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Description
EdU-594 cell proliferation detection kitProduct Specification Usage Before the trial guidelines 1, the corresponding animal experiment, EdU suggested initial dosage of 50 mg kg (50 mu g g), dilute concentration is 0. 5 1 mg mL. Are injected into mice weight 20 g, mg, 1 to 1 mg mL, need injection of 1 mL. 2, for the cells, the recommended 10 microns, EdU work for in vitro culture cell, specific EdU use concentration, incubation time along with the samples and research purpose is different,
Product Specification
| Usage | Before the trial guidelines 1, the corresponding animal experiment, EdU suggested initial dosage of 50 mg/kg (50 mu g/g), dilute concentration is 0.5 1 mg/mL. Are injected into mice weight 20 g, mg, 1 to 1 mg/mL, need injection of 1 mL. 2, for the cells, the recommended 10 microns, EdU work for in vitro culture cell, specific EdU use concentration, incubation time along with the samples and research purpose is different, can be adjusted appropriately. 3, this kit can be carried out about 200 t of 6 orifice EdU experiments, about 2000 t96 orifice or paraffin section EdU experiment, experiment should be calculated according to the sample situation in the process of usage. I. Sample processing 1, the animal experiment (1) EdU injection in animals In mice, can according to the amount of 10-200 mg/kg, the EdU with PBS mixed with a certain concentration, intraperitoneal injection, specific tissues or organs local injections or added to drinking water. The specific dosage is related to the type, weight and mode of use of animals, and can be referred to the relevant literature. Therefore, it is recommended to conduct a certain exploration of the use concentration of EdU for the first use, or directly use the concentration of 50mg/kg for testing. Injection method: the experiment according to the customer, such as abdominal cavity injection, subcutaneous injection, intramuscular, tail intravenous injection, etc., among them with intraperitoneal injection. After 6 h or after the appropriate time was determined according to the specific experiment, the mice were quickly sacrificed, the desired tissue was removed, and frozen or paraffin sections were made according to routine procedures. EdU labeling can also be adjusted by referring to the relevant literature. Small intestinal epithelial cells proliferate rapidly, and positive information can be detected 6 hours after EdU injection in adult mice, which can be used as a positive control for pre-experiments. (2) Slice processing Pre-section treatment: It is best for tissues and organs to be washed to remove residual EdU in blood and tissues and reduce background. Section thickness: 3-10μm is appropriate, too thick section may affect the section background and staining efficiency. Slice post-processing: Paraffin sections were deparaffinized twice with xylene for 15 min, once each with gradient ethanol (100%, 95%, 85%, 75%) for 5 min, and once with deionized water for elution. Frozen section processing: room temperature placed after 30 minutes, fixed 10 minutes, PBS cleaning three times, each time for 5 minutes. 2. Cell experiments (1) cells EdU markers a.with cell complete medium and EdU mother liquor by 2000:1 the ratio of dilution, preparation of a moderate amount of 10 microns EdU medium; b.each hole adding suitable amount of 10 microns EdU medium 2 hours incubation, abandon culture medium (optimal incubation time for 1/10 of the cell cycle) commonly, EdU incubation medium and EdU reaction liquid volume may refer to the table below; c.PBS cleaning cell 3 times, each time for 5 minutes.
a. Cell fixative (PBS containing 4% paraformaldehyde) was added to each well and incubated at room temperature for 15 minutes. The fixative was discarded and washed with PBS for 3 times, 5 minutes each time. b. Each well was decolorized with an osmotic agent (0.5% TritonX-100 in PBS) and incubated for 15 minutes. PBS was washed three times for 5 min each. Second, EdU response
The EdU reaction solution was prepared according to the ratio of PB: CU: AC: 594 chromogen = 855:40:100:5 (the reaction solution was prepared immediately). Each sample add 50-500 mu L EdU staining reaction liquid liquid evenly cover samples (reaction), 15 to 60 minutes at room temperature away from light incubation, abandon the dyeing liquid reaction, PBS wash 3 times, each time for 5 minutes. 3. DAPI staining DAPI(100x) was diluted with PBS at a ratio of 1:100 to make ready-to-use DAPI staining solution. 50-500μL of DAPI staining solution was added to each sample and stained in the dark for 15 minutes, followed by three washes with PBS for 5 minutes each. 4. Take pictures Fluorescence microscope, confocal microscope or whole-film scanner were used to collect images. After staining, the slides were stored in a dark place at 4℃. Above are stained images of 50mg/kg labeled mice for 12 hours |
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| Description | EdU(5-ethynyl-2' -deoxyuridine), also known as 5-ethynyl-2' -deoxyuridine (5-ethynyl-2' -deoxyuridine), is a thymidine analogue with an alkynyl hydroxyl group that is rare in natural compounds and can replace thymidine (thymidine, 5-ethynyl-2' -deoxyuridine) in cell proliferation. thymidine (thymidine) is inserted into the replicating DNA molecule, the ethynyl group on EdU can react covalently with the fluorescently labeled small molecule Azide probe (Azide Alexa Fluor 488, etc.) through the catalysis of copper monovalent ion to form a stable triazole ring. "It is called the Clickreaction, which allows for an efficient and rapid measurement of cell proliferation, especially the percentage of cells in S phase." With traditional immunofluorescence staining (BrdU) method compared with EdU only 1/500 the size of the BrdU antibody, are more likely to spread within the cell, don't need strict sample degeneration (acid solution, pyrolysis, enzyme solution) processing, effectively avoid the damage samples, help in the overall level of tissue and organ observation of cell proliferation, It has higher sensitivity and faster detection speed.Note: AC is a powder, need to add 10mL pure water before use, fully dissolved before use, AC is easy to oxidation failure, after the refrigerator is stored at -20 degrees, if found discoloration, need to replace fresh reagent. In animal experiments, the mass concentration of 100mM EdU mother liquor corresponds to 25mg/mL, which should be converted according to the dosage.
