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Description
FITC Mouse Anti-Rat CD43 Antibody (S-R632)Product Specification Host Mouse Antigen CD43 Synonyms Leukosialin; Leukocyte sialoglycoprotein; Sialophorin; W3 13 antigen; Spn Location Membrane, Cell projection Accession P13838 Clone Number S R632 Antibody Type Mouse mAb Isotype IgG1,k Application FCM Reactivity Rt Positive Sample SD Rat splenocytes Purification Protein G Concentration 0. 05mg ml Conjugation FITC Physical Appearance Liquid Storage Buffer PBS, 25% Glycerol, 1% BSA, 0. 3% Proclin
Product Specification
| Host | Mouse |
| Antigen | CD43 |
| Synonyms | Leukosialin; Leukocyte sialoglycoprotein; Sialophorin; W3/13 antigen; Spn |
| Location | Membrane, Cell projection |
| Accession | P13838 |
| Clone Number | S-R632 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Rt |
| Positive Sample | SD Rat splenocytes |
| Purification | Protein G |
| Concentration | 0.05mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Rt |
Background
CD43, also known as leukosialin or sialophorin, is a type I transmembrane glycoprotein abundantly expressed on the surface of most hematopoietic cells, with the exception of erythrocytes and resting B cells. It has a heavily glycosylated extracellular domain and a cytoplasmic tail. CD43 plays multiple roles in immune responses, including regulation of T-cell activation, proliferation, and cytokine production. It also interacts with ERM proteins (ezrin, radixin, moesin) via a specific sequence in its cytoplasmic tail, which is important for its function in cell adhesion and migration. Additionally, CD43 has been implicated in various biological processes such as leukocyte migration, immune cell signaling, and even tumor progression. Its expression and function can vary depending on the cell type and the specific immune context.
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