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Description
Human TLR2 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Saliva Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH=7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results).
Product Specification
| Usage | Required experimental equipment:
1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Saliva Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH=7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Gently wash adherent cells with pre-chilled PBS, then trypsinize and collect the cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash the collected cells three times with pre-chilled PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Collect the supernatant for analysis, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Toll-Like Receptor 2 (TLR2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the Toll-Like Receptor 2 (TLR2) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Toll Like Receptor 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Toll-like receptor 2, also known as TLR2, is a protein encoded by the TLR2 gene. TLR2, also known as CD282 (cluster of differentiation 282), plays a role in the immune system. It is a membrane protein and a receptor expressed on the surface of certain cells that recognizes foreign substances and transmits appropriate signals to cells of the immune system. The protein encoded by this gene is a member of the Toll-like receptor (TLR) family and plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from fruit flies to humans, sharing structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) expressed on infectious pathogens and mediate the production of cytokines required for effective immune development. Different TLRs exhibit distinct expression patterns. This gene is most abundantly expressed in peripheral blood leukocytes, mediating host responses to Gram-positive bacteria and yeast by stimulating NF-κB. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Saliva, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.3 ★★★★★
Based on 12 reviews
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Product Reviews
★★★★★ 5
Great. Just as good as fancy stuff But dose not pair with peppermint.
Size: 16 Ounce (Pack of 1)
If you're making Macha lattes or mixing the match with anything, this is perfect! No need to buy the expensive ceremonial level stuff unless you're drinking ONLY Macha. This is honestly perfect for everything.
Mix 2 of its provided spoonfuls with 1/2 cup of kettle hot water and stir, maybe even add more matcha until a bit thick, pour over a glass full of ice (should fill half the glass if full of ice) as the last half as milk with a pump of your favorite syrup. Mine is strawberry, my gf's is Vanilla. Peppermint was not good, though fyi.
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Reviewed in the United States on June 1, 2026
★★★★★ 4
Makes It Green; Doesn't Taste as Clean
Size: 16 Ounce (Pack of 1)
It is matcha. It makes things green. For all intents and purposes, this is enough for the typical matcha user. For those with a bit of an upturned nose to anything that's "inferior" to matcha, then this is not for you. By taste, this product is not as good on the tastebuds as, say, Jade Leaf. It doesn't do matcha justice and, in my opinion, does not mix that well when making lattes. In terms of color, this one is also paling in comparison when by itself, but when added to other things as a flavoring agent or colorant, then it does the job as well as it can.
I am not saying this matcha is bad at all. I think if it comes down to drinking, save your money and get some Jade Leaf or equivalent ceremonial grade matcha. You WILL taste the difference. Now if you're going to be making a lot of baked goods or cooked goods? This wouldn't be that bad—you don't want an overbearing matcha taste anyway and the shade of green this produces is not THAT bad.
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Reviewed in the United States on October 19, 2025
★★★★★ 5
Contemporary translation of a set of Christian classics
Format: Hardcover
How did the New Testament come to be formed? What books were left out? What did some of those books say? These are some of the questions on the minds of many curious people today. Too often, local congregations ignore these questions, to the detriment of the church. But these questions about Christian origins are very, very real in the hearts and minds of many people today.
Now, from one of the most significant writers about Christian origins today comes a completely new edition of the Apostolic Fathers -- the letters of Clement, the letters of Ignatius, the letter of Polycarp, the Martyrdom of Polycarp, the Didache, the Epistle of Barnabas, the fragments of Papias and Quadratus, the Epistle to Diognetus, and the Shepherd of Hermas. These were early Christian writings that are generally attributed to church leaders of the late first and early second centuries CE (and, in most cases, are probably authentic). Clement, for example, was an early leader in Rome (circa 96 CE); Ignatius wrote a number of letters to churches in various cities (circa 110 CE), sort of following in the footsteps of Paul.
This two-volume set replaces the classic edition of the Apostolic Fathers in the Loeb Classical Library edited by Kirsopp Lake nearly a century ago. As with all books in the Loeb Classical Library, the original (in this case, Greek) text is presented on the left-hand pages, and an English translation is presented on the right. The text is extremely readable, and the introductions to each of the books are clear, succinct, and to the point.
Some of these books almost made it into the New Testament! The epistles of Clement are found in some early New Testament manuscripts and were widely read in the early churches. The Shepherd of Hermas may have been the most widely read book by early Christians. Several of these books were probably written before some of books in the New Testament. In an age in which people are asking questions about the origins of Christianity and the authority of the scriptures, it is crucial that we have at least a basic understanding of our early history. In my mind, a decent familiarity with the Apostolic Fathers -- as well as the Dead Sea Scrolls and the Nag Hammadi library -- is essential.
This two-volume set is extremely valuable. Ehrman's critical textual work, his contemporary translations, and his up-to-date introductions are a welcome addition to current literature. The trained scholar will delight in having a new critical edition of the Greek text . . . but an ordinary lay reader could also profit tremendously from reading some of these early Christian writings that were not deemed to be sufficiently close to the first generation of apostles to make the cut into the New Testament.
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Reviewed in the United States on August 12, 2006
★★★★★ 5
Great stuff!
Format: Hardcover
Hey evangelicals get off your butts,(or sit down on your butts) and read the fathers of the church you're supposed to be a part of. Great stuff!
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Reviewed in the United States on September 5, 2015
★★★★★ 5
Great book.
Format: Hardcover
Want to know about the Catholic Church read the Fathers, this is only one of the many. Great book.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 22, 2015
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