SKU: 60450668282

Rat TNF-α High Sensitivity ELISA Kit

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Ships within 48 hours · Estimated delivery Aug 26 - Aug 31

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Description

Rat TNF-α High Sensitivity ELISA KitProduct Specification Description Detection Principle: This kit uses double antibody sandwich ELISA technology. Specific anti rat tnf Capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The tnf &alpha in the sample; Binding to solid phase antibodies. After

Product Specification

Description

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti rat tnf-α Capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The tnf-&alpha in the sample; Binding to solid-phase antibodies. After thorough washing to remove free and unbound components, biotinylated detection antibody was added for incubation. After washing again, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color reaction and TNF - &alpha in the sample; There is a positive correlation between the concentration of. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: Pre coated 96 well plates, standards, biotin labeled tnf-α One copy of detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 1.73 pg/ml

Detection Range: 7.81-500 pg/ml

Recovery Range: 92-127%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Tumor necrosis factor alpha; (tnf-α)Also known as cachexia and tnfsf1a, it is an adipokine that participates in systemic inflammation and is also one of the cytokines that stimulate the acute phase response. It is mainly produced by activated macrophages, but also secreted by other types of cells, such as cd4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils and neurons. It has a variety of regulatory functions in the immune response and can also serve as a potential pyrogen. Tnf-α After responding to stimuli (infectious agents or tissue damage), it activates neutrophils in the systemic circulation, changes the characteristics of vascular endothelial cells, regulates the metabolic activity of other tissues, and exhibits tumor killing activity by inducing local coagulation. Tnf-α It plays an important role in the pathogenesis of inflammation in joint tissues and other tissues. Recently, more and more information shows that tnf-α It is also involved in the pathogenesis of AIDS. Tnf-α The assay is also very useful for transplantation research.

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SKU: 60450668282

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William Lovelace
New York, US
★★★★★ 5
Great dog toys
Color: S6-muti-color
These are long lasting, durable dog toys. My dogs love the squeak sound.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 23, 2026
K
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Karen
Battle Creek, US
★★★★★ 5
Durable
Color: S6-muti-color
The balls are great for chewers. The squeaky didn’t last long but they are perfect for the ball launcher.
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Reviewed in the United States on May 18, 2026
C
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Candice K
Louisville, US
★★★★★ 4
The squeeker isn't durable, but the balls themselves are.
Color: S6-muti-color, Color: S6-muti-color
I have two 70+lb, ball obsessed dogs (a field bred Golden Retriever, and an Old Time Scotch Collie), and these balls are living up to the abuse they're receiving. In the fall we always lose a ball or two in the yard due to getting lost in leaves or longer grass, so I always try and buy a multi pack at the start of the season, so went with these this year. I'm glad I did. Great price for a 6 pack, they fit the medium Chuck-It launcher (a little loosely, but they throw just fine), have great bounce, they FLOAT (which means I didn't lose any at the beach), and they're super durable to the dreaded "mouth squish." The squeaker dies pretty quick, like, within a day, but it isn't the kind that can fall out or choke your dog, so no worries there. The boys are happy, I'm happy, my wallet is happy - I've already ordered another pack.
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Reviewed in the United States on November 2, 2024
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S. Berg
San Leandro, US
★★★★★ 5
Good balls
Color: S6-glow
My dog is a chewer, and these have held up for close to a year actually a pretty good product
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Reviewed in the United States on May 27, 2026
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nanny america
Belleville, US
★★★★★ 3
Disappointed
Color: S6-muti-color
These were great for about 20 minutes then they stopped squeaking...we have 2 left that I have saved but the other 4 do not squeak anymore...disappointed as my dog loves to squeak things...would not buy these again...My dog is only an 8lb dog too...but he can still play with the balls without the squeak...
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Reviewed in the United States on April 26, 2026

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