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| Storage Temp. | -20 ° C, valid for 12 months. |
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4.3 ★★★★★
Based on 14 reviews
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Product Reviews
★★★★★ 5
Great fun read
Format: Kindle
Pepper is your typical high school perfectionist and overachiever, something she has control over while her family is falling apart. Her mom is busy keeping their-once small town-fast food restaurant off the ground as an ultra successful chain, and let’s be realistic here, Pepper is as well since she secretly has a large hand in the social media aspect. Her sister is away at college and has a strained relationship with their mom and their dad is back in their hometown managing the original Big League Burger.
Jack is the son of a rival burger shop, twin, and class clown. He usually prefers to fly under the radar where most things are concerned, perfectly content with hiding away developing his apps. Apps like the one being banned at school. The one he designed, for students only, to anonymously post under the guise of an animal. That is, until Big League steals his family’s grilled cheese recipe. Only then does he find himself locked in a twitter war with Pepper. What neither realize is that they’re falling for each other behind animal faces on his app while the twitter war goes viral.
I enjoyed this book, but I didn’t love it quite as much as the other work I’ve read by Emma. I found the beginning a little hard to get into and I wasn’t a huge fan of how the two were forced to spend time together, but after four chapters or so I was hooked and didn’t want to put it down. The banter we love is there between the characters and they’re almost sickeningly cute together. Though I have to admit, I love the PepperJack ship. The name is too perfect. I loved Jack’s opinion of being on Twitter, yelling into a void. Just a great, fun read overall.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 27, 2023
★★★★★ 4
4 star : Cute, Clean, YA romance
Format: Paperback, Format: Paperback
*Tweet Cute*
🏊🏻♀️🤽🏻♀️🐺📱🧁🗽
⭐️⭐️⭐️⭐️
**Synopsis:**
Pepper. Captain of the swim team, amazing baker, & secret runner of her Mom’s fast food place Big League Burger’s. Jack. Stuck in the shadow of his twin, works his family’s deli, and truly hates that Big League Burger’s grilled cheese recipe is the same as his grandmas. Jack takes matters into his own hands & takes it to Twitter. With pepper & Jack secretly in charge of the Twitter accounts, it quickly turns into a Twitter war! As they are fussing on Twitter & in real life, little do they know, they know more about each other then they think. They anonymously message each other on an anonymous app (that Jack created) for students at their school. Blue bird & Wolf get along quite well as Pepper & Jack continue their fighting. How will the worlds cross? Will the lines start blurring from hate to love?
**Personal thoughts:** this was a very cute book! If you ever read Alex, Approximately or watched Anonymously Yours… very similar! Absolutely loved the banter & connections made. I loved how the author included the tweets in the books & it wasn’t just a back & forth dialogue. I wasn’t obsessed but found it very cute & adorable 🥰
**Spicy level:** 🌶️/5 | minimal romantic gestures
**Trigger warnings:** N/A can’t remember any
⭐️⭐️⭐️⭐️
Instagram: @readswithtayee
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 1, 2024
★★★★★ 5
Clear, Practical Guide for Entry-Level to Intermediate Multifamily Underwriting
Format: Kindle
As someone learning multifamily underwriting from the ground up, this book was incredibly helpful. It breaks down complex underwriting concepts into a logical, step-by-step process that actually mirrors how deals are analyzed in the real world.
What I appreciated most is that the author doesn’t just show formulas — he explains why assumptions matter, where risk hides, and how underwriting decisions affect outcomes. The sections on operating expenses, stabilization, financing, and sponsor economics were especially valuable and gave me a much clearer framework for analyzing multifamily properties with confidence.
This book feels less like theory and more like a practical guide you can follow alongside a real underwriting model. I’d highly recommend it to anyone getting started in multifamily underwriting or looking to tighten up their analysis process.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 20, 2025
★★★★★ 5
Underwriting Multifamily
Format: Kindle
This book deserve a five star rating. It uses practical examples easy to follow. This well written book easy to understand. I recommend this book to any real estate investor.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 20, 2024
★★★★★ 5
Must Have Book for Investors or Sponsors/GPs wanting to Invest in Multifamily Properties!
Format: Paperback
If you understand the math and numbers associated with investing in real estate, this book will help you underwrite any multifamily property. Not only does Robert's book discuss how to gather the information needed for underwriting, he goes into detail on how sponsors can manipulate the numbers. This is a great book for knowledge on the pros and cons of varied partnership structures and how each can create an alignment of interest between limited partners (LPs) and general partners (GPs). Overall, this is a great easy read book.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on September 13, 2020
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