SKU: 53722538169

Protein L Donor Beads

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Description

Protein L Donor BeadsProduct Specification Stability & Storage Store at 2 8C away from light; product shelf life is 12 months. Background Homogeneous Immuno Chemiluminescence Assay (HICA) is a homogeneous immunoassay method based on energy transfer between donor beads and acceptor beads in close proximity, resulting in luminescence. Donor beads recognize protein 1 (Tag1 label), while acceptor beads recognize protein 2 (Tag2 label). When protein 1 binds to protein 2, the

Product Specification


Stability & Storage

Store at 2-8°C away from light; product shelf life is 12 months.

Background

Homogeneous Immuno Chemiluminescence Assay (HICA) is a homogeneous immunoassay method based on energy transfer between donor beads and acceptor beads in close proximity, resulting in luminescence.

Donor beads recognize protein 1 (Tag1 label), while acceptor beads recognize protein 2 (Tag2 label). When protein 1 binds to protein 2, the distance between the beads becomes less than 200nm. Upon excitation at 680nm, the donor beads generate singlet oxygen, which diffuses to the acceptor beads. The acceptor beads then undergo a redox reaction, emitting light at 615nm. The signal intensity is directly proportional to the strength of the protein interaction.

This product features a simple operation process, requiring no washing, and offers fast results with high sensitivity. It is capable of detecting weak interactions.

Components

Specification

Fill Volume

250 μg

50 μL

5 mg

1 mL

25 mg

1 mL x 5

Protocol

[Required Reagents]

Name

Catalog Number

Protein L Donor Beads

UA086111

Streptavidin Acceptor Beads

UA086090

Universal Buffer 1

UA086113


 

 

[Detection Procedure for Reference]

Detection Procedure

Detection Procedure 1 (37℃Rapid Detection)

Detection Procedure 2 (Room Temperature Detection)

​Step 1:

4μL hFC tag-M1 +4μL Biotin-M2+ 6μL Protein L Donor Beads，Protect from Light/Green Light

4μL hFC tag-M1 +4μL Biotin-M2+ 6μL Protein L Donor Beads，Protect from Light/Green Light

Incubation

37℃ Shaking Incubation 20 minutes，Protect from Light/Green Light

Room Temperature Incubation 60 minutes，Protect from Light/Green Light

​Step 2:

Add 6μL Streptavidin Acceptor Beads，Protect from Light/Green Light

Add 6μL Streptavidin Acceptor Beads，Protect from Light/Green Light

Incubation

37℃ Shaking Incubation 10 minutes，Protect from Light/Green Light

Room Temperature Incubation 30 minutes，Protect from Light/Green Light

Readout​

Instrument Reading

Instrument Reading


 

 

[Performance Validation]

•Sample Preparation:

Use Universal Buffer 1 to pre-dilute Biotinylated Human IgG (Bio-hIgG) to 15μg/mL (100nM) as stock solution, then perform gradient dilution according to the following scheme:

ID

Final Concentration (nM)

Universal Buffer 1

Volume (μL)

High Concentration Addition

Volume (μL)

C12

1.0E+01

210

90μL Stock Solution

C11

3.0E+00

210

90μL C12

C10

1.0E+00

180

90μL C11

C9

3.0E-01

210

90μL C10

C8

1.0E-01

180

90μL C9

C7

3.0E-02

210

90μL C8

C6

1.0E-02

180

90μL C7

C5

3.0E-03

210

90μL C6

C4

1.0E-03

180

90μL C5

C3

3.0E-04

210

90μL C4

C2

1.0E-04

180

90μL C3

C1

0

180

/


 

•Detection Reagent Preparation:

Name

Preparation Concentration

Diluent

Protein L Donor Beads

25 μg/mL

Universal Buffer 1

Streptavidin Acceptor Beads

25 μg/mL

Universal Buffer 1


 

•37℃ Incubation Mode Detection Results:

Max Signal: 450369

Min Signal: 616

EC50= 0.082 nM

•Room Temperature Incubation Mode Detection Results:

Max Signal: 191706

Min Signal: 279

EC50= 0.059 nM

Guidelines

1. This experiment is light-sensitive; ensure all operations are performed in the dark. It is recommended to conduct preparation, sample addition, and incubation steps under green light (illuminance below 100 LUX). 2. This product is compatible with multi-functional microplate readers equipped with an Alpha detection module. 3. Vortex thoroughly before use. Alternatively, briefly centrifuge (2000×g, 5–10 seconds) to ensure complete retrieval. 4. It is recommended to use the company's accompanying dilution buffer for reagent preparation and sample dilution. If additional components are required, they can be directly added to this dilution buffer. 5. To ensure comparability of experimental data across different batches, strictly control incubation temperature and duration. 6. Avoid generating bubbles during sample addition.
Shipping Notes
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Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
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SKU: 53722538169

