Human IL-2 Kit (HICA)

Principle of the Assay:

This kit utilizes a homogeneous immuno chemiluminescence assay (HICA) based on a double-antibody sandwich method for the detection of cytokine concentrations. The operation is simple and requires no washing steps.

The detection system includes two types of microspheres: receptor microspheres conjugated with antibody 1 targeting the protein of interest, and donor microspheres conjugated with streptavidin, along with biotin-labeled antibody 2. During the reaction, the target protein binds with the antibodies and microspheres to form an immune complex, bringing the two types of microspheres into close proximity. When the distance between donor and receptor microspheres is less than 200 nm, singlet oxygen generated by photoexcitation of the donor microspheres can transfer to the receptor microspheres and trigger chemiluminescence. Conversely, if the target protein is absent, the microspheres remain too far apart, and no signal is produced.

By measuring the intensity of the chemiluminescent signal, quantitative analysis of the target protein can be achieved. This method offers advantages such as simplicity, rapid reaction, and high sensitivity.

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Note: It is recommended to use microplates (384 or 96 wells, white, shallow wells) for the assay.