SKU: 50608387928

Human GM-CSF ELISA Kit

Sale price$202.50 Regular price$225.00
Save 10%

Pay in installments of $56.25 with ShopPay, AfterPay and Klarna

Shipping Estimate
USA
  • USA
  • CAN

Ships within 48 hours · Estimated delivery Aug 26 - Aug 31

Promo Codes Available:

For Your Every Summer RSVP, with Code: SUMMER15

Description

Human GM-CSF ELISA KitProduct Specification Description Detection Principle: The double antibody sandwich ELISA method was used in this experiment. The anti human GM CSF monoclonal antibody is coated on the microplate, the GM CSF in the sample and standard will bind with the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti human GM CSF polyclonal antibody was added, and the unbound antibody was washed away; Add

Product Specification

Description

Detection Principle:

The double antibody sandwich ELISA method was used in this experiment. The anti human GM-CSF monoclonal antibody is coated on the microplate, the GM-CSF in the sample and standard will bind with the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti human GM-CSF polyclonal antibody was added, and the unbound antibody was washed away; Add substrate solution (chromogenic agent), and the color of the solution is directly proportional to the bound target protein; Add termination solution; The absorbance was measured with a microplate reader.

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Detection Sample Type: cell supernatant, serum, Plasma

Loading Amount: 100ul

Kit Components:

Pre coated 96 well plate, standard, anti human GM-CSF detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, final stop solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.089-0.740  Pg/ml

Detection Range: 15.6 - 1000 pg/ml

Recovery Range: 85-104%

Storage Method: 2-8 ℃

Standard Curve

Background:

Granulocyte macrophage colony stimulating factor (GM-CSF), also known as csf-2, is a common β chain (β c)The polytrophic 30 kDa member of the cytokine family also includes IL-3 and IL-5. GM-CSF adopts &alpha with two intrachain disulfide bonds-Helical configuration. It is secreted by a variety of activated immune, mesenchymal, and epithelial cell types and circulates in the form of variable glycosylated monomers. During inflammation, it is upregulated in a variety of cell types, including encephalitic T cells, allergen exposed lung endothelial cells, and IgE activated mast cells. Mature human GM-CSF has 54% and 63% amino acid sequence identity with mouse and rat GM-CSF, respectively. The high affinity receptor of GM-CSF is bound by a 50 kDa ligand α Subunit (gm-csfrα)And 120 kDa signal transduction β C composition. According to reports, the stoichiometry of functional GM-CSF receptor is GM-CSF, gm-csfrα And β The 2:2:2 complex of C. It is worth noting that β The C subunit is shared by the receptor complex of IL-3 and IL-5, and IL-5 may pass through gm-csfrα And β C signaling. GM-CSF can also utilize syndecan-2 as a co receptor (14). GM-CSF has many functions. It induces the production of monocytes, neutrophils, and eosinophils from CD34 + stem cell precursors. It can synergize with IL-4 or Flt-3 ligands to induce the development and maintenance of bone marrow and dermal dendritic cells. It also functions as a chemoattractant for neutrophils and dendritic cells. GM-CSF promotes Th1 and Th17 cell-mediated autoimmune inflammation and inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils. In addition, it synergizes with G-CSF to promote the proliferation and invasion of tumor cells. Human GM-CSF immunoassay is a 3.5-4.5-hour solid-phase ELISA designed to measure the levels of human GM-CSF in cell culture supernatants, serum, and plasma. It contains recombinant human GM-CSF expressed in E. coli and antibodies against recombinant factors. Recombinant human GM-CSF has been shown to be accurately quantified. The results obtained using natural human GM-CSF showed that the linear curve was parallel to the standard curve obtained using the kit standard. These results indicate that the kit can be used to determine the relative mass value of human GM-CSF.