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4.6 ★★★★★
Based on 28 reviews
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Product Reviews
J
Verified Purchase
Jennifer Mazariegos
San Leandro, US
★★★★★ 4
Great product
Size: 1 Count (Pack of 1)
I use it at every fill up. Works great
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 8, 2026
D
Verified Purchase
Donna Garrett
Los Angeles, US
★★★★★ 5
TDI Seems To Like This Stuff. A Must For US D2 Fuel.
Size: 1 Count (Pack of 1)
After purchasing our 2012 VW TDI diesel at 140K miles I started researching best practices to keep these little engines happy. What I read was that the US has some of the crappiest quality D2 diesel on the world stage. Even Canada's diesel is higher cetane and lubricity rated than the States not to even mention Europe which has WAY higher standards than us. The weak point of these TDI engines seems to be the high pressure fuel pump (HPFP) that is crank driven to enormous pressure for the injectors to to their thing. If these HPFP parts fail on common rail engines they can literally grenade and throw shards of metal everywhere within the downstream fuel system ensuring a VERY expensive repair if not destroying the engine altogether. The lubricating property of the fuel plays an important part in keeping these pumps working properly. There even seems to be some data supporting Canadian operated TDIs have fewer failure rates of the HPFP than stateside fueled vehicles although nothing had been officially published to my knowledge. Sulpher in the diesel had beneficial lubricating properties but was bad for the environment and subsequently removed from D2 by the EPA much like leaded gasoline of yesteryear. So the second tank I ran in my TDI was Hot Shot's EDT. I can't comment on the comparative difference as I ran this from almost day one. What I like is the claimed increased lubricity and cetane boost that is supposed to bring our crappy D2 up to reasonable levels the rest of the world enjoys. Fuel economy seems fine but playing with a low spun turbo and the subsequent torque is sometimes too irresistible to give fuel economy a fair chance. Besides, I need to blow out all that accumulated carbon :) I like the fact that the Hotshot's EDT seems more concentrated and affordable than the competition. 1oz for a tank is almost a performance treatment for my TDI. It's a negligible cost for a fill up and I hope they keep it that way. My dislike might be the one thing other people love about this product and that's the bottle. The bottle is meant to be squeezed to get the measured amount in the top fill area so measuring is easier. My concern is the filler neck is not long enough to cram into the filler to avoid spilling without a funnel and diesel doesn't do many favors to paint. My workaround was to save the empty bottles from other gasoline treatments and use a syringe to fill 1oz amounts of EDT and place in the trunk of the car in double plastic bags. Seems to work fine although the kitchen was probably not the best place to do this since it doesn't smell like flowers. Next purchase will likely be the bulk bottle. It's kind of a shame we actually need this kind of protection now since the US fuel standards are almost non-existent. Even B1 blended bio diesel would be a huge improvement in lubricity but not available where I live. Thanks Hotshot's...Good stuff!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 31, 2020
S
Verified Purchase
SCOTT
Alexandria, US
★★★★★ 5
Recommended by John. So far great additive for the CP4
Size: 16 Fl Oz (Pack of 2)
I have a ‘22 6.7 Powerstroke w/27k and pull heavy trailers in FLA. I’m worried about the (disco party). John I believe from the Ford Dealer in Hereford, Tx recommended this. I run 2 oz per fill and will see what happens. I’ve got Five fill ups on it now and seems to help responsiveness. Proof will be in the pudding. So far I’m pleased
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 9, 2026
R
Verified Purchase
ReevesRacing
Louisville, US
★★★★★ 5
Quieter and more mpg in TDI
Size: 1 Count (Pack of 1)
Didn't really believe the hype. Did a little research and watched Project Farm and decided to try. Im not a snake oil guy....but I will say my 2013 common rail TDI is quieter and I get a tad more fuel mileage. Does it pay for itself? Well, it does get better mpg, but I feel the reduction in noise most likely relates to less wear and tear and thats good enough for me. Im only using 1oz per fill up (about 14 gallons) so it lasts a very long time. I get 40mpg and im not light footed.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 2, 2026
J
Verified Purchase
James Jay
Lowell, US
★★★★★ 5
It's a spark plug
What i needwd
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 27, 2026

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