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Description
Pacific Blue Mouse Anti-Human CD45RO Antibody (S-R401)Product Specification Host Mouse Antigen CD45RO Synonyms Receptor type tyrosine protein phosphatase C; Leukocyte common antigen (L CA); T200; PTPRC Location Cell membrane Accession P08575 Clone Number S R401 Antibody Type Mouse mAb Isotype IgG2a,k Application FCM Reactivity Hu Positive Sample Human PBMC Purification Protein A Concentration 0. 2 mg ml Conjugation Pacific Blue Physical Appearance Liquid Storage Buffer PBS, 1% BSA, 0. 3% Proclin 300
Product Specification
| Host | Mouse |
| Antigen | CD45RO |
| Synonyms | Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen (L-CA); T200; PTPRC |
| Location | Cell membrane |
| Accession | P08575 |
| Clone Number | S-R401 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Pacific Blue |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD45RO is an important cell surface marker in immunology that belongs to the CD45 family (also known as Leukocyte Common Antigen, LCA). CD45 is a highly polymorphic transmembrane protein primarily expressed on the surface of white blood cells, including T cells, B cells, natural killer (NK) cells, monocytes, and granulocytes, and plays a crucial role in lymphocyte development, activation, and signaling. Specifically, CD45RO is an isoform of CD45 that is highly expressed on memory T cells but expressed at lower levels or not expressed on naive T cells. Memory T cells are T cells that have been activated and survived during previous immune responses, enabling them to rapidly and strongly activate upon re-encountering the same antigen, thus accelerating and enhancing immune responses. Therefore, CD45RO is often used to distinguish and identify memory T cells. It serves as an important marker in immune phenotyping analysis, disease diagnosis, immune response monitoring, and immunotherapy research.
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