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Description
Pacific Blue Mouse Anti-Rat CD11b/c Antibody (S-R630)Product Specification Host Mouse Antigen CD11b c Synonyms Itgam Location Membrane Accession Q9JI30 Clone Number S R630 Antibody Type Mouse mAb Isotype IgG2a,k Application FCM Reactivity Rt Positive Sample Lewis Rat splenocytes Purification Protein A Concentration 0. 02mg ml Conjugation Pacific Blue Physical Appearance Liquid Storage Buffer PBS, 25% Glycerol, 1% BSA, 0. 3% Proclin 300 Stability & Storage 12 months from date of receipt reconstitution, 2
Product Specification
| Host | Mouse |
| Antigen | CD11b/c |
| Synonyms | Itgam |
| Location | Membrane |
| Accession | Q9JI30 |
| Clone Number | S-R630 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Rt |
| Positive Sample | Lewis Rat splenocytes |
| Purification | Protein A |
| Concentration | 0.02mg/ml |
| Conjugation | Pacific Blue |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Rt |
Background
CD11b and CD11c are integral components of the integrin family, which are heterodimeric transmembrane glycoproteins involved in cell adhesion and immune responses. CD11b, also known as integrin alpha M (ITGAM) or Mac-1, pairs with CD18 to form complement receptor 3 (CR3), playing a crucial role in mediating the uptake of complement-coated particles and pathogens. It is expressed on various immune cells, including macrophages, monocytes, granulocytes, and NK cells. Similarly, CD11c, also called integrin alpha X (ITGAX), forms complement receptor 4 (CR4) with CD18 and is involved in similar adhesive interactions and phagocytosis. Both CD11b and CD11c are commonly used as markers for immune cells, with CD11b/c antibodies often employed in research to identify microglia in the brain and other immune cells.
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