Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 28 - Sep 2
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Mouse dop1a ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against protein dopey-1 (dop1a). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of protein dopey-1 (dop1a) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Protein dopey-1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | DOP1A (DOP1 Leucine Zipper-Like Protein A) is a protein-coding gene. Diseases associated with DOP1A include immunodeficiency 23 and hyperimmune disease. An important homologue of this gene is DOP1B. DOP1A may be involved in protein trafficking between the late Golgi apparatus and early endosomes. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.1 ★★★★★
Based on 29 reviews
Sort
Product Reviews
★★★★★ 5
I am hard to please...especially expensive products...
K...So I have this 26 ft. sailboat. I have all the professional equipment you can use to restore fiberglass shine. However, a 15 year old sailboat's topside will usually have pitting that you COULD sand down and shine.. but this is a risky step as you think the gelcoat and if you go through, now you have an eyesore of an entirely different nature... My only problem with trying this was the idea that it would fade out shortly and possibly yellow. However, a bigger concern is continued breakdown of the glass surface. An over-riding concern. Wax on a surface that is pitted is usually short lasting and does shine well..good for 3 months, maybe. I bit the bullet and bought this after looking at it's competitors. BUY THE KIT, NOT JUST THE POLIGLOW. The cleaner worked better than any other I have bought. I don't know why. It just did. The scrubbing sponge is perfect for the job. I used a smaller dishwashing scrubber in tight spaces..it seriously was inadequate. This kit was made right, I'm thinking. Following the directions to apply per the bottle. I was skeptical about using a damp mit. I have done the entire cockpit area of the boat at this point with 6 coats and used maybe a half an inch of the product. And the boat looks absolutely AMAZING. Will this yellow? Will it last a year? IDK, but other reviews suggest positive experiences on both counts. Best of all, this will HALT the ongoing beating the topsides takes. (On another note, don't use this on nonskid. It's slick..just my opinion, maybe no more slick than new nonskid. But there is a really great product if you search "nonskid fiberglass surface restorer." Expensive but works.) Best description I can give you on how this will turn out is .. you know how good a surface that is aged looks when you wet it? That..but it stays. Yes, you will need 4-6 coats. But it dries very quickly and still doesn't use much product. At this performance level, the price was worth it. And I am frugal beyond belief. I would recommend that you do professionally buff any fiberglass surface with a good medium one step cutting compound and a professional buffer before you apply this to get best results. DO NOT USE THIS ON SURFACES THAT ALREADY ARE SHINY. It says so right on the kit. I also agree your smoothest outcome will come at room temp vs applying int the sun or significant heat. It dries quickly and doesn't even out as well before drying. Still, it will look great.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 8, 2016
★★★★★ 5
YOU WILL LOVE IT!!!
This is the best product I’ve ever used on our boat! We have a 26-year-old Tige wakeboard boat that had heavy oxidation and water spots. In addition to a heavily faded gel coat. I spent a few hours working on it and it completely restored our boat! I got the recommendation from my father in law after he used it on his RV. The results speak for themselves. I highly recommend this product!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 21, 2026
★★★★★ 5
Great Product!
Does what it says. It made my 20-year-old RV look like new!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 4, 2026
★★★★★ 5
Wish I Had Used This Sooner – Stop Debating and Just Buy It!
Wish I Had Used This Sooner – Stop Debating and Just Buy It!
After a lot of debate, extensive research, and way too many hours spent buffing my RV with other products that never fully delivered the results I was after, I finally tried Poli Glow—and I am extremely happy I did. My only regret is not using it sooner instead of letting other people's opinions talk me out of it.
The difference in how my RV looks after applying Poli Glow is genuinely impressive. The high-gloss finish it lays down on the fiberglass is deep, clean, and long-lasting in a way that the other products I tried simply could not match. The application process with the included mitt is straightforward and far less labor-intensive than traditional buffing and polishing methods, which alone makes it worth switching to. The hydrophobic properties mean water beads and rolls off beautifully, and knowing the UV protection is working to shield the fiberglass from the relentless Las Vegas sun gives real peace of mind for long-term appearance and protection.
For anyone who has been going back and forth on whether to try Poli Glow or sticking with whatever you've been using—stop debating, stop listening to everyone else's opinion, and just try it. The results speak for themselves and I won't be using anything else on my RV going forward. Highest possible recommendation.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 13, 2026
★★★★★ 5
Works Great, A Lot of Elbow Greese, but Worth the Effort!
Transformed our motorhome exterior . It looks new, brighter, and no haze! It helped to watch the instruction videos on YouTube.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 14, 2026
recommand products
Kala KA-SPRK-GOLD Concert Ukulele (Gold Sparkle)
103.00
GREENWOOD VILLAGE, I Love City's, White, Women's Short Sleeve Round Neck T-shirt 00012
20.00
LAKE ST.LOUIS, I Love City's, White, Women's Short Sleeve Round Neck T-shirt 00012
20.00
Upland Disc Golf Ladies All-Weather 3-in-1 Jacket
89.95
BEMIDJI, I Love City's, White, Women's Short Sleeve Round Neck T-shirt 00012
20.00