Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 30 - Sep 4
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Mouse ALDH ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as an anticoagulant and centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Pre-Test Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 10 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an acetaldehyde dehydrogenase (ALDH) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of acetaldehyde dehydrogenase (ALDH) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Acetaldehyde dehydrogenase | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Acetaldehyde dehydrogenase (ALDH), a type of aldehyde dehydrogenase, catalyzes the oxidation of acetaldehyde to acetic acid. Alcohol dehydrogenase in the liver oxidizes ethanol (a component of alcohol) to acetaldehyde. The resulting acetaldehyde serves as a substrate for further conversion to harmless acetic acid (a component of vinegar) under the catalysis of ALDH. Aldehyde dehydrogenases are polymorphic enzymes responsible for oxidizing aldehydes to carboxylic acids, which are metabolized by the body's muscles and heart after leaving the liver. These enzymes exist in three distinct classes in mammals: class I (low Km, located in cell membranes), class II (low Km, located in mitochondria), and class III (high Km, such as those expressed in tumors, stomach, and cornea). Within these three classes, there are constitutive and inducible forms. ALDH1 and ALDH2 are the most important aldehyde oxidases, both tetrameric enzymes composed of 54 kDa subunits. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, cell culture supernatant |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.2 ★★★★★
Based on 24 reviews
Sort
Product Reviews
★★★★★ 4
Pillowriffic
Color: White, Size: Queen (Pack of 2), Color: White, Size: Queen (Pack of 2)
These pillows are exactly as described, I would say they are softer than I expected but still very good. They come compressed so it will take an hour or so for them to fill up so you will want to open these before bedtime. All in All if you are looking for a reasonably priced pillow that will last a couple of months I would recommend these.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 7, 2026
★★★★★ 5
Great buy
Color: White, Size: Queen (Pack of 2)
I was very skeptical about buying pillows online. I am pleasantly thrilled with these pillows. They are great quality. They maintain their shape by simply shaking occasionally if needed. The fabric is really soft to the touch. They don't go flat after a few uses. I've had them for almost 6 months. I waited to review to make sure they didn't disappoint.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 6, 2026
★★★★★ 5
LOVE THEM!!! SAVED MY NECK!!!
Color: White, Size: Queen (Pack of 2)
Hallelujah!!! After spending so much money on pillows that didn't work for my aching neck and shoulders, I finally found pillows that works for me and I am SOOOOOO excited. I had my 1st night of comfortable sleeping after years of restless, painful nights. These pillows are the BOMB! I kid you not. I have spent hundreds of dollars on pillow to no avail. I mean, the expensive hotel pillows that were suppose to make you feel as if you're sleeping on clouds made my neck stiff and painful. Let's not talk about that "infamous pillow: advertised on the infomercial that was suppose to be the #1 pillow to fight against neck and back pain. Shhhh, we won't say the name but uh....TRASH. Ok, I'm back. Lost it there for a moment thinking of all the money I wasted on all those pillows that claimed to be "THE PILLOW." LOL..what a joke. So, anyway back to what I was saying about this pillow beauty. The very 1st night I slept on it, I'll admit I was skeptical when I laid down. Scared I was going to wake up with a sore neck and headache to go along with it. I tore the package open, let the pillow inflate, fluffed them with my hands and allowed them to sit for a few hours. I didn't wait the allotted time suggested because I was too anxious to try them and it was almost my bed time. So, excitedly I jumped in bed, laid my head down on the pillow and immediately thought, "oh no, these are too soft. This will not work." I just knew I would wake up to a sore neck. Cautiously, I laid down and waited to drift off, dreading waking up the next morning with a stiffer. (LOL) To my surprise I actually slept all night too. I usually would wake up like a thousand times fighting with repositioning the pillow to find my comfort zone. WOW, not that night. I slept like a baby and wait for it.....I woke up the next morning with NO PAIN! Hallelujah! I couldn't believe it. I was literally pain free in my neck and did not have a headache from the strain on my neck, shoulders and back. Man, is this really happening, I asked myself. This just can't be real. Wake, wake girl. You are dreaming. But nope. I was fully awake and with a pain free neck and shoulders. I jumped up and started getting ready for my day. I finally found the pillow that I wish I had found years ago. Could have saved so much $ from going down the drain. I am SO thankful to all the reviews that I read that shared their positive experience and encouraged me to purchase for myself. I am SOOOO GLAD I took a chance and brought these little beauties. They saved my neck and my sanity. Thank you EIUE Hotel Collection for inventing the pillow that is reasonably priced and that does the job it's intended to do. Allow people to have a good nights rest WITHOUT neck, shoulder and back pain. Your Rock! This is a real review and I am a real person.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 15, 2025
★★★★★ 3
Flat
Color: White, Size: Queen (Pack of 2)
They are long pillows but definitely flat, I tried and tried to fluff them up and they are about like the $3 Walmart pillows. I will be returning these
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 28, 2026
★★★★★ 5
Holy Wow!
Color: White, Size: Queen (Pack of 2)
These pillows are so fluffy and material is really soft. They came vacuumed packed. I thought well this will be a return for sure. Followed the directions and placed them in my dryer for 15 minutes. Could not believe how big and fluffy they were. My company always ask for the link and I have ordered them for family members and for myself 4 times. Mine I’ve had over a year and have kept their shape and fluffiness. If you like a fluffy pillow…this is the one. They are the best!!!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 7, 2026
recommand products
Cisco Intel Xeon Gold 5220S Octadeca-core (18 Core) 2.70 GHz Processor Upgrade
2237.68
Goulds 7L152 - LOWER S.SLEEVE-70L
135.71
Raritan SecureLock Standard Power Cord
92.36
Goulds 7K620 - CABLE GUARD
66.05
BTI H6Y88AA#ABA-BTI AC ADAPTER 19V 45W H6Y88AA, H6Y88AA#ABA, H6Y88UT, H6Y88UT#ABA ELITEBOOK 1040 G1, G2, 1020 G1, 800 G1, G2, G3 Series 700 G1, G2, G3, 600 G1, G2, HP 215, 240 G2, 245 G2, 250 G2, and 255
76.09