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Description
Human UFD1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as an anticoagulant and centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Pre-Test Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with the capture antibody from the Ubiquitin Recognition Factor In ER Associated Degradation 1 (UFD1) ELISA Kit. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the concentration of Ubiquitin Recognition Factor In ER Associated Degradation 1 (UFD1) ELISA Kit in the sample. Absorbance (OD) is measured at 450 nm using a microplate reader to calculate sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Ubiquitin Recognition Factor In ER Associated Degradation 1 （UFD1）ELISA kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Ubiquitin fusion protein degradation 1 is a protein that is encoded by the UFD1L gene. The protein encoded by this gene forms a complex with two other proteins, NPL4 and VCP, and is required for the degradation of ubiquitinated proteins. Among other things, this complex controls the disassembly of the mitotic spindle and the formation of the nuclear envelope that closes after mitosis. Mutations in this gene are associated with Catch 22 syndrome as well as cardiac and craniofacial defects. Alternative splicing results in multiple transcript variants encoding different isoforms. UFD1L has been shown to interact with NPLOC4 | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, cell culture supernatant |
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4.4 ★★★★★
Based on 26 reviews
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Product Reviews
★★★★★ 5
All in One; Power Strip & Surge Protector
The Tripp-Lite Protect It Surge Suppressor is definitely the All in One tool for the job.
This power strip surge protector features seven handy outlets; number seven (at the end) is a wider plug surrounding that works wonders for plugging in that bulky AC adaptors that tend to take up so much space otherwise.
Its twelve foot cord allows easy extending that is almost as large as most rooms.
The red on/off tap button glows a pretty reddish/orange when lit, but does tap on and off rather easily, so it's best to either mount it on the wall or low on the wall/floor molding, or have it somewhere where the button will not be easily tapped, otherwise everything goes off.
The little Green LED light at the top of the switch ensures you that everything is in working order.
We had a pretty bad electrical storm not long after ordering this product and when all of the other lights in the house dimmed, the Television set didn't flicker, so at $21 dollars, I feel that it has already paid for itself.
One last note, for all those who are concerned with having only, or prefer, "Green" products, this power strip is ROHS Compliant; meaning it meets all requirements under the California Compliance Code.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 20, 2012
★★★★★ 5
BEST Surge Protector
Style: 12ft Cord (Black), Pattern Name: Single
Bought this based on so many rave reviews. I agree -- really great product. Nice long, thick cord and has 7 outlets, so I can plug in a lamp and all the "doodads" from my power recliner (power pack, remote, etc.) with ease. Slimline design makes it look nice, not bulky. Will DEFINITELY buy Tripp surge protectors from now on --- worth the money --- a great product !!!
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Reviewed in the United States on June 14, 2022
★★★★★ 5
Only get Automatic Shutoff surge protectors!
Style: 12ft Cord (Black), Pattern Name: Single
Surge protectors should STOP working once the surge protection is used up. These are among the few that operate like this. Always look for this feature, as surge protectors often wind up sitting under a desk for years. When it stops functioning as a surge protector, why would you want it to continue functioning as a passthrough power strip? There's no more protection. This line of Tripp Lite surge protectors function properly, and STOP working when it can no longer function as a surge protector.
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Reviewed in the United States on April 11, 2024
★★★★★ 5
Very nice! Perfect description and I can see these lasting a good long time!
Style: 12ft Cord (Black), Pattern Name: Single
We recently moved and needed much longer cords for some exercise equipment that requires surge protectors. We looked around and found these available in a variety of lengths. We bought two after measuring to make sure we got the right length, and we are very happy. They are the full length as advertised. Flexible cord with durable plugs and seem very sturdy! Nice, heavy wire covering - nothing flimsy about them! Very pleased with the quality for the price!
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Reviewed in the United States on February 26, 2020
★★★★★ 4
not sure if it’s worth the premium price tag
Style: 12ft Cord (Black), Pattern Name: Single
functions well. just not sure if it’s worth the premium price tag. there are other power strips that work just as well for cheaper. also is much bigger than I was expecting.
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Reviewed in the United States on June 4, 2025
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