 Final Thoughts: Overall, I would recommend this book if you have young kids interested in this kind of thing. I'm not the target demographic for this, so I'm a bit more critical, however if someone had put this in the hands of 9 year old me, I would've absolutely loved it to bits.
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Reviewed in the United States on September 10, 2020
★★★★★ 5
Definitely a win for William!
Format: Hardcover
My eight-year-old grandson read this book in a matter of days. He said it was his favorite book of all times and that now he has a favorite new author!
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Reviewed in the United States on January 18, 2026