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Description
LR3-IGF-I Protein, Human/Porcine/BovineProduct Specification Species Human Synonyms LR3 Insulin like Growth Factor I, MGF, IGFI, IGF1A, IGF, IBP1 Accession P05019 Amino Acid Sequence Gly49 Ala118 (Glu51Arg) Expression System E. coli Molecular Weight 10 kDa (Reducing) Purity >95% by SDS PAGE. Endotoxin <0. 1EU g Conjugation Unconjugated Tag No Tag Physical Appearance Lyophilized powder Storage Buffer 50mM HAc Reconstitution Reconstitute at 0. 1 1 mg ml according to the size in ultrapure
Product Specification
| Species | Human |
| Synonyms | LR3 Insulin-like Growth Factor-I, MGF, IGFI, IGF1A, IGF, IBP1 |
| Accession | P05019 |
| Amino Acid Sequence | Gly49-Ala118 (Glu51Arg) |
| Expression System | E.coli |
| Molecular Weight | 10 kDa (Reducing) |
| Purity | >95% by SDS-PAGE. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | 50mM HAc |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | Endocrinology. 1999 Oct;140(10):4699-705. |
Background
IGF-I is a well-characterized basic peptide secreted by the liver that circulates in the blood. It has growth-regulating, insulin-like, mitogenic activities. IGF-I is a growth factor that has a major, but not absolute, dependence on somatotropin. It is believed to be mainly active in adults in contrast to IGF-II, which is also a major fetal growth factor. Human Long R3 Insulin-like Growth Factor-I (rhLR3-IGF-I) contains an 83 amino acid analog of human IGF-I. Compared to the complete human IGF-I sequence, an addition of the rhLR3-IGF-1 includes the substitution of an Arg for the Glu at position 3 (hence R3) and a13 amino acid extension peptide at the N-terminus. An enhanced potency is due to the markedly decreased binding of human Long R3-IGF-I to IGF binding proteins which normally inhibit the biological actions of IGFs.
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