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Description
Human MRGBP ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against MORF4-related gene binding protein (MRGBP). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of MORF4-related gene binding protein (MRGBP) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human MORF4-related gene binding protein ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | MORF4-related gene-binding protein (MRGBP) is a subunit of the NuA4 histone acetyltransferase complex, which is primarily involved in the transcriptional activation of specific genes through acetylation of ribosomal histones H4 and H2A. This modification may alter ribosome-DNA interactions and promote interactions of the modified histones with other proteins, thereby actively regulating transcription. This complex may be required for the activation of transcriptional programs involved in oncogene- and proto-oncogene-mediated growth induction, tumor suppressor-mediated growth arrest and replicative senescence, apoptosis, and DNA repair. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.5 ★★★★★
Based on 22 reviews
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Product Reviews
★★★★★ 1
Stay Away from this Printer!
I almost never leave negative product reviews, but this one requires the effort to warn others. I would give this item zero stars if that were an option. I have dealt with Epson technical service personnel repeatedly since I originally received this defective printer on February 17 of this year. As a result of those discussions, I received my SECOND replacement printer yesterday. Like the first two printers, this one also does not perform properly. It jams every single time the document feeder is used for scanning. The first page jams every time. None of the online recommended fixes resolves the issue. The first replacement printer could not be connected to my wireless network, so I never even got it set up to test it. The Epson technical service personnel concluded it had a defective network card. The original printer could be set up; but after every print job it completed, it locked up and displayed error code 302626, which I was told by the service personnel indicated a hardware issue of some sort. To say this has been a disappointing experience would be an understatement. I intend never to purchase another Epson product. Epson insisted my only remedy was to continue to receive replacement printers, all of which were refurbished machines. I devoted a total of more than 8 hours to this terrible product. The bright spot in this sorry is that because of my long and strong patronage of Amazon, I was able to receive a refund from and return the machine to Amazon. I will now try an HP model, which hopefully will be a much better experience.
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Reviewed in the United States on March 28, 2026
★★★★★ 4
Write down the serial number of the printer (On the underside of the printer).
This product was packed very, very well. I usually take pictures with my iPhone so that I know how to put products back together if I need to send them back but I was impressed with the way this printer was protected, really thoroughly!
Step through the instructions very carefully. I wouldn't say that is the easiest setup but at least the instructions cover everything you need to know. Some of the features that are very useful - the size (fits on my small file cabinet with room to spare), has ethernet AND wireless and the printed pages tray pops out of the printer automatically when printing starts.
Interesting observations - When loading the ink, do NOT pay attention to the logo onto top of the ink tank cover. It shows the inks from left to right - red, yellow, blue but it's actually (Black) blue, red yellow and there is label on the inside showing the correct sequence and the ink bottles will not fit if you try to put them in the wrong tank. It does save some degree of frustration if you don’t get misled by the logo.
Some bright spark left out an important instruction in the user guide that you will run into when you are installing software. Bear in mind that you have carried the printer to its final location, hooked it up to power and connected cables and installed the ink and waited 11 minutes for the ink to finally finish its whirring and buzzing. You are installing software (maybe the Epson smart panel) and you are told to enter the serial number of the printer as the password. It then informs you that the serial number is on the bottom of the printer or underneath the side panel or on the back panel.
I could not find the serial number in either the side or the back. As far as I could tell, the serial number is only on the underside of the printer so get ready to unplug your cables and don’t tip the printer now that you have loaded the ink! I lifted the printer up (not light) so that I could see the serial number and have my wife write it down. Why there was no instruction to write down the serial number after removing the printer from the box for whichever step it's needed BEFORE going through the entire setup and loaded up with ink seems ridiculous to me. It's certainly pretty annoying to find out you need the serial number from the underside of the printer after completing all the physical installation steps.
The various panels are made of thin plastic and seem flimsy but I do like the way they fit together.
When you run a test print you will be told to place the test print in the scanner so that it can be verified and you might get some errors if you don't place it exactly where it's supposed to be. I am used to positioning the paper with the small edge on the glass right where the lid is raised and the long edge against the edge of the glass near the front. That will get you an error message. There is an arrow (hard to spot) in the corner to tell you where it needs to be exactly, near the hinge of the cover. The instructions will have you go off in another direction if you don't realize that the problem is how you positioned the original on the glass.
On the positive side, the printer worked right away using the ethernet connection to one of the switches in my home network.
I have this printer installed on top of my small filing cabinet in the closet which is next to my desk. I had already installed an AC socket inside the closet for the printer so there was no extra work required. The Ethernet cable fits under the closet doors when they are closed.
I moved the old Epson WorkForce 645 printer upstairs where I have three more Macs. These days it only works well for black ink only which cuts down on cartridge costs. That printer has been a workhorse for sure. It's a 3-in-one also. I expect it will keep on ticking for a while yet which is why I bought a new Epson.
This printer has taken the longest to install of any of my previous printers but I have a photo journal to complete so I wanted to get a decent color printer. Lastly, I recommend that you buy good quality paper to get the best results (I ordered Epson Premium Presentation Paper MATTE (8.5x11inches, Double-Sided, 50 sheets - S041568), Bright White because of my use case).
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Reviewed in the United States on November 2, 2025
★★★★★ 5
Easy to install and works great!
Very happy with this printer. The wireless connectivity works great, the installation app was easy to use, the sprint speed is fine for home use like I do, and it seems to use ink a lot more economically than my old HP Envy. I have just been doing B&W printing, so I can't really comment on the color printing yet. Overall, this has been well worth the price so far.
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Reviewed in the United States on May 10, 2026
★★★★★ 5
Epson ET-3950
I did my research and this is the printer that does everything I need i highly recommend it came well packaged the print quality is great when you have the right settings with the correct paper to print on easy to use and set up. It's worth it .
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Reviewed in the United States on May 12, 2026
★★★★★ 5
Solid performance
Nice color printer for home use. Easy to use. Good scans and good prints on plain white copy paper. Using for wireless printing with mac mini, iphone, and ipad.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 1, 2026
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