Shipping Notes
  • Free Standard Shipping on $100+ Orders to the USA.
  • Except Preorder products are shipped in 48 hours.
  • Delivery to the USA:
  1. Standard Shipping : 3-10 business days
  • If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy
SKU: 50608387928

Discover Niche Categories That Outsell

Top-Converting Item to Boost Your Average Order

4.6 ★★★★★
Based on 27 reviews
Sort
Highest Rating
Newest First
Oldest First
Product Reviews
B
Brittany Bingold
Pawtucket, US
★★★★★ 4
Breathable and light
Color: White, Size: Small, Color: White, Size: Small
This shirt is truly better in person than in the stock photos. Its just a bit more of a textured athletic material in person that looks nicer on than a simple loose standard t shirt. The material does still sit semi loosely and only mildly hugs curves but its still figure complimenting and looks just a hair above only casual. Its very very breathable and lightweight, excellent for the gym or summer running etc but also serves as a phenomenal base layer for hiking and sports that carry into colder climates and seasons . Its well made and fots my size well so the size chart feels spot on. This shirt is really only losing points for me in the cost department because while its decent its nothing special. Its a good basic but its not something I couldnt find almost everywhere else.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 23, 2026
J
Jewelry Trends
Port Orchard, US
★★★★★ 5
Soft, slinky cooling tee without that synthetic feel
Color: Grey, Size: Medium, Color: Grey, Size: Medium
I ordered this bamboo viscose CoolMax t-shirt in gray, size medium because I wanted something lightweight and breathable for workouts and just being around the house when it is hot. The standout feature to me is the super soft and slinky feel it has without the overly cool wet feeling you get from other shirts made from synthetic “cooling fabric.” It feels smooth and comfortable against the skin while still doing a great job keeping me dry and not clingy. The fabric is lightweight with a nice stretch, and it moves easily without losing its shape. The fit is very flattering with a clean shoulder line and a curved hem that sits right at the hips, so it looks polished enough to wear out, not just for the gym. The seams are flat and smooth, and the tagless design makes it easy to wear all day without irritation. It also holds up well after washing with no shrinking or shape issues. For sizing reference, I am 5'6", 119 pounds, 34B, with a 28.5" waist, and the medium fits me exactly like the model in the main listing picture, exactly. That should really help with sizing decisions if your build is similar. Highly Recommended!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 21, 2026
S
Sharon S
Lowell, US
★★★★★ 5
Lightweight and comfortable to wear.
Color: White, Size: Medium, Color: White, Size: Medium
I wanted something comfy, lightweight, breathable, durable, and cool to wear while working out. My old t-shirts leave a lot to be desired. This Bamboo Viscose Coolmax T-shirt meets all the above. The only downside for me is that I chose white, and the material is very thin and see-through. If you prefer to dress more modestly as I do, you’ll want to select a darker color. I planned to only wear it in the house or while sitting outside on the patio, so it’s okay. Comfort and breathability are important to me when the summer heat and humidity hit. The length and cut fit my physique and are comfortable to wear and move in. I got a medium, and it fits perfectly, which was really a nice surprise. Many tops tend to be too long or too tight in the hips. I’m 5’4” and 130 lbs. The top washes up nicely, but ended up with a few wrinkles. No big deal for me, though. Is it worth the price? I’d say it is based on the quality, fit, comfort, and breathability of the fabric. I’ve passed up buying similar tops when shopping because they were more expensive than this one. It’s a top to wear when working out, not for a night out on the town. It shouldn’t cost a fortune. At the time of this review, it’s on sale for $5.00 less, making it an even better bargain.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 3, 2026
S
Verified Purchase
southern belle
New York, US
★★★★★ 5
Better than any other!
Color: Set8-3, Size: Small
I've purchased this item twice now, they fit so we'll and are made great. The best part is the don't ride up or move around while I work. I'm very busy and move a lot, bending, squatting, twisting and these panties move with me. They don't shrink or fade. I highly suggest this item if your looking for a good set of panties that stay put without breaking the bank. Very comfortable and breathable. ****An update to my last review**** After washing the second pack that I got, they started coming apart. The legs seams were unhemmed and frayed in the washer.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 11, 2021
M
Verified Purchase
Miah Smith
Boise, US
★★★★★ 5
Stretchy and comfortable!
These underwear are exactly what I was hoping for! Love the look of having a seam without actually having one. They are soft, comfortable, and stretchy! Which is great for me because I have endometriosis so often have bloat days. Highly recommend! True to size.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 10, 2024

recommand products