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Description
Mouse F11 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. 3. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Coagulation Factor XI (F11) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Coagulation Factor XI (F11) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Coagulation Factor XI ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Factor XI (FXI) is one of the enzymes in the coagulation cascade. Like many other coagulation factors, it is a serine protease. It is encoded by the F11 gene. FXI is produced in the liver and circulates as an inactive homodimer. The zymogen is activated to factor XIa by factor XIIa (FXIIa), thrombin, and FXIa itself. Because of its activation by FXIIa, FXI is a member of the "contact pathway" (which includes HMWK, probasic protein, factors XII, XI, and IX). Factor XIa activates factor IX by selectively cleaving the arg-ala and arg-val peptide bonds. In turn, factor IXa forms a complex with factor VIIIa (FIXa-FVIIIa) and activates factor X. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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4.4 ★★★★★
Based on 28 reviews
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Product Reviews
★★★★★ 5
Better than expected
This thing is great. I wasn't sure what to expect for the price, but it exceeded my expectations in every way. I have no complaints, quality is fine, the grind is fine, it's easy to use. I've used mine every day since I purchased it and it works good as new. This is my first manual burr grinder, so there could very well be better ones out there, but I am very happy with this purchase.
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Reviewed in the United States on May 27, 2026
★★★★★ 4
A good little grinder for small batches.
It's a good quality grinder, I use mine for my moka pot since the grind needs to be specific. It's very easy to use and its small enough to store away. The only issue is it takes some time and effort to grind for more than one person, so keep that in mind if you're brewing anything more than a cup of espresso or a moka pot. I've dropped mine more than I can count so store on its side (although its very sturdy and was not broken) A solid product.
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Reviewed in the United States on May 11, 2026
★★★★★ 5
Good quality and price
I purchased one in January and it works great, I like to have my coffee beans ground fine and It gives it fine. Just orered 2nd one for my friend.
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Reviewed in the United States on May 30, 2026
★★★★★ 5
Works very well as a pill grinder!
One of the clients I work with needs his pills crushed and dissolved. We have relied on the spoons method for years but some of his newer medications don’t crush well enough using that method. We tried the basic plastic pill crushers but they tend to get gummed up and don’t actually pulverize the pills. This fit our needs perfectly and the pills get crushed into a fine powder with ease. It is very easy to use and clean and takes very little effort to operate (at least for pills).
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Reviewed in the United States on April 17, 2026
★★★★★ 5
Great Little Grinder — Solid Build & Easy to Use!
I’ve really enjoyed using this manual coffee grinder! For the price, it’s an excellent find. The adjustable settings work surprisingly well — I’ve been able to get a consistent grind whether I’m brewing for espresso or French press, which isn’t always the case with more affordable manual grinders. The ceramic burr gives a nice, even grind without overheating the beans, which helps preserve the flavor.
It’s easy to use and straightforward to adjust. The grind size clicks into place firmly, so you don’t feel like it’s going to slip or change while you’re using it. One fill gives me enough coffee for about two cups, which is perfect for my morning routine or when I’m brewing a small batch.
The build quality feels sturdy and durable, with a sleek look that doesn’t feel cheap. The handle turns smoothly and doesn’t wobble, which makes the whole experience feel solid and satisfying. I’ve taken it on a short weekend trip already, and it’s lightweight and compact enough for travel or camping.
If you're someone who loves the ritual of grinding your own beans or just wants a reliable backup grinder for when the power’s out, I’d highly recommend this one. Great value for a tool that feels like it’ll last!
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Reviewed in the United States on June 10, 2025
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