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Description
MAP2 Recombinant Rabbit mAb (S-3110-72)Product Specification Host Rabbit Antigen MAP2 Synonyms Microtubule associated protein 2; MAP 2; Mtap2; Map2 Immunogen Synthetic Peptide Location Cytoplasm, Cytoskeleton Accession P20357 Clone Number S 3110 72 Antibody Type Recombinant mAb Isotype IgG Application IHC P, IF Reactivity Ms, Rt Purification Protein A Concentration 0. 5 mg ml Conjugation Unconjugated Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0. 05% BSA, 0. 03% Proclin
Product Specification
| Host | Rabbit |
| Antigen | MAP2 |
| Synonyms | Microtubule-associated protein 2; MAP-2; Mtap2; Map2 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cytoskeleton |
| Accession | P20357 |
| Clone Number | S-3110-72 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P, IF |
| Reactivity | Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:250 | Ms, Rt |
| IF | 1:100 | Ms, Rt |
Background
MAP2 (microtubule-associated protein 2) is a ~280 kDa heat-stable phosphoprotein expressed almost exclusively in neurons, where it binds along the sides of microtubules in dendrites, stabilizing them and determining dendritic caliber and branching by spacing microtubules apart; its single tandemly repeated 31-amino-acid microtubule-binding domain is regulated by phosphorylation, and proteolytic cleavage of the projection domain during excitotoxic or ischemic insults produces 40–50 kDa fragments that rapidly depolymerize dendritic microtubules, contributing to neuronal death, while antibodies to these fragments serve as widely used selective markers of dendritic cytoarchitecture in neurohistochemistry.
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