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Description
α-tubulin Mouse mAb (S-364-23)Product Specification Host Mouse Antigen tubulin Synonyms Tubulin alpha 4A chain, Alpha tubulin 1 Immunogen Synthetic Peptide Location Cytoplasm, Cytoskeleton Accession P68366 Clone Number S 364 23 Antibody Type Mouse mAb Isotype IgG1,k Application WB, IHC P, ICC, ICFCM Reactivity Hu, Ms, Rt, PIG, Zf Predicted Reactivity Bv, SeUr, Pg, Lob, Cz, Pl, Fs, Dr, Ar, Xe, Hm, Av Purification Protein G Concentration 2 mg ml Conjugation Unconjugated Physical
Product Specification
| Host | Mouse |
| Antigen | α-tubulin |
| Synonyms | Tubulin alpha-4A chain, Alpha-tubulin 1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cytoskeleton |
| Accession | P68366 |
| Clone Number | S-364-23 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | WB, IHC-P, ICC, ICFCM |
| Reactivity | Hu, Ms, Rt, PIG, Zf |
| Predicted Reactivity | Bv, SeUr, Pg, Lob, Cz, Pl, Fs, Dr, Ar, Xe, Hm, Av |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| WB | 1:1000~1:5000 | |
| IHC-P | 1:500 | |
| ICC | 1:1000 | |
| ICFCM | 1:2000 |
Background
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Tubulin α- and β-subunits have molecular weights of ~ 50 kDa and are 36%–42% identical and 63% homologous. Both tubulin subunits bind guanine nucleotides. The binding to α-tubulin at the N-site is nonexchangeable, while the binding to β-tubulin at the E-site is exchangeable. Nucleotide in microtubules does not exchange with the solution, except for terminal subunits at microtubule ends.
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