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Description
Rat GPX1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a glutathione peroxidase 1 (GPX1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of glutathione peroxidase 1 (GPX1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Glutathione Peroxidase 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Glutathione peroxidase 1, also known as GPX1, is an enzyme encoded by the GPX1 gene. This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase detoxifies hydrogen peroxide and is one of the most important antioxidant enzymes. It is characterized by a polymorphism in the N-terminal alanine sequence, including three alleles with five, six, or seven alanine (Ala) repeats. The allele with five Ala repeats is significantly associated with breast cancer risk. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.7 ★★★★★
Based on 10 reviews
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Product Reviews
★★★★★ 5
Decent wipers for a great price
Size: 28+28+11
These fit my daughters Ford Escape perfectly and the day after installation, it rained. The wipers work really well and the windshield is now streak free once again and the price was right. Easy to change and look good.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 6, 2026
★★★★★ 4
easy install, ok - good performance
Size: 26"+16"+12"
2 minutes to install. Performs OK to good. I have had better wipers in the past, but there isn't any streaking and overall pretty good.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 2, 2026
★★★★★ 5
very good price
Size: 24"+19"+10A"
I needed to replace the wiper blades on my Jeep Wrangler JK, so I first went to a local auto parts store. The prices were pretty expensive, and they didn’t even have the correct replacement for my rear wiper blade.
That’s when I decided to order the UTOBOO 15"+15" Windshield Wipers with 13 Inch Rear Wiper Blade Replacement online, and I’m really glad I did.
The set arrived quickly and included everything I needed for both the front and rear wipers. Installation was very easy and only took a few minutes. They fit well on my Jeep, and the blades feel sturdy and made from good-quality materials.
After using them, they wipe smoothly and leave the windshield clean without streaks, which is exactly what you want, especially in rain or dusty conditions.
Overall, this is a great value compared to buying individual blades in-store, and it’s especially convenient since it includes the rear wiper replacement too. I would definitely recommend these to other Jeep Wrangler owners looking for an affordable and reliable option.
------Tip, don't forget to take off the green plastic, If you check the comments many people argues that the wipers don't work but in the pictures you can see they did not take off the green plastic protector. ----
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Reviewed in the United States on March 23, 2026
★★★★★ 5
Easy to install and works great!
Size: 26"+16"+12"
This was probably the easiest set of windshield wipers I've ever installed. It came out of the box with the correct locking hardware pre-installed for my car which made installation quick and easy. They felt solid in the hand, as if they're going to hold up over time and through the seasons, and looks to be made of comparable materials to OEM parts. Once installed, the blades worked exactly as expected. They're not slow or wobbly, and I no longer have any streaks on my front windshield.
Great kit, 10/10 would recommend to anyone! Just make sure you're choosing the correct one for your make and model!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 29, 2026
★★★★★ 5
Great performance and very easy to install.
Size: 26"+16"+12"
I own a 2019 Subaru Crosstrek that I recently purchased. I joined a social media group that is geared to Crosstrek owners to learn more about this model.
I have read quite a few posts on replacing your wiper blades. Many owners reported that you had to use OEM blades from the dealer. Others reported that you could use aftermarket, but needed adapters. Finally, some people stated they just went to Amazon and ordered blades that fit perfectly.
I chose the Amazon route...and am pleased that I did!
I found these blades that stated they fit the Crosstrek. The price was decent, and the reviews were positive, so I ordered a set.
They arrived very quickly and were well packed so that there were no damages.
The set is three blades: the two front and one rear wiper. The quality appeared to be great, with solid frames and heavy rubber blades.
Changing out the front wipers couldn't have been easier! You simply push a button on the blades and they slide right off the wiper arms, then the new blades slides on and snaps into place. I believe it literally took less than two minutes to change out both blades!
The rear was slightly more difficult. You position the old blade perpendicular to the arm and it pops off, then you slip the new blade on and snap it onto the arm.
NO ADAPTERS WERE REQUIRED! Front and rear blades attached perfectly out of the box!
The performance is great as well. We recently had almost three days of steady rain (a very welcome storm for our drought-ridden Colorado!), and they kept up with the rain streak free!
I've only had them on for a month or so, so I can't comment on their long-term durability, but the rubber is holding up very well so far.
I'm not sure if they have these for all makes and models of vehicles, but if they do have yours, I would definitely recommend these for your vehicles!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 17, 2025
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