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Description
Human COL1A1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Sensitivity | 0.05 ng/mL | |||||||||||||||||||||||||||||||||
| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Collagen Type I Alpha 1 (COL1A1). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Collagen Type I Alpha 1 (COL1A1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Collagen Type I Alpha 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Type I collagen alpha 1 (COL1A1), also known as alpha-1 collagen, is a protein encoded by the COL1A1 gene. It is a fibrillar collagen found in most connective tissues, including cartilage. Collagen is a protein that strengthens and supports many tissues in the body, including cartilage, bone, tendons, skin, and the white part of the eye (sclera). The COL1A1 gene produces a component of type I collagen, called the proviso α1(I) chain. This chain combines with another proviso α1(I) chain and a proviso α2(I) chain (produced by the COL1A2 gene) to form the type I procollagen molecule. These three rope-like procollagen molecules must be processed by enzymes outside the cell. Once processed, they arrange themselves into long, thin fibers that cross-link with each other in the space surrounding the cell. The result of these cross-links is the formation of very strong, mature type I collagen fibers. The functions of collagen include rigidity and elasticity. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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4.6 ★★★★★
Based on 21 reviews
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Product Reviews
★★★★★ 3
Decent for the price. Instructions suck but most people should be able to figure it out. More below.
Color: Black, Size: 6 Panel, Color: Black, Size: 6 Panel
SUMMARY:
3 stars from me because it's firmly average. It's fine for the price. Assembly and materials are alright but I can see some caveats depending on your circumstances. Assembly instructions do a subpar job of pointing out some details.
ASSEMBLY INSTRUCTIONS:
Maybe it's because I'm autistic and/or building model kits and assembling stuff is my jam, but I honestly thought people were exaggerating when they were complaining about the instructions and I'd be able to flex my ~superior assembly skills.~ I was wrong! These instructions genuinely suck, and whoever is responsible for making them should be ashamed.
The instructions do a poor job of calling out some details regarding orientation of parts, and some of the images do not actually match the physical parts.
For example, it does not really outline the assembly of the end panels clearly, and I can see someone accidentally using the wrong poles. I've drawn over a photo to show what you should do to try and make it clearer. Additionally, the manual shows a flathead screw for bolting the feet into position, but the actual screw is not a flathead. It also does not point out the counterbore, so if you aren't paying attention you may put the foot pads onto the wrong side of the foot. It's also missing the fact that you need to use another one of the plastic pieces when you finish assembling a panel.
ACTUAL ASSEMBLY & MATERIALS:
To their credit, all of my bags were clearly labeled. The assembly process wasn't difficult. It's mostly just tedious and requires a fair amount of space. I was able to assemble it by myself without any real difficulty. However, the way it's assembled means two things.
One, the fabric parts aren't removable without disassembly. So if you want to use this in an environment where they would require cleaning, I would seriously recommend looking for another option. Or, you could buy this just to use the frame pieces and then somehow buy or make your own fabric pieces designed to be removable with velcro or something.
Two, because of the materials I really don't have a lot of faith in this thing surviving disassembly and reassembly. Like a lot of sorta-cheap-but-convenient furniture, it uses those spring-button connections and plastic inserts with self-tapping screws. Those things are not really meant to be disassembled and rethreaded. It also relies a lot on the tension of the poles and the fabric to keep everything rigid and squared, which I think puts a lot of pressure on the aforementioned buttons, plastic inserts, and the hollow metal rods. So I feel like that will also cause issues with disassembly and reassembly.
Basically once this thing is assembled, it's not really meant to be disassembled. The best you can do is spot-clean the fabric if you need to.
Speaking of the fabric, I didn't see any labels on them or anything in the manual that says what they are, but they feel like some kind of polyester. They generate static electricity pretty easily, and pet hair and debris sticks easily. So that's another downside of them not being easily removable.
For the most part it does seem pretty stable. The poles seem to be pretty uniform in length so they're all making contact with my floor. Obviously this isn't structural so it shouldn't be supporting anything, but the two main feet seem to be doing fine with keeping this thing upright.
CLOSING THOUGHTS:
Really, it's fine for what it is, but it could be better in a lot of little ways and the substandard quality of the instructions just seems unprofessional to me, which is why I'm being so harsh with my rating. Depending on your needs and environment you may want to consider a different option. Preferably one that is made to be disassembled with better materials, and/or one with fabric pieces made to be removed easily.
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Reviewed in the United States on May 28, 2023
★★★★★ 1
Not as it seems
Color: Black, Size: 6 Panel
these are awful... Each individual panel is fine, but when you put it together it can barely stand up, and the clips that hold it together keep popping off. Steer clear of this item.
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Reviewed in the United States on April 7, 2026
★★★★★ 5
As described
Color: White, Size: 6 Panel
Works great in my clinic
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Reviewed in the United States on May 17, 2026
★★★★★ 5
Stable, solid, and versatile!
Color: Black, Size: 8 Panel-176'' Wide, Color: Black, Size: 8 Panel-176'' Wide
Exceeded my expectations. Easy, but tedious to assemble. That said, it’s solid, well made, and will last. You can stand it up in a straight line without concern it will fall over. No need to fold it like an accordion for stability. Helped me separate my garage gym from the kids bikes, shelving, paint, tools, and typical garage stuff.
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Reviewed in the United States on November 13, 2025
★★★★★ 5
Awesome !!
Color: Black, Size: 8 Panel-176'' Wide
This Divider is absolutely as advertized and works perfectly !! The quality is outstanding and the set up is easy to understand. Perfect !!
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Reviewed in the United States on March 24, 2026